Exploring<scp>ComQXPA</scp>quorum‐sensing diversity and biocontrol potential of<scp><i>B</i></scp><i>acillus</i>spp. isolates from tomato rhizoplane

Oslizlo, Anna; Štefanič, Polonca; Vatovec, S.; Glaser, Sara Beigot; Rupnik, Maja; Mandić-Mulec, Ines

doi:10.1111/1751-7915.12258

Cited by 39 publications

(42 citation statements)

References 75 publications

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“…Similarly, Bais et al (99) suggested that biofilm formation and the production of the lipopeptide compound surfactin by B. subtilis on Arabidopsis roots is essential for the plant protection activity of this organism against Pseudomonas syringae. Recently, surfactin production by Bacillus biofilms was directly imaged on plant roots by matrixassisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) (100) and showed that B. subtilis strains that originate from the same plant root can dramatically differ in surfactin production, but less so in their ability to form biofilms (101). However, biofilm strength and thickness depends also on the composition of extracellular matrix components and these change in relation to available nutrients (102).…”

Section: Bacillaceae As Plant-beneficial Rhizobacteriamentioning

confidence: 99%

Ecology of Bacillaceae

2015

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Members of the family Bacillaceae are among the most robust bacteria on Earth, which is mainly due to their ability to form resistant endospores. This trait is believed to be the key factor determining the ecology of these bacteria. However, they also perform fundamental roles in soil ecology (i.e., the cycling of organic matter) and in plant health and growth stimulation (e.g., via suppression of plant pathogens and phosphate solubilization). In this review, we describe the high functional and genetic diversity that is found within the Bacillaceae (a family of low-G+C% Gram-positive spore-forming bacteria), their roles in ecology and in applied sciences related to agriculture. We then pose questions with respect to their ecological behavior, zooming in on the intricate social behavior that is becoming increasingly well characterized for some members of Bacillaceae. Such social behavior, which includes cell-to-cell signaling via quorum sensing or other mechanisms (e.g., the production of extracellular hydrolytic enzymes, toxins, antibiotics and/or surfactants) is a key determinant of their lifestyle and is also believed to drive diversification processes. It is only with a deeper understanding of cell-to-cell interactions that we will be able to understand the ecological and diversification processes of natural populations within the family Bacillaceae. Ultimately, the resulting improvements in understanding will benefit practical efforts to apply representatives of these bacteria in promoting plant growth as well as biological control of plant pathogens.

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Section: Bacillaceae As Plant-beneficial Rhizobacteriamentioning

confidence: 99%

Ecology of Bacillaceae

2015

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“…Several isolates of Bacillus , especially Bacillus subtilis directly affect plant growth promotion, by the mechanisms such as acting as nitrogen fixers, as well as producers of indol butyric acid (IBA) (Araujo, Henning, & Hungria, ), indol acetic acid (IAA), and siderophores (Puente, Bashan, Li, & Lebsky, ), in addition to biocontrol activity (Ali, Charles, & Glick, ; Hanif et al., ; Oslizlo et al., ) and activities of protein expression relevant to the pharmaceutical and industrial sector (Guan et al., ). Bacillus subtilis has such varied functional capacities, which suggests that it has a versatile potential to be used as a plant growth promoter.…”

Section: Introductionmentioning

confidence: 99%

Sugarcane growth and nutrition levels are differentially affected by the application of PGPR and cane waste

Santos

Kandasamy²,

Rigobelo

2018

MicrobiologyOpen

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Mineral and organic fertilization can be optimized by using rhizobacteria which increases dry matter, yield, and nutrients in the soil and plant, among the other biological inputs. However, the discovery of single microbes or a consortium that can benefit plants has been a challenge. In this context, this study aimed to evaluate the effects of Bacillus subtilis and Bacillus pumilus combined with mineral fertilization and sugar and alcohol industry by-products in presprouted and the initial growth phase of sugar cane seedlings. The study was carried out in two phases. Phase 1 included presprouted seedlings with T1 = untreated control, T2 = B. subtilis, T3 = B. pumilus, and T4 = B. subtilis + B. pumilus treatments. Phase 2 included the same treatments with four types of fertilization: F1 = mineral fertilization, F2 = mineral fertilization + vinasse, F3 = mineral fertilization + filter cake, and F4 = mineral fertilization + filter cake compost. Of the phase 1 treatments, T2 (B. subtilis) was the best promoter of root growth and the total dry matter compared to the control with an increase of 23.0% compared to the control. In phase 2, B. pumilus application, increased the total dry matter by 13%, the number of tillers by 37%, and the diameter of the tillers by 48% when combined with mineral fertilization. The combined application of B. subtilis and B. pumilus increased the phosphorus content by 13% in soil treated with mineral fertilization and filter cake compost. The results of the this study strongly suggest that the use of B. subtilis and B. pumilus together with these by-products can improve soil fertility parameters and decrease adverse effects associated with vinasse fertilization, in addition to providing shoot and root growth and providing collective synergy for a high yield of sugarcane production with environmental benefits.

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“…Integration of P epsA - gfp reporter fusion into different strains was performed by transformation with YC164 genomic DNA (41) (Table 1). Integration of srfA::Tn917 (mls) mutation into different strains was performed by transformation with BM1044 genomic DNA (74) (Table 1). Strains with P tapA - yfp reporter fusion were constructed by transformation with BM1115 genomic DNA (75).…”

Section: Methodsmentioning

confidence: 99%

Quorum sensing inBacillus subtilisslows down biofilm formation by enabling sporulation bet hedging

Špacapan

Danevčič

Štefanič

et al. 2019

Preprint

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7 8 Running Head: Quorum sensing contributes to bet hedging in Bacillus 9 #Address correspondence to Ines Mandic-Mulec, ines.mandicmulec@bf.uni-lj.si. 10 42Biofilms are multicellular groups encased in extracellular matrix that are believed to be the 43 default mode of microbial growth in nature (1-3). Biofilm formation is often regulated by 44 quorum sensing (QS) (4), a wide spread microbial communication system that coordinates 45 bacterial gene expression in accordance with cell density (5). Bacillus subtilis is the most 46 studied species in the genus Bacillus (6, 7), which has served over the years as an excellent 47 model to investigate development of metabolically dormant and heat resistant spores (8), a 48 model for biofilm development (9-12), division of labor (13-16) and for a variety of intra 49 and interspecies social interactions (17). This Gram-positive spore former relies on peptide 50 based QS system, encoded by the comQXPA gene cluster (18, 19), which is wide spread 51 among Firmicutes (20). It controls transcriptional activity of several adaptive processes 52 including synthesis of a lipopetide antibiotic surfactin (21-23), exoprotease production (24, 53 25), and competence development (26). The extracellular signaling peptide ComX is 54 synthesized as a pre-peptide, cleaved and post-translationally modified by the isoprenoid 55 transferase ComQ (21, 27). Therefore, the gene comQ is essential for a functional and active 56 QS system. Mature and biologically active ComX accumulates extracellularly, where it 57interacts with the sensory histidine kinase ComP, which then phosphorylates ComA (28). 58ComA-P in its active phosphorylated form regulates transcription of several target genes (29-59 31). ComA-P also increases the transcription rates of the pleiotropic regulatory gene degQ 60 (25, 30, 32), which ultimately increases the DegU phosphorylation rate (25). DegU-P is 61 required for the activation of extracellular degradative enzyme production. This regulatory 62 network is relevant also during biofilm development (33), where operational ComX 63 dependent QS is required for exoprotease production (24). 64In biofilms, cells are encased in a structure formed out of extracellular polysaccharides (Eps), 65 proteins, and extracellular DNA (3); but the ratios of each biofilm constituent differ 66 depending on the specific strain, media and growth conditions (34). In B. subtilis the epsA-O 67 operon is involved in the production of the major polysaccharide component of the biofilm 68 matrix (35), which is essential for development of floating biofilm (pellicle) (36). TasA, the 69 major matrix protein, encoded by the tapA-tasA-sipW operon (hereafter referred to as the 70 tapA operon), gives structural support to floating biofilm. Although TasA and TapA proteins 71 are not essential for the formation of floating biofilms, the tasA mutant forms a less 72 prominent structures (37, 38). The molecular regulation of the operons involved in the 73 synthesis of the biofilm matrix components is very complex (39)....

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ExploringComQXPAquorum‐sensing diversity and biocontrol potential ofBacillusspp. isolates from tomato rhizoplane

Cited by 39 publications

References 75 publications

Ecology of Bacillaceae

Ecology of Bacillaceae

Sugarcane growth and nutrition levels are differentially affected by the application of PGPR and cane waste

Quorum sensing inBacillus subtilisslows down biofilm formation by enabling sporulation bet hedging

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