Exploring of bioactive compounds in essential oil acquired from the stem and root derivatives of Hypericum triquetrifolium callus cultures

Abstract

The chemical profile of the essential oil of callus and cell suspension cultures derivatives from stem and root of Hypericum triquetrifolium were explored by ITEX/GC-MS. The major constituents for stem derivatives were undecane (78.44%) and 2,4,6-trimethyl-octane (9.74%) for fresh calli, 2,4-dimethyl-benzaldehyde (46.94%), 2,3-dimethyl-undecane (28.39%), 2,4-dimethyl-1-hexene (10.17%), 1,2-oxolinalool (3.64%) and limonene (3.55%) for dry calli and undecane (61.24%), octane, 2,4,6-trimethyl- (16.73%), nonane, 3-methyl-(3.74%), 2,5-diphenyl-benzoquinone (3.70%) and limonene (3.60%) for cell suspension. However, for root derivatives, the dominated components were: undecane (49.94%), eucalyptol (12.07%), limonene (9.98%), toluene (9.03%) and 3-methyl-nonane (4.29%) for fresh calli, 2,4-dimethyl-benzaldehyde (29.80%), 1,1-dimethylethyl-cyclohexane (14.99%), 3-methyl-pentanal (14.99%), undecane (10.04%), beta-terpinyl acetate (8.60%), 1,2-oxolinalool (6.27%) and 2-pentyl-furan (4.09%) for dry calli, undecane (52.38%), 2,4,6-trimethyl-octane (13.81%), 3-methyl-nonane (5.73%), toluene (4.82%) and limonene (4.57%) for cell suspension derivative in root. The attained outcomes indicated that the alkane, aldehyde and monoterpene fractions dominated the chemical composition of essential oils.

Hypericum Essential Oils—Composition and Bioactivities: An Update (2012–2022)

“…Moreover, other biotechnological tools have been applied, such as callus culture, which produces biomass on a large scale and could be used as a good experimental system for further research on essential oil production. Calli essential oil cultivation enabled the selection of a desired compound or group of compounds with specific aromas or activities as a response to chemical elicitors that stimulate biotic and abiotic stress in vitro [25,26]. Nevertheless, Gas Chromatography (GC) is, by all means, the 'golden standard method' in the chemical analysis of EOs, especially enforced with the aid of GC-MS (Mass Spectrometry) and GC-FID (Flame Ionization Detector) for both the identification and quantification of the content as well as the composition variations, regardless of the extraction protocol [17,[22][23][24].…”

“…Much diversity has been reported for this plant when collected from different populations in Tunisia [45], while the oils from Iran and Turkey were abundant in germacrene-D and hexanal, respectively [29,50]. Tahir et al [25] characterized the EO from H. triquetrifolium cultures produced by the root and stem for the first time and cited that alkane, aldehyde, and monoterpene compounds are the foremost fractions. Furthermore, H. thymopsis and H. scabroides showed different results when collected from other locations in Turkey [27].…”

Extraction of Cuminum cyminum and Foeniculum vulgare Essential Oils and Their Antibacterial and Antibiofilm Activity against Clinically Isolated Porphyromonas gingivalis and Prevotella intermedia: An In Vitro Study

“…The EOs were extracted via the hydrodistillation method using the Clevenger apparatus under optimal operating conditions according to the standard protocol [15]. Freshly ground samples were added to sterilized distilled water in a 500 mL round-bottom flask and boiled (balloon heating mantle, Fibroman HT-W, Zhengzhou, China) continuously for 3 h. The EO was collected, dried under anhydrous sodium sulfate, and stored in a sealed amber vial at 4 • C until used.…”

Assessment of Phytochemical Content and Antioxidant Activity of the Extracts Collected from the Leaves and Flowers of Hypericum scabrum

“…The sample was recorded by a voucher number (KA-1) and stored at the herbarium of the college of Agricultural Sciences. The specimens were dried in the shade at 24º C for 7 days, and they were grounded (Tahir et al, 2017). The extracts were collected by soaking in solvents including distilled water and pure ethanol.…”