Exploring the chondroprotective effect of Chaenomeles speciosa on Glucose-6-Phosphate Isomerase model mice using an integrated approach of network pharmacology and experimental validation

Exploring the potential molecular mechanism of Gualou Guizhi decoction in the treatment of rheumatoid arthritis based on network pharmacology and molecular docking

“…After molecular docking by Autodock vina 1.1.2 software, the binding energy between each active ingredient and the receptor protein was obtained, the lower the binding energy of the ligand and receptor docking indicated a more solid molecular binding and a higher possibility of interaction, the binding was better at binding energy ≤ −5.0 kcal-mol −1 and very good at binding energy ≤ −7.5 kcal-mol −1 , [ 11 , 12 ] the docking results were visualized and processed with Strawberry Perl, PyMOL software, and the results are tabulated and summarized in Figures 10 and 11 (As can be seen from Supplementary material 5, http://links.lww.com/MD/L274 , ingredient A is contained in 4 kinds of Traditional Chinese Medicine, so ingredient A is more important. As can be seen from Figure 3 , component D has the most connections with core genes, so component D is relatively important.…”

“…RA belongs to the category of "paralysis," which is often caused by wind, cold, and dampness, resulting in blockage of the meridians, stagnation of the yin and yang, and blockage of the qi and blood, resulting in joint pain, while GGD can establish a method to benefit the yin and regulate the yin, and penetrate the evil to pass the paralysis, and its potential mechanism to intervene in RA needs to be explored. [7,12,15] In this study, 49 targets were screened for the intersection of GGD components and RA, and 30 core targets were identified, including VEGFA, IL1B, IL6, CXCL8, CCL2, JUN, etc. IL-1B promotes the expression of acid-sensing ion channel 1a (ASIC1a) through the activation of mitogen-activated protein kinase (MAPK) and NF-κB signaling, resulting in bone and chondrocyte damage in RA.…”

“…RA belongs to the category of “paralysis,” which is often caused by wind, cold, and dampness, resulting in blockage of the meridians, stagnation of the yin and yang, and blockage of the qi and blood, resulting in joint pain, while GGD can establish a method to benefit the yin and regulate the yin, and penetrate the evil to pass the paralysis, and its potential mechanism to intervene in RA needs to be explored. [ 7 , 12 , 15 ]…”

Recent Advances in Polysaccharides from Chaenomeles speciosa (Sweet) Nakai.: Extraction, Purification, Structural Characteristics, Health Benefits, and Applications

“…Its fruit is a valuable source of healthy food due to its unique taste and rich nutrients such as sugars, proteins, fats, minerals, vitamins A and C, pectin, and malic acid [5]. Furthermore, its fruit contains a variety of natural active components and exhibits a wide range of health benefits, which are clinically used for the treatment of colds, asthma, hepatitis, and rheumatoid arthritis [6,7]. In addition, applying the sap of C. speciosa to ulcerated skin can alleviate ulcers and accelerate wound healing.…”