Exploring the effects of methylene blue on amyloid fibrillogenesis of lysozyme

How, Su Chun; Cheng, Yu Hong; Lo, Chun Hsien; Lai, Jinn Tsyy; Lin, Ta Hsien; Bednarikova, Zuzana; Antošová, Andrea; Gažová, Zuzana; Wu, Josephine W.; Wang, Steven S.‐S.

doi:10.1016/j.ijbiomac.2018.08.038

Cited by 22 publications

(15 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this work we examine five amyloidophilic dye molecules possessing very characteristic spectral properties – thioflavin-T, Congo red, 8-anilinonaphthalene-1-sulfonic acid, dapoxyl (Dap) 33 and methylene blue (MB) 34,35 in order to determine their cross-interactions when binding to insulin amyloid fibrils. We show the changes of absorbance spectra when these molecules interact with one another in solution and on fibrils, as well as how they influence each other’s fibril binding affinity and the fluorescence intensity of ThT, ANS and Dap.…”

Section: Introductionmentioning

confidence: 99%

Amyloidophilic Molecule Interactions on the Surface of Insulin Fibrils: Cooperative Binding and Fluorescence Quenching

Žiaunys

Mikalauskaite

Smirnovas

2019

Sci Rep

View full text Add to dashboard Cite

Protein aggregation into insoluble fibrillar aggregates is linked to several neurodegenerative disorders, such as Alzheimer’s or Parkinson’s disease. Commonly used methods to study aggregation inhibition or fibril destabilization by potential drugs include spectroscopic measurements of amyloidophilic dye molecule fluorescence or absorbance changes. In this work we show the cross-interactions of five different dye molecules on the surface of insulin amyloid fibrils, resulting in cooperative binding and fluorescence quenching.

show abstract

Section: Introductionmentioning

confidence: 99%

Amyloidophilic Molecule Interactions on the Surface of Insulin Fibrils: Cooperative Binding and Fluorescence Quenching

Žiaunys

Mikalauskaite

Smirnovas

2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…This effect has been attributed to the special anti-inflammatory property of MB [11]. Some particular studies suggested that doses of 60 mg of MB administered three times per day seemed to be efficient for this therapeutic purpose; yet, additional data obtained from clinical trials are needed to allow the use of this dye in therapy schemes for neurological disorders [18,48]. MB is used also for prevention of urinary tract infection in elderly patients, with a dosage of 3 × 65 mg per day applied in this case [19].…”

Section: Preoperative and Intraoperative Use In Cardiac Surgerymentioning

confidence: 99%

“…There are some clinical applications approved by the Food and Drug Administration (FDA) for MB among which are the treatments of methemoglobinemia, where in inhered methemoglobinemia daily doses of 50-250 mg are used for a lifetime; in acute methemoglobinemia dosages of 1.3 mg/kg are used once or twice per day with intravenous (i.v.) administration over 20 min [18]; in septic shock, 200 mg are i.v. administrated over 1 h, followed by infusion of 0.25-2 mg/kg/h; and for ifosfamide-induced encephalopathy, doses of 4 × 50 mg/day per os (p.o.)…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications

et al. 2020

View full text Add to dashboard Cite

Methylene blue and proflavine are fluorescent dyes used to stain nucleic acid from the molecular level to the tissue level. Already clinically used for sentinel node mapping, detection of neuroendocrine tumors, methemoglobinemia, septic shock, ifosfamide-induced encephalopathy, and photodynamic inactivation of RNA viruses, the antimicrobial, anti-inflammatory, and antioxidant effect of methylene blue has been demonstrated in different in vitro and in vivo studies. Proflavine was used as a disinfectant and bacteriostatic agent against many gram-positive bacteria, as well as a urinary antiseptic involved in highlighting cell nuclei. At the tissue level, the anti-inflammatory effects of methylene blue protect against pulmonary, renal, cardiac, pancreatic, ischemic-reperfusion lesions, and fevers. First used for their antiseptic and antiviral activity, respectively, methylene blue and proflavine turned out to be excellent dyes for diagnostic and treatment purposes. In vitro and in vivo studies demonstrated that both dyes are efficient as perfusion and tissue tracers and permitted to evaluate the minimal efficient concentration in different species, as well as their pharmacokinetics and toxicity. This review aims to identify the optimal concentrations of methylene blue and proflavine that can be used for in vivo experiments to highlight the vascularization of the skin in the case of a perforasome (both as a tissue tracer and in vascular mapping), as well as their effects on tissues. This review is intended to be a comparative and critical presentation of the possible applications of methylene blue (MB) and proflavine (PRO) in the surgical field, and the relevant biomedical findings from specialized literature to date are discussed as well.

show abstract

“…In this work, we aim to investigate the modulation effect of tannic acid on amyloid fibrillation using hen egg white lysozyme as a model amyloid system. Hen egg white lysozyme is a widely used model system in amyloid research [18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34]. Tannic acid is a type of polyphenol, as shown in Figure 1, and many polyphenol compounds including tannic acid have been found to possess amyloid-inhibition capabilities [35].…”

Section: Introductionmentioning

confidence: 99%

Tannic Acid-Induced Surface-Catalyzed Secondary Nucleation during the Amyloid Fibrillation of Hen Egg-White Lysozyme

Tian

Yang

Wang

et al. 2018

IJMS

View full text Add to dashboard Cite

Amyloid fibrillation by hen egg white lysozyme under the influence of tannic acid was investigated by atomic force microscopy and fluorescence spectroscopy. Tannic acid was found to be able to induce the formation of amyloid fibrils with an interesting mixed morphology. Such morphology features with the existence of areas of thickening alternating with areas of normal height. This novel modulation effect of tannic acid on amyloid fibrillation was interpreted by the established surface-catalyzed secondary nucleation theory. We further performed a fluorescence quenching study to investigate the intermolecular interaction between tannic acid and lysozyme. The results support that lysozyme and tannic acid interact with each other mainly through hydrophobic interactions. We also discussed why hydrogen-bonding interaction is not a dominant factor in the interaction between tannic acid and lysozyme though tannic acid contains a significant amount of hydroxyl groups. Our work provides new insight into the effect of tannic acid, a well-known amyloid inhibitor, on amyloid fibrillation.

show abstract

Exploring the effects of methylene blue on amyloid fibrillogenesis of lysozyme

Cited by 22 publications

References 57 publications

Amyloidophilic Molecule Interactions on the Surface of Insulin Fibrils: Cooperative Binding and Fluorescence Quenching

Amyloidophilic Molecule Interactions on the Surface of Insulin Fibrils: Cooperative Binding and Fluorescence Quenching

Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications

Tannic Acid-Induced Surface-Catalyzed Secondary Nucleation during the Amyloid Fibrillation of Hen Egg-White Lysozyme

Contact Info

Product

Resources

About