Exploring platelet lysate hydrogel-coated suture threads as biofunctional composite living fibers for cell delivery in tissue repair

Abstract

Special issue 'Advances in biomaterials for orthopaedic applications' Exploring platelet lysate hydrogel-coated suture threads as biofunctional composite living fibers for cell delivery in tissue repair

Perfusion Flow Enhances Viability and Migratory Phenotype in 3D-Cultured Breast Cancer Cells

“…This quantity, that varies between 0 and 1, captures how much a given shape differs from a circle (eccentricity=0) and is equivalent to the aspect ratio used in Ref. 16 to quantify actin fibers alignment.…”

“…This is equivalent to the aspect ratio used in Ref. 16 to quantify actin fiber alignment and was computed for each cell using the following equation, where a and b represent the semi-major and semi-minor axes of each cell, respectively. …”

Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model

“…3D-printed molds with four misaligned fiber replicates placed in 6-well plates were sterilized in 70% ( v / v ) ethanol for 30 min followed by 30 min of ultraviolet radiation. Afterward, isotropic threads were immersed in a solution of 10 U/mL thrombin from bovine plasma (40–30 NIH units/mg protein, Sigma-Aldrich, Saint Louis, MO, USA) in 5 mM calcium chloride (CaCl 2 , MERCK KGaA, Darmstadt, Germany) for 45 min at RT [ 38 , 71 ]. Then, threads were transferred to a new channel and coated with a solution of hTDCs in PL hydrogel, produced as described previously [ 71 , 72 ], at a density of 2.5 × 10 5 cells/mL for 2 h at 37 °C in a 5% humidity atmosphere for PL gelation.…”

Mechanical properties of cell- and microgel bead-laden oxidized alginate-gelatin hydrogels

“…Synthetic hydrogels such as polyethylene glycol (PEG), 3 2-hydroxyethyl methacrylate (HEMA)-based hydrogels, 4 poly(oxazoline)s (POx), 5 or polyacrylamide (PAAm), as well as naturally derived hydrogels such as alginate, gelatin, collagen, or fibrin matrices, [6][7][8][9] have been used in various biomedical appli-cations. 1 Frequently, these include the delivery of cells, [10][11][12][13][14] growth factors, [15][16][17] or drugs, [18][19][20][21][22] in vitro tissue and tumor models, [23][24][25] and tissue engineering (TE) matrices. 2,[26][27][28][29][30] The role of biopolymer hydrogels in TE is to chemically and physically mimic the extracellular matrix (ECM) environment to facilitate controlled cell adhesion, proliferation, differentiation, and matrix remodeling.…”