Exploring Packaged Microvesicle Proteome Composition of Chinese Hamster Ovary Secretome

doi:10.4172/2155-9821.1000274

Cited by 3 publications

(2 citation statements)

References 44 publications

(54 reference statements)

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“…We refer readers to the many profiling studies which have been published on this subject for further information. 36,66,[94][95][96][97][98][99][100] Prototypical exosome markers include tetraspannins such as CD9, CD63 and CD81, and ESCRT proteins Alix and TSG101. 37,101 Unique and universal MV markers are somewhat less well-defined.…”

Section: Functional Cargoes In Microvesicles Derived From Tumor Cellsmentioning

confidence: 99%

The biology of extracellular microvesicles

Sedgwick

D’Souza‐Schorey

2018

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The study of extracellular vesicles (EVs) is a rapidly evolving field, owing in large part to recent advances in the realization of their significant contributions to normal physiology and disease. Once discredited as cell debris, these membrane vesicles have now emerged as mediators of intercellular communication by interaction with target cells, drug and gene delivery, and as potentially versatile platforms of clinical biomarkers as a result of their distinctive protein, nucleic acid and lipid cargoes. While there are multiple classes of EVs released from almost all cell types, here we focus primarily on the biogenesis, fate and functional cargoes of microvesicles (MVs). MVs regulate many important cellular processes including facilitating cell invasion, cell growth, evasion of immune response, stimulating angiogenesis, drug resistance and many others.

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Section: Functional Cargoes In Microvesicles Derived From Tumor Cellsmentioning

confidence: 99%

The biology of extracellular microvesicles

Sedgwick

D’Souza‐Schorey

2018

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162

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“…The nature of that cargo, which has been investigated by several groups (Belliveau & Papoutsakis, 2023; Busch et al, 2022; Keysberg et al, 2021), is now known to vary significantly as a function of metabolite (ammonia and osmotic) stress (Belliveau & Papoutsakis, 2023). Preliminary proteomic analysis has also been performed on CHO EVs, with large and diverse protein populations observed in both large and small CHO EVs from various phases of culture (Keysberg et al, 2021; Kumar et al, 2016). Still, nothing is known about the specific function of CHO EV cargo in culture.…”

Section: Introductionmentioning

confidence: 99%

Kinetic and functional analysis of abundant microRNAs in extracellular vesicles from normal and stressed cultures of Chinese hamster ovary cells

Belliveau,

Thompson,

Papoutsakis

2023

Biotech & Bioengineering

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Chinese hamster ovary (CHO) cells release and exchange large quantities of extracellular vesicles (EVs). EVs are highly enriched in microRNAs (miRs, or miRNAs), which are responsible for most of their biological effects. We have recently shown that the miR content of CHO EVs varies significantly under culture stress conditions. Here, we provide a novel stoichiometric (“per‐EV”) quantification of miR and protein levels in large CHO EVs produced under ammonia, lactate, osmotic, and age‐related stress. Each stress resulted in distinct EV miR levels, with selective miR loading by parent cells. Our data provide a proof of concept for the use of CHO EV cargo as a diagnostic tool for identifying culture stress. We also tested the impact of three select miRs (let‐7a, miR‐21, and miR‐92a) on CHO cell growth and viability. Let‐7a—abundant in CHO EVs from stressed cultures—reduced CHO cell viability, while miR‐92a—abundant in CHO EVs from unstressed cultures—promoted cell survival. Overexpression of miR‐21 had a slight detrimental impact on CHO cell growth and viability during late exponential‐phase culture, an unexpected result based on the reported antiapoptotic role of miR‐21 in other mammalian cell lines. These findings provide novel relationships between CHO EV cargo and cell phenotype, suggesting that CHO EVs may exert both pro‐ and antiapoptotic effects on target cells, depending on the conditions under which they were produced.

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