Exploring the Artemisia Genus: An Insight into the Phytochemical and Multi-Biological Potential of A. campestris subsp. lednicensis (Spreng.) Greuter & Raab-Straube

Abstract

The Artemisia L. genus includes over five hundred species with great economic and medicinal properties. Our study aimed to provide a comprehensive metabolite and bioactivity profile of Artemisia campestris subsp. lednicensis (Spreng.) Greuter & Raab-Straube collected from north-eastern Romania. Liquid chromatography with tandem high-resolution mass spectrometry (LC-HRMS/MS) analysis of different polarity extracts obtained from the aerial parts led to the identification of twelve flavonoids, three phenolic acids, two sesquiterpene lactones, two fatty acids, one coumarin, and one lignan. The antioxidant and enzyme inhibitory properties were shown in the DPPH (0.71–213.68 mg TE/g) and ABTS (20.57–356.35 mg TE/g) radical scavenging, CUPRAC (38.56–311.21 mg TE/g), FRAP (121.68–202.34 mg TE/g), chelating (12.88–22.25 mg EDTAE/g), phosphomolybdenum (0.92–2.11 mmol TE/g), anti-acetylcholinesterase (0.15–3.64 mg GALAE/g), anti-butyrylcholinesterase (0–3.18 mg GALAE/g), anti-amylase (0.05–0.38 mmol ACAE/g), anti-glucosidase (0.43–2.21 mmol ACAE/g), and anti-tyrosinase (18.62–48.60 mg KAE/g) assays. At 100 μg/mL, Artemisia extracts downregulated the secretion of tumor necrosis factor (TNF)-α in a lipopolysaccharide (LPS)-stimulated human neutrophil model (29.05–53.08% of LPS+ control). Finally, the Artemisia samples showed moderate to weak activity (minimum inhibitory concentration (MIC) > 625 mg/L) against the seventeen tested microbial strains (bacteria, yeasts, and dermatophytes). Overall, our study shows that A. campestris subsp. lednicensis is a promising source of bioactives with putative use as food, pharmaceutical and cosmetic ingredients.

The potential of Artemisia spp. plant extracts and endophytic bacteria to increase plant productivity: A review

“…A. campestris is a perennial plant and covered with tomentum. It does not have a noticeable scent and has upright, ovoid-shaped anthodia that are attached directly to the stem (Trifan et al, 2022). A. campestris is widely used in folk medicine due to its antioxidant, antidiabetic, nutritional, and edible properties (Zahnit et al, 2022).…”

Bio-guided isolation of a new sesquiterpene fromArtemisia cinawith anthelmintic activity againstHaemonchus contortusL3infective larvae

“…The genus Artemisia comprises approximately 500 species, distributed worldwide. Artemisia species are characterized as small herbs or shrubs with a distinctive bitter taste and a pungent aroma attributed to terpenoids, primarily monoterpenes in the essential oil, and sesquiterpene lactones [6]. They also, comprise terpenoids, flavonoids, coumarins, caffeoylquinic acids, sterols and acetylenes [7].…”

Bio-guided isolation of a new sesquiterpene from Artemisia cina with anthelmintic activity against Haemonchus contortus L3 infective larvae

“…The genus Artemisia comprises approximately 500 species distributed worldwide. Artemisia species are characterized as small herbs or shrubs with a distinctive bitter taste and an intense aroma attributed to terpenoids, primarily monoterpenes in the essential oil, and sesquiterpene lactones[ 6 ]. They also comprise terpenoids, flavonoids, coumarins, caffeoylquinic acids, sterols, and acetylenes[ 7 ].…”