Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced microfocus computed tomography of biological tissues

Bournonville, Sébastien de; Vangrunderbeeck, Sarah; Ly, Hong Giang T.; Geeroms, Carla; Borggraeve, Wim M. De; Parac-Vogt, Tatjana; Kerckhofs, Greet

doi:10.1016/j.actbio.2020.01.038

Cited by 28 publications

(40 citation statements)

References 45 publications

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“…However, the sample often needs to be dehydrated during the preparation. Our own data shows that one of the solutions to avoid tissue shrinkage is the use of CE-CT with a less destructive CESA, such as Hafnium-substituted Wells-Dawson polyoxometalate (Hf-WD POM) [78,79]. Figure 2 illustrates the application of the successive staining of Hf-WD POM (Figure 2A-E,K) and isotonic Lugol's iodine (Figure 2 F-J,L) on a mouse heart.…”

Section: Anatomical and Morphometric Assessment Of The Whole Heartmentioning

confidence: 99%

“…They found that aging and the HFD had a significant impact on the 3D spatial organization and branching of the vasculature, something that is difficult to obtain via standard 2D histology. More recently, de Bournonville and colleagues showed that Monolacunary WD-POM (Mono-WD-POM) also allows to visualize bone marrow and kidney blood vessels with a lower synthesis time at lower costs [79].…”

Section: Spatial Distribution and Morphometrics Of The Vascular Treementioning

confidence: 99%

“…They found that aging an the HFD had a significant impact on the 3D spatial organization and branching of th vasculature, something that is difficult to obtain via standard 2D histology. More recentl de Bournonville and colleagues showed that Monolacunary WD-POM (Mono-WD-POM also allows to visualize bone marrow and kidney blood vessels with a lower synthes time at lower costs [79]. Finally, the brain is an organ that is less commonly studied with CE-CT imagin High variations in the extent of CCA filling are often seen due to the complexity of i vascular network, conferring different images of the vasculature for different subjects.…”

Section: Spatial Distribution and Morphometrics Of The Vascular Treementioning

confidence: 99%

“…A higher contrast was obtained with PTA and PMA compared with Lugol's iodine. However, as PTA, PMA and Lugol's iodine are known to cause tissue shrinkage due to their high acidity, we used the previously mentioned nondestructive Hf-WD POM [79] on a fixed abdominal rat aorta. Using this CESA enabled us to distinguish, in 3D, the medial and adventitial layers and to segment the elastic fibers in the media without affecting the original aortic structure (Figure 7).…”

Section: Microstructure Of the Vessel Wallmentioning

confidence: 99%

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A Review of Ex Vivo X-ray Microfocus Computed Tomography-Based Characterization of the Cardiovascular System

Leyssens

Pestiaux

Kerckhofs

2021

IJMS

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Cardiovascular malformations and diseases are common but complex and often not yet fully understood. To better understand the effects of structural and microstructural changes of the heart and the vasculature on their proper functioning, a detailed characterization of the microstructure is crucial. In vivo imaging approaches are noninvasive and allow visualizing the heart and the vasculature in 3D. However, their spatial image resolution is often too limited for microstructural analyses, and hence, ex vivo imaging is preferred for this purpose. Ex vivo X-ray microfocus computed tomography (microCT) is a rapidly emerging high-resolution 3D structural imaging technique often used for the assessment of calcified tissues. Contrast-enhanced microCT (CE-CT) or phase-contrast microCT (PC-CT) improve this technique by additionally allowing the distinction of different low X-ray-absorbing soft tissues. In this review, we present the strengths of ex vivo microCT, CE-CT and PC-CT for quantitative 3D imaging of the structure and/or microstructure of the heart, the vasculature and their substructures in healthy and diseased state. We also discuss their current limitations, mainly with regard to the contrasting methods and the tissue preparation.

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Section: Anatomical and Morphometric Assessment Of The Whole Heartmentioning

confidence: 99%

Section: Spatial Distribution and Morphometrics Of The Vascular Treementioning

confidence: 99%

Section: Spatial Distribution and Morphometrics Of The Vascular Treementioning

confidence: 99%

Section: Microstructure Of the Vessel Wallmentioning

confidence: 99%

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A Review of Ex Vivo X-ray Microfocus Computed Tomography-Based Characterization of the Cardiovascular System

Leyssens

Pestiaux

Kerckhofs

2021

IJMS

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“…Lastly, similar to conventional histopathology, 317 shrinkage of tissue during formalin fixation and staining is widely known and may influence volume 318 calculations. However, this could be mitigated by use of polyoxometalates 41 . 319…”

Section: Tb Lesion Formation Through Bronchial Obstruction 203mentioning

confidence: 99%

3D microarchitecture of the human tuberculous granuloma

Wells

Glasgow

Nargan

et al. 2020

Preprint

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20 21 throughout the 1940s 1 and 1950s 2 when post-mortem and resected human lung tissues were 55 routinely available. These pioneering studies have strongly influenced our current understanding 56 of the spectrum of tuberculous lesions, morphology and pathology. 57The prevailing dogma in the TB field is that TB granulomas form spherical or ovoid 58 structures within the parenchyma 3-11 . However, this assumption is not always supported by 59 experimental evidence. Further, remarkably little is known about the structure of the caseous 60 granuloma, the distinctive feature of infection by Mtb in humans. Hence, a deeper understanding 61 of the human TB granuloma is urgently needed to more accurately inform preventive and 62 therapeutic TB strategies. 63The ability to examine pathological TB structures within large tissues in 3D could allow 64 identification of disease-specific features and improve diagnosis. Hence, it is reasonable to 65 speculate that the limitations of conventional histological analysis have begun to hinder more 66 detailed examination of human TB pathophysiology in the current antibiotic era, especially with the 67 emergence of HIV. Other factors contributing to our limited understanding are the reliance on 68 animal models which do not recapitulate human pulmonary TB phenotypes, and the paucity of 69 routinely available resected human TB lung tissues 12,13 . Therefore, imaging approaches that 70 provide high-resolution digital 3D imaging of TB lesions will allow comprehensive analysis of the 71 complex 3D microanatomical features specific to pulmonary TB. 72 X-ray computed tomography (CT) is an invaluable imaging tool for nondestructive 73 assessment of tissue in medical diagnosis [14][15][16] . High resolution micro-CT (µCT) is typically used 74 for materials with high electron density and lends itself to ex vivo analysis of pathologies involving 75 bone structure or calcium deposition 17 . Imaging of soft tissue can be improved by addition of 76 electron-dense contrast agents (e.g., iodine, osmium, or tungsten) or using high-energy flux 77 monochromatic x-rays generated by synchrotrons. To our knowledge, however, no study has 78 reported the use of µCT to examine bacterial or viral disease in human lungs. While µCT is an 79 experimental imaging modality, high-resolution computed X-ray tomography (HRCT) is often used While conventional histopathological analysis provides detailed information on very small areas of 130 interest, it cannot contextualize TB lesions within the overall lung architecture. This has limited our 131 understanding of the distribution and shapes of lesions within the human TB lung. µCT has the 132 potential to improve our understanding of the evolution of granuloma formation and structure, 133 relative to the diffusion of drugs, O 2 and nutrients. To contextualize TB lesions relative to the 134 vasculature and airways, we used µCT to scan a large slice (~14 x 1.5 x 6 cm) of infected lung at 135 52.0 µm resolution (Figure 2A, Figure S1, Video S1). 3D segmentation shows th...

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