Exploring the basis of the (uncommon) direct hemolysis caused by phopholipase A2 of Micrurus fulvius venom

Fernández, María Laura; Arce-Bejarano, Ruth; Fernández, Julio M.; Camacho, Luis F.; Gutiérrez, José M.; Kuemmel, Daniel; Fidelio, Gerardo D.; Lomonte, Bruno

doi:10.1016/j.toxicon.2018.06.014

Toxicon

2018

DOI: 10.1016/j.toxicon.2018.06.014

|View full text |Cite

Exploring the basis of the (uncommon) direct hemolysis caused by phopholipase A2 of Micrurus fulvius venom

María Laura Fernández

Ruth Arce-Bejarano

Julio M. Fernández

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2022

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, the neurotoxic polypeptide α-bungarotoxin found in the venom of Bungarus multicintictus acts by blocking the nicotinic acetylcholine receptors, resulting in paralysis. 11 Phospholipase A2 (PLA 2 ), found in the venom of several snake species, exerts myotoxic, 12 neurotoxic, 13 hemolytic, 14 and pro-inflammatory 15 animal poisons and venoms, are a viable source of pharmacological agents and lead to the design of novel therapeutic compounds. 16 Humans and other aerobic organisms produce reactive species (RS) as products of their oxygen-dependent mode of life.…”

mentioning

confidence: 99%

Antioxidant and Neuroprotective Effects of the First Tryptophyllin Found in Snake Venom (Bothrops moojeni)

et al. 2022

View full text Add to dashboard Cite

In this study, we report the isolation, characterization, and synthesis of the peptide BmT-2 belonging to the tryptophyllins family, isolated from the venom of the snake Bothrops moojeni. This is the first time a tryptophyllin is identified in snake venom. We tested whether BmT-2 had cytotoxic effects and antioxidant activity in a set of experiments that included both in vitro and cell-based assays. BmT-2 presented a radical scavenging activity toward ABTS• and AAPH-derived radicals. BmT-2 protected fluorescein, DNA molecules, and human red blood cells (RBCs) from free radicals generated by the thermal decomposition of AAPH. The novel tryptophyllin was not toxic in cell viability tests, where it (up to 0.4 mg/mL) did not cause hemolysis of human RBCs and did not cause significant loss of cell viability, showing a CC50 > 1.5 mM for cytotoxic effects against SK-N-BE(2) neuroblastoma cells. BmT-2 prevented the arsenite-induced upregulation of Nrf2 in Neuro-2a neuroblasts and the phorbol myristate acetate-induced overgeneration of reactive oxygen species and reactive nitrogen species in SK-N-BE(2) neuroblastoma cells. Electronic structure calculations and full atomistic reactive molecular dynamics simulations revealed the relevant contribution of aromatic residues in BmT-2 to its antioxidant properties. Our study presents a novel peptide classified into the family of the tryptophyllins, which has been reported exclusively in amphibians. Despite the promising results on its antioxidant activity and low cytotoxicity, the mechanisms of action of BmT-2 still need to be further elucidated.

show abstract

mentioning

confidence: 99%

Antioxidant and Neuroprotective Effects of the First Tryptophyllin Found in Snake Venom (Bothrops moojeni)

et al. 2022

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Exploring the basis of the (uncommon) direct hemolysis caused by phopholipase A2 of Micrurus fulvius venom

Cited by 1 publication

References 0 publications

Antioxidant and Neuroprotective Effects of the First Tryptophyllin Found in Snake Venom (Bothrops moojeni)

Antioxidant and Neuroprotective Effects of the First Tryptophyllin Found in Snake Venom (Bothrops moojeni)

Contact Info

Product

Resources

About