Exploring the host range for genetic transfer of magnetic organelle biosynthesis

Dziuba, Marina V.; Müller, Frank-Dietrich; Pósfai, Mihály; Schüler, Dirk

doi:10.1038/s41565-023-01500-5

Cited by 4 publications

(1 citation statement)

References 56 publications

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“…Although whether MGC-containing bacteria identified here are truly magnetotactic needs further investigations, considering that transcriptionally silent magnetosome genes may have the potential to be activated under certain conditions 69 , the unexpectedly high relative abundance of MGC-containing bacteria in freshwater microbiota and the predominance of Deltaproteobacteria in MGC-containing communities not only improve our understanding of diversity and ecology of putative MTB but also highlight the previous underestimation of their roles in global biogeochemical cycling in oxygen-stratified habitats. Moreover, newly identified MGCs from different taxonomic lineages in this study provide valuable functional gene resources for future production of transgenic biogenic magnetic nanoparticles with various sizes, shapes, and physicochemical properties in foreign hosts [70][71][72] .…”

Section: Discussionmentioning

confidence: 99%

The under-recognized dominance of magnetosome gene cluster-containing bacteria in oxygen-stratified freshwater ecosystems

Ji,

Wan,

Goswami

et al. 2024

Preprint

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Magnetotactic bacteria (MTB) capable of magnetosome organelle biomineralization and magnetotaxis are widespread in chemically stratified aquatic environments. Conventionally, it has long been considered that the overall abundance of MTB in microbiota is not very high and that Magnetococcia is the most frequently identified and predominant MTB members. However, the diversity and distribution of MTB in chemically stratified environments remain elusive due to the lack of large-scale systematic analyses. Here we conduct a comprehensive survey of genomes containing magnetosome gene clusters (MGCs), a group of genes responsible for magnetosome biomineralization and magnetotaxis, in 267 metagenomes from 38 oxygen-stratified freshwater environments. A total of 63 MGC-containing genomes belonging to eight bacterial phyla are reconstructed, including the newly identified Myxococcota. We discover an unexpectedly high relative abundance of putative MTB (up to 15.4% of metagenomic reads) in hypoxic and anoxic water columns, in which Deltaproteobacteria, rather than traditionally considered Magnetococcia, are the most ubiquitous and predominant MGC-containing bacteria. Our analysis reveals a depth-specific taxonomy and function of MGC-containing bacteria in stratified water columns shaped by physicochemical conditions. These findings underscore the unrecognized ecophysiological importance of MTB in freshwater ecosystems.

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Section: Discussionmentioning

confidence: 99%

The under-recognized dominance of magnetosome gene cluster-containing bacteria in oxygen-stratified freshwater ecosystems

Ji,

Wan,

Goswami

et al. 2024

Preprint

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Harnessing and Mimicking Bacterial Features to Combat Cancer: From Living Entities to Artificial Mimicking Systems

Gao,

Duan,

et al. 2024

Advanced Materials

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Bacterial‐derived micro‐/nanomedicine has garnered considerable attention in anticancer therapy, owing to the unique natural features of bacteria, including specific targeting ability, immunogenic benefits, physicochemical modifiability, and biotechnological editability. Besides, bacterial components have also been explored as promising drug delivery vehicles. Harnessing these bacterial features, cutting‐edge physicochemical and biotechnologies have been applied to attenuated tumor‐targeting bacteria with unique properties or functions for potent and effective cancer treatment, including strategies of gene‐editing and genetic circuits. Further, the advent of bacteria‐inspired micro‐/nanorobots and mimicking artificial systems has furnished fresh perspectives for formulating strategies for developing highly efficient drug delivery systems. Focusing on the unique natural features and advantages of bacteria, this review delves into advances in bacteria‐derived drug delivery systems for anticancer treatment in recent years, which has experienced a process from living entities to artificial mimicking systems. Meanwhile, a summary of relative clinical trials is provided and primary challenges impeding their clinical application are discussed. Furthermore, future directions are suggested for bacteria‐derived systems to combat cancer.

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Magnetic Resonance Imaging of Mammalian Cells Individually Expressing Membrane-Associated Magnetosome Proteins I, L, B, and E

Sun,

Varela-Mattatall,

Vivekanantha

et al. 2024

Mol Imaging

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The magnetosome, a membrane-bound iron biomineral formed within magnetotactic bacteria, is a unique model for a magnetic resonance imaging (MRI) reporter gene contrast agent. We are translating this technology to mammalian cells by expressing essential magnetosome genes mamI, mamL, mamB, and mamE into MDA-MB-435 human melanoma cells. Currently, these genes are individually expressed in the cell although the future goal is to express all four genes together. We examined the influence of these genes on cellular MRI relaxation rates by culturing cells in the presence and absence of iron-supplemented medium and scanning them at 3 Tesla using a gelatin phantom. Total cellular iron was measured by inductively-coupled plasma mass spectrometry and correlated with relaxation rates obtained from phantom experiments. Apart from mamE, magnetosome genes that are individually expressed in mammalian cells grown in iron supplement significantly affected cellular transverse relaxation rates compared to cells grown without iron supplement. Interestingly, mamI, mamL, mamB, and mamE (even though the latter had no effect on relaxation rate) significantly affected cellular iron content. This developing gene-based contrast agent will equip MRI with improvement to imaging sensitivity and the technology to track cellular activities long term.

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Exploring the host range for genetic transfer of magnetic organelle biosynthesis

Cited by 4 publications

References 56 publications

The under-recognized dominance of magnetosome gene cluster-containing bacteria in oxygen-stratified freshwater ecosystems

The under-recognized dominance of magnetosome gene cluster-containing bacteria in oxygen-stratified freshwater ecosystems

Harnessing and Mimicking Bacterial Features to Combat Cancer: From Living Entities to Artificial Mimicking Systems

Magnetic Resonance Imaging of Mammalian Cells Individually Expressing Membrane-Associated Magnetosome Proteins I, L, B, and E

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