Exploring the correlation between deltamethrin stress and Keap1-Nrf2-ARE pathway from Drosophila melanogaster RNASeq data

Liu, Yahui; Ge, Mengying; Zhang, Tingting; Chen, Lu; Xing, Yu-Ping; Liu, Liu; Li, Fengliang; Cheng, Lijun

doi:10.1016/j.ygeno.2019.07.019

Cited by 13 publications

(6 citation statements)

References 45 publications

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“…2001); the loss of Ugt302e1 , whose function is currently unknown, and three cytochrome P450s ( Cyp4ad1, Cyp4d1, and Cyp12g1 ). While there is no ortholog of Cyp12g1 in D. melanogaster , both Cyp4ad1 and Cyp4d1 are upregulated in response to the pyrethroid deltamethrin (Liu et al . 2020).…”

Section: Resultsmentioning

confidence: 99%

“…Only five herbivore-specific changes in copy number were found among the detoxification gene families: the duplication of GstS1, which has a role in oxidative stress responses and in flight muscle structure (Singh et al 2001); the loss of Ugt302e1, whose function is currently unknown, and three cytochrome P450s (Cyp4ad1, Cyp4d1, and Cyp12g1). While there is no ortholog of Cyp12g1 in D. melanogaster, both Cyp4ad1 and Cyp4d1 are upregulated in response to the pyrethroid deltamethrin (Liu et al 2020).…”

Section: Contractions and Expansions Of Chemosensory And Detoxificati...mentioning

confidence: 99%

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Evolution of chemosensory and detoxification gene families across herbivorous Drosophilidae

Peláez

Gloss

Goldman-Huertas

et al. 2023

Preprint

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Herbivorous insects are exceptionally diverse, accounting for a quarter of all known eukaryotic species, but the genetic basis of adaptations that enabled this dietary transition remains poorly understood. Many studies have suggested that expansions and contractions of chemosensory and detoxification gene families - genes directly mediating interactions with plant chemical defenses - underlie successful plant colonization. However, this hypothesis has been challenging to test because the origins of herbivory in many lineages are ancient (>150 million years ago [mya]), obscuring genomic evolutionary patterns. Here, we characterized chemosensory and detoxification gene family evolution across Scaptomyza, a genus nested within Drosophila that includes a recently derived (<15 mya) herbivore lineage of mustard (Brassicales) specialists and carnation (Caryophyllaceae) specialists, and several non-herbivorous species. Comparative genomic analyses revealed that herbivorous Scaptomyza have among the smallest chemosensory and detoxification gene repertoires across 12 drosophilid species surveyed. Rates of gene turnover averaged across the herbivore clade were significantly higher than background rates in over half of the surveyed gene families. However, gene turnover was more limited along the ancestral herbivore branch, with only gustatory receptors and odorant binding proteins experiencing strong losses. The genes most significantly impacted by gene loss, duplication, or changes in selective constraint were those involved in detecting compounds associated with feeding on plants (bitter or electrophilic phytotoxins) or their ancestral diet (yeast and fruit volatiles). These results provide insight into the molecular and evolutionary mechanisms of plant-feeding adaptations and highlight strong gene candidates that have also been linked to other dietary transitions in Drosophila.

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Section: Resultsmentioning

confidence: 99%

Section: Contractions and Expansions Of Chemosensory And Detoxificati...mentioning

confidence: 99%

Evolution of chemosensory and detoxification gene families across herbivorous Drosophilidae

Peláez

Gloss

Goldman-Huertas

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Mod(mdg4) variants bind a variety of insulators (DNA domains involved in nuclear organization and higher order chromatin structures) ( Ghosh et al 2001 , Melnikova et al 2004 , Golovnin et al 2007 ) and have been involved in regulating numerous traits of the insect embryonic progression such as synapsis structure ( Gorczyca et al 1999 ), chromosome Y-linked testis development ( Branco et al 2013 ), and midgut maturation ( Cai et al 2012 ). Changes in expression of Mod(mdg4) have been reported in Drosophila Kc cells treated with deltamethrin ( Liu et al 2020 ). The downstream targets of mod (mdg4) have also been shown to affect the down regulation of proteins associated with protection from various stress conditions, therefore acting as modulators of cytotoxic damage in Drosophila ( Lin et al 2021 ).…”

Section: Discussionmentioning

confidence: 97%

Expansive and Diverse Phenotypic Landscape of Field Aedes aegypti (Diptera: Culicidae) Larvae with Differential Susceptibility to Temephos: Beyond Metabolic Detoxification

Morgan

Salcedo-Sora

Triana-Chávez

et al. 2021

Journal of Medical Entomology

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Arboviruses including dengue, Zika, and chikungunya are amongst the most significant public health concerns worldwide. Arbovirus control relies on the use of insecticides to control the vector mosquito Aedes aegypti (Linnaeus), the success of which is threatened by widespread insecticide resistance. The work presented here profiled the gene expression of Ae. aegypti larvae from field populations of Ae. aegypti with differential susceptibility to temephos originating from two Colombian urban locations, Bello and Cúcuta, previously reported to have distinctive disease incidence, socioeconomics, and climate. We demonstrated that an exclusive field-to-lab (Ae. aegypti strain New Orleans) comparison generates an over estimation of differential gene expression (DGE) and that the inclusion of a geographically relevant field control yields a more discrete, and likely, more specific set of genes. The composition of the obtained DGE profiles is varied, with commonly reported resistance associated genes including detoxifying enzymes having only a small representation. We identify cuticle biosynthesis, ion exchange homeostasis, an extensive number of long noncoding RNAs, and chromatin modelling among the differentially expressed genes in field resistant Ae. aegypti larvae. It was also shown that temephos resistant larvae undertake further gene expression responses when temporarily exposed to temephos. The results from the sampling triangulation approach here contribute a discrete DGE profiling with reduced noise that permitted the observation of a greater gene diversity, increasing the number of potential targets for the control of insecticide resistant mosquitoes and widening our knowledge base on the complex phenotypic network of the Ae. aegypti response to insecticides.

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“…Mod(mdg4) variants bind a variety of insulators (DNA domains involved in nuclear organization and higher order chromatin structures) [116–118] and have been involved in regulating numerous traits of the insect embryonic progression such as synapsis structure [119], chromosome Y-linked testis development [120], and mid-gut maturation [121]. Changes in expression of Mod(mdg4) have been reported in Drosophila Kc cells treated with deltamethrin [122]. The identification of under expression of the mdg4-binding protein in temephos resistant larvae suggests a further role for this protein in mediating insecticide resistance.…”

Section: Discussionmentioning

confidence: 99%

Expansive and diverse phenotypic landscape of field Aedes aegypti larvae with differential susceptibility to temephos: beyond metabolic detoxification

Morgan

Salcedo-Sora

Triana-Chávez

et al. 2021

Preprint

View full text Add to dashboard Cite

Arboviruses including dengue, Zika and chikungunya are amongst the most significant public health concerns worldwide and their control relies heavily on the use of insecticides to control the vector mosquito Aedes aegypti. The success of controlling these vector-pathogen systems is threatened by widespread insecticide resistance. The work presented here profiled the gene expression of the larvae from two field populations of Ae. aegypti with differential susceptibility to temephos. The contrasting phenotypes originated from two Colombian urban locations, Bello and Cucuta, that we have previously reported to have distinctive disease incidence, socioeconomics, and climate. The closeness of the geographical origin of the study populations was suspected to be highly influential in the profiling of the gene expression of resistance since the mosquito resistance levels themselves are highly dependent upon environmental variables. We demonstrated that an exclusive field-to-lab (Ae. aegypti reference strain New Orleans) comparison generates an over estimation of differential gene expression (DGE) and that the inclusion of a geographically relevant field control, as used here, yields a more discrete, and likely, more specific set of genes. The composition of the obtained DGE profiles is varied, with commonly reported resistance associated genes such as detoxifying enzymes having only a small representation. We identify cuticle biosynthesis, ion exchange homeostasis, an extensive number of long non-coding RNAs, and chromatin modelling among the specifically and differentially expressed genes in field resistant Ae. aegypti larvae. It was also shown that temephos resistant larvae undertake further gene expression responses when temporarily exposed to this insecticide. The results from the sampling triangulation approach undertaken here contributes a discrete DGE profiling with reduced noise that permitted the observation of a greater gene diversity. This deeper gene granularity significantly increases the number of potential targets for the control of insecticide resistant mosquitoes and widens our knowledge base on the complex phenotypic network of the Ae. aegypti mosquito responses to insecticides.

show abstract

Exploring the correlation between deltamethrin stress and Keap1-Nrf2-ARE pathway from Drosophila melanogaster RNASeq data

Cited by 13 publications

References 45 publications

Evolution of chemosensory and detoxification gene families across herbivorous Drosophilidae

Evolution of chemosensory and detoxification gene families across herbivorous Drosophilidae

Expansive and Diverse Phenotypic Landscape of Field Aedes aegypti (Diptera: Culicidae) Larvae with Differential Susceptibility to Temephos: Beyond Metabolic Detoxification

Expansive and diverse phenotypic landscape of field Aedes aegypti larvae with differential susceptibility to temephos: beyond metabolic detoxification

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