Exploring new complex protein supplement solutions for clinical embryo culture media

Abstract

RESULTS:The intentional destruction of all external drilled dewar tanks created an aspiration noise as room air initially warmed the interstitial space outside the inner tank. Conversely, internal drilled tanks displayed overt bubbling of its inner liquid chamber and immediate LN vaporization (within 10 sec). LN vapor streaming outside the cap and neck of the external drilled tanks was also evident within 30 sec. An external thermocouple registered 5oC within 3 min. Ice was seen on the cap surface by 3 min, while gradual frost build-up occurred over several hours. Icing and condensation on the tank walls was apparent early on and throughout failure. A 20% evaporation detected by weight took about 4h, while the first internal temperature alarm at -194oC did not occur until 5.5-6.5h. The ID tanks reached -170oC sooner (+14-15h) with 65-75% evaporation compared to >95% evaporation for the ED group (+18-19h), prior to the rapid rise of temperature for both treatments (subzero by +24-25h). The ED-treated 73L tank responded similar to the 35HC tanks, but at twice the evaporation rate. The external drill size did not affect evaporation rates.CONCLUSIONS: Tank quality and type of vacuum breach can influence the rate of failure. In all cases overt physical signs of pending failure were continuously visible for >14h before critical temperatures were reached. Overall, external quality measurements and device systems represent a promising future offering greater precision, labor efficiency, and improved specimen security/ safety.SUPPORT: None