Exploring the effects of the crosslink density on the physicochemical properties of collagen-based scaffolds

Salvatore, Luca; Calò, Emanuela; Bonfrate, Valentina; Pedone, Deborah; Gallo, Nunzia; Natali, Maria Lucia; Sannino, Alessandro; Madaghiele, Marta

doi:10.1016/j.polymertesting.2020.106966

Cited by 19 publications

(9 citation statements)

References 44 publications

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“…Collagenase degradation was especially sensitive to ρxel, making dehydrothermal CL degradation faster (1 h) than that observed for carbodiimide (4 h), while aldehyde treated samples showed negligible weight loss. A correlation was found between water uptake and ρxel and the same was observed for in vitro half-life of crosslinked collagen scaffolds [86].…”

Section: Collagensupporting

confidence: 76%

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Biomaterials for Soft Tissue Repair and Regeneration: A Focus on Italian Research in the Field

et al. 2021

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Tissue repair and regeneration is an interdisciplinary field focusing on developing bioactive substitutes aimed at restoring pristine functions of damaged, diseased tissues. Biomaterials, intended as those materials compatible with living tissues after in vivo administration, play a pivotal role in this area and they have been successfully studied and developed for several years. Namely, the researches focus on improving bio-inert biomaterials that well integrate in living tissues with no or minimal tissue response, or bioactive materials that influence biological response, stimulating new tissue re-growth. This review aims to gather and introduce, in the context of Italian scientific community, cutting-edge advancements in biomaterial science applied to tissue repair and regeneration. After introducing tissue repair and regeneration, the review focuses on biodegradable and biocompatible biomaterials such as collagen, polysaccharides, silk proteins, polyesters and their derivatives, characterized by the most promising outputs in biomedical science. Attention is pointed out also to those biomaterials exerting peculiar activities, e.g., antibacterial. The regulatory frame applied to pre-clinical and early clinical studies is also outlined by distinguishing between Advanced Therapy Medicinal Products and Medical Devices.

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Section: Collagensupporting

confidence: 76%

“…For example, a well-known question is related to the use of aldehydes. In all cases, the effect of crosslinking on the ability of CL to cell attachment properties must be verified, as the cell-binding domains can be less exposed after the crosslinking step [ 86 ].…”

Section: Collagenmentioning

confidence: 99%

Biomaterials for Soft Tissue Repair and Regeneration: A Focus on Italian Research in the Field

et al. 2021

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“…The aqueous extract was collected as the supernatant after centrifugation at 4000 rpm for 5 min at room temperature. The LYS quantity present in the extract was spectrophotometrically determined by means of a slightly modified TNBS test [ 42 , 43 ]. About 0.2 mL of extract was added to 0.3 mL of 4% ( w / v ) NaHCO 3 solution and 0.5 mL of 0.05% ( w / v ) TNBS.…”

Section: Methodsmentioning

confidence: 99%

Development of L-Lysine-Loaded PLGA Microparticles as a Controlled Release System for Angiogenesis Enhancement

et al. 2023

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Vascularization is a highly conserved and considerably complex and precise process that is finely driven by endogenous regulatory processes at the tissue and systemic levels. However, it can reveal itself to be slow and inadequate for tissue repair and regeneration consequent to severe lesions/damages. Several biomaterial-based strategies were developed to support and enhance vasculogenesis by supplying pro-angiogenic agents. Several approaches were adopted to develop effective drug delivery systems for the controlled release of a huge variety of compounds. In this work, a microparticulate system was chosen to be loaded with the essential amino acid L-lysine, a molecule that has recently gained interest due to its involvement in pro-angiogenic, pro-regenerative, and anti-inflammatory mechanisms. Poly (lactic-co-glycolic acid), the most widely used FDA-approved biodegradable synthetic polymer for the development of drug delivery systems, was chosen due to its versatility and ability to promote neovascularization and wound healing. This study dealt with the development and the effectiveness evaluation of a PLGA-based microparticulate system for the controlled release of L-lysine. Therefore, in order to maximize L-lysine encapsulation efficiency and tune its release kinetics, the microparticle synthesis protocol was optimized by varying some processing parameters. All developed formulations were characterized from a morphological and physicochemical point of view. The optimized formulation was further characterized via the evaluation of its preliminary biological efficacy in vitro. The cellular and molecular studies revealed that the L-lysine-loaded PLGA microparticles were non-toxic, biocompatible, and supported cell proliferation and angiogenesis well by stimulating the expression of pro-angiogenic genes such as metalloproteinase-9, focal adhesion kinases, and different growth factors. Thus, this work showed the potential of delivering L-lysine encapsulated in PLGA microparticles as a cost-effective promoter system for angiogenesis enhancement and rapid healing.

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“…The possibility to define specific scaffolds properties (i.e., by tuning protein concentration, solvent type and concentration, protein molecular weight, superficial morphology, 3D organization, and by pre- and post-production processing) offers a great opportunity to modulate the structure-related biological activity of the scaffolds in order to optimize their capability to induce and sustain tissue regeneration [ 20 , 35 , 43 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ]. Moreover, the use of collagen is advantageous for regenerative medicine and tissue engineering applications because, being recognized as a self-molecule, it is metabolized by the natural body enzymatic apparatus that gradually breaks down collagen molecules and substitutes it with newly synthetized one.…”

Section: Collagen As Biomaterialsmentioning

confidence: 99%

An Update on the Clinical Efficacy and Safety of Collagen Injectables for Aesthetic and Regenerative Medicine Applications

Salvatore¹,

Natali

Brunetti³

et al. 2023

Polymers

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Soft tissues diseases significantly affect patients quality of life and usually require targeted, costly and sometimes constant interventions. With the average lifetime increase, a proportional increase of age-related soft tissues diseases has been witnessed. Due to this, the last two decades have seen a tremendous demand for minimally invasive one-step resolutive procedures. Intensive scientific and industrial research has led to the recognition of injectable formulations as a new advantageous approach in the management of complex diseases that are challenging to treat with conventional strategies. Among them, collagen-based products are revealed to be one of the most promising among bioactive biomaterials-based formulations. Collagen is the most abundant structural protein of vertebrate connective tissues and, because of its structural and non-structural role, is one of the most widely used multifunctional biomaterials in the health-related sectors, including medical care and cosmetics. Indeed, collagen-based formulations are historically considered as the “gold standard” and from 1981 have been paving the way for the development of a new generation of fillers. A huge number of collagen-based injectable products have been approved worldwide for clinical use and have routinely been introduced in many clinical settings for both aesthetic and regenerative surgery. In this context, this review article aims to be an update on the clinical outcomes of approved collagen-based injectables for both aesthetic and regenerative medicine of the last 20 years with an in-depth focus on their safety and effectiveness for the treatment of diseases of the integumental, gastrointestinal, musculoskeletal, and urogenital apparatus.

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Exploring the effects of the crosslink density on the physicochemical properties of collagen-based scaffolds

Cited by 19 publications

References 44 publications

Biomaterials for Soft Tissue Repair and Regeneration: A Focus on Italian Research in the Field

Biomaterials for Soft Tissue Repair and Regeneration: A Focus on Italian Research in the Field

Development of L-Lysine-Loaded PLGA Microparticles as a Controlled Release System for Angiogenesis Enhancement

An Update on the Clinical Efficacy and Safety of Collagen Injectables for Aesthetic and Regenerative Medicine Applications

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