Exploring the capacity for anaerobic biodegradation of polycyclic aromatic hydrocarbons and naphthenic acids by microbes from oil-sands-process-affected waters

Folwell, Benjamin D.; McGenity, Terry J.; Price, Andrew; Johnson, R. Jeremy; Whitby, Corinne

doi:10.1016/j.ibiod.2014.12.016

Cited by 53 publications

(14 citation statements)

References 48 publications

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“…In contrast, other studies investigating NA biodegradation by biofilms found no evidence of A1CA biodegradation (Demeter et al , ). Furthermore, under low oxygen (Ahad et al , ) and anaerobic (Folwell et al , ) conditions, A1CA was found to persist despite the incubation conditions being amenable for the biodegradation of other compounds such as cyclohexanecarboxylic acid and 1,2‐cyclohexanedicarboxylic acid (Ahad et al , ) or 2‐methylnaphthalene (Folwell et al , ). In addition, the biotransformation of A1CA in our study was faster (although non‐significantly so) than the more‐branched 3EA.…”

Section: Resultsmentioning

confidence: 99%

Diamondoids are not forever: microbial biotransformation of diamondoid carboxylic acids

Folwell

McGenity

Whitby

2019

Microbial Biotechnology

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Summary Oil sands process‐affected waters (OSPW) contain persistent, toxic naphthenic acids (NAs), including the abundant yet little‐studied diamondoid carboxylic acids. Therefore, we investigated the aerobic microbial biotransformation of two of the most abundant, chronically toxic and environmentally relevant diamondoid carboxylic acids: adamantane‐1‐carboxylic acid (A1CA) and 3‐ethyl adamantane carboxylic acid (3EA). We inoculated into minimal salts media with diamondoid carboxylic acids as sole carbon and energy source two samples: (i) a surface water sample (designated TPW) collected from a test pit from the Mildred Lake Settling Basin and (ii) a water sample (designated 2 m) collected at a water depth of 2 m from a tailings pond. By day 33, in TPW enrichments, 71% of A1CA and 50% of 3EA was transformed, with 50% reduction in EC20 toxicity. Similar results were found for 2 m enrichments. Biotransformation of A1CA and 3EA resulted in the production of two metabolites, tentatively identified as 2‐hydroxyadamantane‐1‐carboxylic acid and 3‐ethyladamantane‐2‐ol respectively. Accumulation of both metabolites was less than the loss of the parent compound, indicating that they would have continued to be transformed beyond 33 days and not accumulate as dead‐end metabolites. There were shifts in bacterial community composition during biotransformation, with Pseudomonas species, especially P. stutzeri, dominating enrichments irrespective of the diamondoid carboxylic acid. In conclusion, we demonstrated the microbial biotransformation of two diamondoid carboxylic acids, which has potential application for their removal and detoxification from vast OSPW that are a major environmental threat.

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Section: Resultsmentioning

confidence: 99%

Diamondoids are not forever: microbial biotransformation of diamondoid carboxylic acids

Folwell

McGenity

Whitby

2019

Microbial Biotechnology

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“…In the current study, we observed large differences in the taxonomic distribution between the rare and abundant bacterial subcommunities (Figure ; Table ). For example, abundant taxa, representing 76 genera in total, primarily belonged to Thiobacillus , Thauera , Hydrogenophaga and Arenimonas and were associated with sulphur metabolism or the degradation of organic pollutants (Folwell, McGenity, Price, Johnson, & Whitby, ; Waksman & Joffe, ; Yang, Huang, Chao, & Chang, ). Rare taxa belonged to 394 genera, including Clostridium , Anaerolinea , Planctomyces and Desulfovibrio .…”

Section: Discussionmentioning

confidence: 99%

“…Abundant taxa were ubiquitous across contaminated sites, regardless of local environmental variations, suggesting oil contamination strongly selects for particular taxa. For instance, Hydrogenophaga and Aeromonas are reportedly more abundant in the presence of high concentrations of organic pollutants and may be involved in their degradation (Folwell et al., ; Yang et al., ).…”

Section: Discussionmentioning

confidence: 99%

Biogeography and ecological diversity patterns of rare and abundant bacteria in oil‐contaminated soils

2017

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Revealing the biogeographies and ecologies of rare and abundant microorganisms is crucial to understand ecosystem diversity and function. In this study, we investigated the biogeographic assemblies and ecological diversity patterns of rare and abundant bacteria in long-term oil-contaminated soils at intervals of 46-360 km by performing high-throughput sequencing of 16S rRNA genes. The results clearly revealed distinct distribution patterns for rare and abundant bacteria in soil samples. Rare taxa were unevenly distributed; however, abundant taxa were ubiquitous across all samples. Both rare and abundant subcommunities showed significant distance-decay relationships, and their assemblies were driven by different factors. The rare subcommunity primarily exhibited a spatially structured distribution (i.e., stochastic processes), while edaphic factors (i.e., deterministic processes) largely contributed to the structure of the abundant subcommunity. A network analysis revealed closer relationships between abundant bacteria and their heightened influence on other co-occurrences in the community compared with rare species. In conclusion, rare microbial taxa may play potential roles in maintaining ecosystem diversity, although they do not appear to be central to microbial networks. Abundant microbes are vital for microbial co-occurrences in oil-contaminated soils, and high relative abundance and ubiquitous distribution suggest potential roles in the degradation of organic pollutants.

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“…Many autochthonous microorganisms in polluted soil are competent of degrading hydrocarbon compounds. Biodegradation of hydrocarbons in contaminated soils, which relies on the ability of bacteria, fungi and algae to degrade hydrocarbons components has been established as sustainable and efficient [5]. The ability of microorganisms to degrade hydrocarbons might not be unconnected with the fact that hydrocarbons are natural and neutral compounds [6].…”

Section: Introductionmentioning

confidence: 99%

Degradation Kinetics of Microbial Consortium Isolated from Diesel Oil Impacted Soil in Delta Park, University of Port Harcourt, Nigeria

Udume

Effiong

Abu

2019

MRJI

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Heavy-duty generators constitute a form of hydrocarbon pollution but enrich microorganisms to having degradative ability of hydrocarbons hence can be used for remediation. In this study a laboratory treatability (biodegradation screening) study was employed to investigate the hydrocarbon degradation competence of bacteria and fungi. Culture-dependent microbiological and physicochemical analyses was conducted on the soil samples obtained from the polluted site. The total aerobic heterotrophic bacterial and fungi counts increased from 5.0-7.5 (Log10 cfu/mL) between day 0-14 then reduced to 6.5 Log10 cfu/mL. The hydrocarbon-utilizing bacteria increased from 4.5-5.2 (Log10 cfu/mL). Percentage degradation hydrocarbons, attributed to fungi bacteria and bacteria/fungi consortia, were 42.3%, 54% and 70% respectively while the control had 6.0%. The total petroleum hydrocarbon (TPH) removal rate (K) was modelled using the first order kinetics: y=-0.0398x+9.79; K=0.0398d-1. These results, correspond to a degradation efficiency of 70% and t1/2 of 17 days for the bacterial/fungal consortium. The K values for the other setups were 0.019 d-1, 0.0261 d-1 and 0.0022 d-1 with the corresponding degradation efficiencies of 42%, 54% and 6.01% and half-life of 37 days, 27 days and 315 days for fungi, bacteria and control respectively. This result indicates that the use of microbial consortia has high potentials in remediation of hydrocarbons and other pollutants of concern.

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Exploring the capacity for anaerobic biodegradation of polycyclic aromatic hydrocarbons and naphthenic acids by microbes from oil-sands-process-affected waters

Abstract: 26Both polycyclic aromatic hydrocarbons (PAHs) and naphthenic acids (NAs) 27 are natural components of fossil fuels but are also widespread, toxic and 28 environmentally persistent pollutants.

Cited by 53 publications

References 48 publications

Diamondoids are not forever: microbial biotransformation of diamondoid carboxylic acids

Diamondoids are not forever: microbial biotransformation of diamondoid carboxylic acids

Biogeography and ecological diversity patterns of rare and abundant bacteria in oil‐contaminated soils

Degradation Kinetics of Microbial Consortium Isolated from Diesel Oil Impacted Soil in Delta Park, University of Port Harcourt, Nigeria

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