Exploring the antimicrobial resistance profiles of WHO critical priority list bacterial strains

Havenga, Benjamin; Ndlovu, Thando; Clements, Tanya; Reyneke, Brandon; Waso, Monique; Khan, Wesaal

doi:10.1186/s12866-019-1687-0

Cited by 36 publications

(26 citation statements)

References 69 publications

(64 reference statements)

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“…Twenty out of thirty-six (36) P. aeruginosa presented multidrug resistance pro les and were classi ed as MDR (55.6%). [21,22]. It has been linked to outbreaks especially in ICU and onco/hematological units with reported overall incidence of carriage varying from 11.7-37.0% [23][24][25], resulting in signi cant morbidity and mortality rate close to 80% [26].…”

Section: Isolation and Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Antibiotic resistant gene of Pseudomonas aeruginosa from non-clinical environment: public health implications in Mthatha, Eastern Cape Province, South Africa

Hosu¹,

Vasaikar²,

Okuthe³

et al. 2020

Preprint

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Background Pseudomonas aeruginosa (P. aeruginosa) are Gram-negative bacilli that are ubiquitous in nature particularly plentiful in soils and in aquatic milieu because they thrive well in humid or wet environments. It has extensive metabolic versatility, ability to thrive well and colonize diverse ecological habitats such as soil, rhizosphere, wastewaters that, enhances its potential threat to public health. Abattoirs, aquatic ecosystem including generated wastewaters are latent sources of pathogenic bacteria, serve as reservoirs and contribute to the spread of antibiotic resistant genes. Several studies have focused on clinical environment while scarce data exist from non-clinical environments, which sometimes are hotspots of antibiotic resistance. Thus, the present investigation aimed to identify antibiotic resistant gene of P. aeruginosa from non-clinical sources in Mthatha, Eastern Cape and evaluate its public health implications. Results Fifty-five (55) Pseudomonas species and other organisms recovered from effluent and surface water samples included P. aeruginosa (65.4%), P. fluorescens (27.3%), Burkholderia gladioli (5.5%) and Burkholderia cepacia (1.8%). The P. aeruginosa isolates showed high resistance to aztreonam (86.1%), ceftazidime (63.9%), piperacillin (58.3%) and cefepime (55.6%); with moderate resistance displayed to imipenem (50%), piperacillin/tazobactam (47.2%), meropenem (41.7%) and levofloxacin (30.6%). Twenty out of thirty-six (36) P. aeruginosa displayed multidrug resistance profiles and were classified as multidrug resistant (MDR) (55.6%). Most of the bacterial isolates exhibited a high Multiple Antibiotic Resistance (MAR) Index of > 0.2 ranging from 0.08–0.69 with a mean MAR Index of 0.38. PCR analysis of fifteen (15) P. aeruginosa isolates detected 14 (93.3%) harbored blaSHV, 6 (40%) harbored blaTEM with the least occurring ESBL as blaCTX−M at 3 (20%). Conclusions Results of the current study indicating resistance of environmental P. aeruginosa isolates to front-line clinically relevant antipseudomonal drugs is concerning and poses risk to the environment and receiving water bodies. Given the public health relevance, the results of this study highlight the importance and urgent need of monitoring multidrug-resistant pathogens in effluent environments. These non-clinical environments are potential reservoirs of resistance genes that would further serve as avenues for the dissemination of multidrug resistant bacteria in the community.

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Section: Isolation and Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Antibiotic resistant gene of Pseudomonas aeruginosa from non-clinical environment: public health implications in Mthatha, Eastern Cape Province, South Africa

Hosu¹,

Vasaikar²,

Okuthe³

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…In addition, some AMPs can be synthesized through non-ribosomal peptide synthetases (Mukherjee et al 2011 ; Tyagi et al 2019 ). Many AMPs exhibit a broad-spectrum antibiotic activity against pathogenic bacteria (gram-negative and gram-positive), fungi, enveloped viruses, and parasites (Havenga et al 2019 ; Marcocci et al 2020 ; Zahedifard et al 2020 ). In humans and other higher organisms, AMPs are an important part of the innate immunity in response to pathogenic bacterial infections, and they are the first line of defense for various organisms against infections of multiple pathogenic microorganisms (Tang et al 2018 ; Zasloff, 2002 ).…”

Section: Introductionmentioning

confidence: 99%

Plant antimicrobial peptides: structures, functions, and applications

Jian

et al. 2021

Bot Stud

121

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Antimicrobial peptides (AMPs) are a class of short, usually positively charged polypeptides that exist in humans, animals, and plants. Considering the increasing number of drug-resistant pathogens, the antimicrobial activity of AMPs has attracted much attention. AMPs with broad-spectrum antimicrobial activity against many gram-positive bacteria, gram-negative bacteria, and fungi are an important defensive barrier against pathogens for many organisms. With continuing research, many other physiological functions of plant AMPs have been found in addition to their antimicrobial roles, such as regulating plant growth and development and treating many diseases with high efficacy. The potential applicability of plant AMPs in agricultural production, as food additives and disease treatments, has garnered much interest. This review focuses on the types of plant AMPs, their mechanisms of action, the parameters affecting the antimicrobial activities of AMPs, and their potential applications in agricultural production, the food industry, breeding industry, and medical field.

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“…The World Health Organization (WHO, Geneva, Switzerland) has declared that antibiotic resistance is one of the top ten global public health threats facing humanity. In addition to death and disability, antibiotic has a significant social and economic impact caused by prolonged illness, extended hospitalization and healthcare, the need for more expensive medicine, which weigh most heavily on lower- and middle-income countries [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Strategy to Combat Antibiotic Resistance: A Sight into the Combination of CRISPR/Cas9 and Nanoparticles

Wan

Draz

et al. 2021

Pharmaceutics

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Antibiotic resistance is a significant crisis that threatens human health and safety worldwide. There is an urgent need for new strategies to control multidrug-resistant (MDR) bacterial infections. The latest breakthrough in gene-editing tools based on CRISPR/Cas9 has potential application in combating MDR bacterial infections because of their high targeting ability to specifically disrupt the drug resistance genes that microbes use for infection or to kill the pathogen directly. Despite the potential that CRISPR/Cas9 showed, its further utilization has been hampered by undesirable delivery efficiency in vivo. Nanotechnology offers an alternative way to overcome the shortcomings of traditional delivery methods of therapeutic agents. Advances in nanotechnology can improve the efficacy and safety of CRISPR/Cas9 components by using customized nanoparticle delivery systems. The combination of CRISPR/Cas9 and nanotechnology has the potential to open new avenues in the therapy of MDR bacterial infections. This review describes the recent advances related to CRISPR/Cas9 and nanoparticles for antimicrobial therapy and gene delivery, including the improvement in the packaging and localizing efficiency of the CRISPR/Cas9 components in the NP (nanoparticle)/CRISPR system. We pay particular attention to the strengths and limitations of the nanotechnology-based CRISPR/Cas9 delivery system to fight nosocomial pathogens.We highlight the need for more scientific research to explore the combinatorial efficacy of various nanoparticles and CRISPR technology to control and prevent antimicrobial resistance.

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Exploring the antimicrobial resistance profiles of WHO critical priority list bacterial strains

Cited by 36 publications

References 69 publications

Antibiotic resistant gene of Pseudomonas aeruginosa from non-clinical environment: public health implications in Mthatha, Eastern Cape Province, South Africa

Antibiotic resistant gene of Pseudomonas aeruginosa from non-clinical environment: public health implications in Mthatha, Eastern Cape Province, South Africa

Plant antimicrobial peptides: structures, functions, and applications

Novel Strategy to Combat Antibiotic Resistance: A Sight into the Combination of CRISPR/Cas9 and Nanoparticles

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