Exploring the Effect of High-Energy Heavy Ion Beam on Rice Genome: Transposon Activation

Wen, Xiaoting; Li, Jingpeng; Yang, Fu; Zhang, Xin; Li, Yiwei

doi:10.3390/genes14122178

Cited by 1 publication

(1 citation statement)

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“…This could be a beneficial view in inducing deletions in the plant genome. Recently, mutation analysis has been effectively conducted by using whole genome sequencing and exome sequencing in several plant species ( Li et al., 2017 ; Ichida et al., 2019 ; Li et al., 2019 ; Hase et al., 2020 ; Liu et al., 2023 ; Yang et al., 2019 ; Oono et al., 2020 ; Zheng et al., 2020 , 2021 ; Sun et al., 2022 ; Ren et al., 2023 ; Wen et al., 2023 ; Xiong et al., 2023 ). By using whole-genome mutation analysis, we previously found a significant difference in mutation spectrum between 30 keV μm -1 and 290 keV μm -1 heavy-ion-beam irradiations ( Hirano et al., 2015 ; Kazama et al., 2017 ; Morita et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic view of heavy-ion-induced deletions associated with distribution of essential genes in Arabidopsis thaliana

Ishii,

Kazama,

Hirano

et al. 2024

Front. Plant Sci.

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Heavy-ion beam, a type of ionizing radiation, has been applied to plant breeding as a powerful mutagen and is a promising tool to induce large deletions and chromosomal rearrangements. The effectiveness of heavy-ion irradiation can be explained by linear energy transfer (LET; keV µm-1). Heavy-ion beams with different LET values induce different types and sizes of mutations. It has been suggested that deletion size increases with increasing LET value, and complex chromosomal rearrangements are induced in higher LET radiations. In this study, we mapped heavy-ion beam-induced deletions detected in Arabidopsis mutants to its genome. We revealed that deletion sizes were similar between different LETs (100 to 290 keV μm-1), that their upper limit was affected by the distribution of essential genes, and that the detected chromosomal rearrangements avoid disrupting the essential genes. We also focused on tandemly arrayed genes (TAGs), where two or more homologous genes are adjacent to one another in the genome. Our results suggested that 100 keV µm-1 of LET is enough to disrupt TAGs and that the distribution of essential genes strongly affects the heritability of mutations overlapping them. Our results provide a genomic view of large deletion inductions in the Arabidopsis genome.

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