Exploring omega‐3 fatty acids, enzymes and biodiesel producing thraustochytrids from Australian and Indian marine biodiversity

Gupta, Adarsha; Singh, Deepti; Byreddy, Avinesh R.; Thyagarajan, T. R.; Sonkar, Shailendra P.; Mathur, Anshu S.; Tuli, Deepak K.; Barrow, Colin J.; Puri, Munish

doi:10.1002/biot.201500279

Cited by 54 publications

(39 citation statements)

References 50 publications

(60 reference statements)

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“…Enzymes, antimicrobials, and biopesticides have been isolated from mangrove fungi (Cheng et al 2009). Long-chain omega-3 fatty acids producing microalgae have also been found in mangrove forests (Gupta et al 2016b). Anyhow, it is strongly emphasized that any mangrove-based lignocellulosic biomass exploitation must be only applied in mangrove ecosystems where conservation efforts are well implemented, or refrain otherwise.…”

Section: Determination Of Klason Lignin and Oligosaccharides In Biomassmentioning

confidence: 99%

Avicennia marina biomass characterization towards bioproducts

et al. 2017

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Avicennia marina is the only naturally occurring mangrove specie in the arid Arabian Gulf coast of the United Arab Emirates (UAE). Due to the water scarcity of this arid-region, A. marina is a precious biomass resource for the UAE that does not require fresh water for growing, and is able to grow in the Arabian Gulf high salinity conditions, over 40g/kg. This non-fresh water lignocellulosic arid-region bioresource may be used for the production of high valuable chemicals. The objective of the present manuscript is to characterize the lignocellulosic composition of Arabian Gulf A. marina, as a first attempt to highlight its importance in a biobased economy in arid regions. Avicennia marina stem, leaves and pneumatophors samples were collected from two locations in the United Arab Emirates. A. marina samples were chemically characterized for sugar composition, ash content and byproducts using standardized protocols. The analysis revealed that A. marina arabinan, xylan, glucan and lignin composition ranges, in g/100g_TS (TS: total solids), between 1-22, 5-18, 10-31, and 21-48, respectively. The highest composition of xylan and glucan (g/100g_TS) was obtained for stems and pneumatophors, 45 and 38, respectively. Xylan and glucan are the polymeric precursors for the production of high value chemicals, e.g. furfural and hydroxymethylfurfural (HMF), respectively. Under the characterization conditions, it was obtained furfural and HMF (g/100g_TS) in the ranges of 0.05-0.42, and 0.45-2.1, respectively.

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Section: Determination Of Klason Lignin and Oligosaccharides In Biomassmentioning

confidence: 99%

Avicennia marina biomass characterization towards bioproducts

et al. 2017

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“…For the process to become effective, it requires a lipase with excellent fatty acid selectivity. Efforts to isolate a fatty acid-selective lipase or to improve the natural lipases for fatty acid selectivity have failed to provide fruitful results [20,21]. In this work, we took a lipase from Geobacillus thermoleaverans (GTL) as a model protein and engineered it to enhance its ω-3 FAs selectivity using semirational and rational approaches.…”

Section: Introductionmentioning

confidence: 99%

Substrate structure and computation guided engineering of a lipase for omega-3 fatty acid selectivity

Moharana

Rao

2020

PLoS ONE

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Enrichment of omega-3 fatty acids (ω-3 FAs) in natural oils is important to realize their health benefits. Lipases are promising catalysts to perform this enrichment, however, fatty acid specificity of lipases is poor. We attempted to improve the fatty acid selectivity of a lipase from Geobacillus thermoleovorans (GTL) by two approaches. In a semi-rational approach, amino acid positions critical for binding were identified by docking the substrate to the GTL and best substitutes at these positions were identified by site saturation mutagenesis followed by screening to obtain a variant of GTL (CM-GTL). In the second approach based on rational design, a variant of GTL was designed (DM-GTL) wherein the active site was narrowed by incorporating two heavier amino acids in the lining of acyl-binding pocket to hinder access to bulky ω-3 FAs. The affinities DM-GTL with designed substrates were evaluated in silico. Both, CM-GTL and DM-GTL have shown excellent ability to discriminate against the ω-3 FAs during hydrolysis of oils. Engineering the binding pocket of an enzyme of a complex substrate, such as a triglyceride, by incorporating the information on substrate structure and computationally derived binding modes, has resulted in designing two efficient lipase variants with improved substrate selectivity.

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“…For the process to become effective, it requires a lipase with excellent fatty acid selectivity. Efforts to isolate a fatty acid-selective lipase or to improve the natural lipases for fatty acid selectivity have failed to provide fruitful results [20,21]. In this work, we took a lipase from Geobacillus thermoleaverans (GTL) as a model protein and engineered it to enhance its fatty acid selectivity using semi-rational and rational approaches.…”

Section: Introductionmentioning

confidence: 99%

Substrate structure and computation guided engineering of a Lipase for Omega-3 fatty acid selectivity

Moharana

Rao

2019

Preprint

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AbstractOptimum health benefits of omega-3 fatty acids (ω-3 FAs) require it to be concentrated in its natural sources. Fatty acid selectivity of lipase governs the efficacy of the production of lipase-mediated ω-3 FAs concentrates. We attempted to improve the fatty acid selectivity of a lipase from thermophilic bacteriumGeobacillus thermoleovorans(GTL) by two approaches. In a semi-rational approach, six amino acid positions of GTL interacting with the substrate, were identified by docking and were subjected to site-saturation mutagenesis. Three best substitutions were incorporated into GTL(CM-GTL). Hydrolysis of oil by lipase was monitored in a pH-Stat and the fatty acids released at various time points were analyzed by GC-MS.CM-GTL showed a significant improvement in discrimination against DHA during hydrolysis. In the second approach based on rational design, the active site was narrowed by incorporating heavier amino acids in the lining of acyl-binding pocket to hinder access to bulky ω-3 FAs. For this purpose, two amino acids surrounding the opening of the acyl pocket were replaced with the next heavier amino acids and the affinities were evaluatedin silico.The double mutant, thus deigned, was found to be excellent in discriminating the ω-3 FAs during hydrolysis of triglycerides. Engineering the binding pocket of a complex substrate, such as a triglyceride, with the supportive information on substrate structure and its binding modes with the enzyme provided by computational methods, has resulted in designing two efficient lipase variants with improved substrate selectivity.

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Exploring omega‐3 fatty acids, enzymes and biodiesel producing thraustochytrids from Australian and Indian marine biodiversity

Cited by 54 publications

References 50 publications

Avicennia marina biomass characterization towards bioproducts

Avicennia marina biomass characterization towards bioproducts

Substrate structure and computation guided engineering of a lipase for omega-3 fatty acid selectivity

Substrate structure and computation guided engineering of a Lipase for Omega-3 fatty acid selectivity

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