Exploring Mechanism of Key Chinese Herbal Medicine on Breast Cancer by Data Mining and Network Pharmacology Methods

Yang, Lina; Wu, Zhulin; Yang, Zhenjiang; Li, Shiguang; Ouyang, Chensheng

doi:10.1007/s11655-020-3422-y

Cited by 20 publications

(9 citation statements)

References 47 publications

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“…BC is a common malignant tumor in women, and its exact pathogenesis has not been clarified yet. TCM believes that deficiency of vital qi and deficiency of blood is the internal cause and root of BC [ 18 ]. “Vital qi” in TCM is the immune function of modern medicine.…”

Section: Discussionmentioning

confidence: 99%

Effects of Chinese Herbal Formula on Immune Function and Nutritional Status of Breast Cancer Patients

Liu

2022

Computational and Mathematical Methods in Medicine

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Background. Chinese herbal formulas have certain effects on patients with breast cancer (BC). This article discussed the effect of Buqi Yangxue decoction on the immune function and nutritional status of BC patients and provided an evidence for traditional Chinese medicine (TCM) to improve the quality of life and curative effect of BC patients. Methods. 66 cases of BC patients were divided into control group (n =33) and Chinese herbal formula group (n =33). The control group was received with TE chemotherapy, and the Chinese herbal formula group was received with Buqi Yangxue decoction combined with TE chemotherapy. Nutritional status, immune function, TCM symptom quantitative score, and adverse reactions were compared between the two groups. Results. There was no difference in all indexes between the two groups before intervention. After 4 weeks, the nutritional indexes ALB, PA, TRF, and TP in Chinese herbal formula group were higher than those in control group, except HGb. CD3+, CD4+, and CD4+/CD8+ in both groups were sharply higher than before treatment, while CD8+ was dramatically lower, and the changes in Chinese herbal formula group were more obvious than those in control group. In Chinese herbal formula group, the levels of IgG, IgA, and IgM were sharply increased compared with control group. The TCM syndrome scores in both groups were decreased significantly after treatment, especially in Chinese herbal formula group. In addition, nausea and vomiting, inappetence, liver function impairment, leukopenia, and thrombocytopenia occurred in both groups. There was no clear difference in the incidence of adverse reactions between Chinese herbal formula group and control group. Conclusion. Buqi Yangxue decoction can effectively improve the nutritional status and immune function of BC patients, which has important clinical significance for the later comprehensive treatment.

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Section: Discussionmentioning

confidence: 99%

Effects of Chinese Herbal Formula on Immune Function and Nutritional Status of Breast Cancer Patients

Liu

2022

Computational and Mathematical Methods in Medicine

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“…3A and B). According to the PPI data, the common target genes TP53, CCND1, CASP3, AKT1, IL4, CXCL8, IL6, IL10, KDR, PPARG, BCL2, PCNA, and EPHB2 might be potential target genes for SKN in the treatment of CRC [4].…”

Section: Ppi Networkmentioning

confidence: 99%

“…However, due to the complicated components of TCM, its underlying antitumor mechanisms are not completely understood. Currently, an increasing number of researchers have realized that network pharmacology can provide an effective approach to identify the association between targets, diseases, and drugs and reveal the mechanisms of TCM from a holistic view [4]. In addition, increasing evidence has shown that many drugs can achieve their therapeutic activities by regulating multiple targets.…”

Section: Introductionmentioning

confidence: 99%

Network Pharmacology-Based Prediction and Verification of the Mechanism of Shikonin in the Treatment of Colorectal Cancer

Wang

Cui

Shi

et al. 2021

Preprint

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Background: Zicao is the dried root of Lithospermum erythrorhizon Sieb, et Zucc, Arnebia euchroma (Royle) Johnst, or Arnebia guttata Bunge and is commonly used to treat viral infection, inflammation, arthritis, and cancer in traditional Chinese medicine. Shikonin (SKN), a naturally occurring naphthoquinone, is a major active chemical component isolated from Zicao and exhibits anticancer activity according to previous research. However, the underlying mechanism has not been elucidated, so further research is necessary to verify its traditional application. The purpose of the present study was to investigate the antitumor mechanism of SKN in colorectal cancer (CRC) through network pharmacology and experiments.Methods: The SymMap database and GeneCards were adopted to predict the potential targets of SKN and CRC, while cotargets were obtained via a Venn diagram. The cotargets were imported from the String and DAVID websites, the protein-protein interaction (PPI) network was constructed, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed. With regard to the prediction of KEGG by DAVID, the compound-target-pathway network was generated by connecting potential pathways with the corresponding targets. According to the network pharmacological analysis, cytological experiments, real-time PCR (RT-PCR), and Western blot (WB) were used to verify the key signaling pathway.Results: According to the network pharmacological analysis, the most relevant target of SKN to the treatment of colorectal cancer was IL6. GO and KEGG enrichment analyses indicated that various kinases and the PI3K/AKT signaling pathway were the most enriched molecules and pathways. SKN inhibited CRC cell (HT29 and HCT116) proliferation, migration, and invasion and promoted cell apoptosis by targeting IL6 and inhibiting the IL6R/PI3K/AKT signaling pathway.Conclusions: SKN promotes apoptosis and suppresses CRC cell (HT29 and HCT116) activities through the PI3K-Akt signaling pathway. This research not only provides a theoretical and experimental basis for more in-depth studies but also offers an efficient method for the rational utilization of a series of traditional Chinese medicines as anti-CRC drugs.

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“…Candidate potential ingredients and herbals of HFD were retrieved and screened from TCMSP retrieved and TCMID database. All the ingredients and their quantitative targets were visualized analysis using Cytoscape 3.7.1 software [11].…”

Section: Establishment Of Ingredients and Chinese Herbal Medicines Networkmentioning

confidence: 99%

Potential Molecular Target Prediction and Docking Verification of Hua‐Feng‐Dan in Stroke Based on Network Pharmacology

Yang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Objective. Hua-Feng-Dan (HFD) is a Chinese medicine for stroke. This study is to predict and verify potential molecular targets and pathways of HFD against stroke using network pharmacology. Methods. The TCMSP database and TCMID were used to search for the active ingredients of HFD, and GeneCards and DrugBank databases were used to search for stroke-related target genes to construct the “component-target-disease” by Cytoscape 3.7.1, which was further filtered by MCODE to build a core network. The STRING database was used to obtain interrelationships by topology and to construct a protein-protein interaction network. GO and KEGG were carried out through DAVID Bioinformatics. Autodock 4.2 was used for molecular docking. BaseSpace was used to correlate target genes with the GEO database. Results. Based on OB ≥ 30% and DL ≥ 0.18, 42 active ingredients were extracted from HFD, and 107 associated targets were obtained. PPI network and Cytoscape analysis identified 22 key targets. GO analysis suggested 51 cellular biological processes, and KEGG suggested that 60 pathways were related to the antistroke mechanism of HFD, with p53, PI3K-Akt, and apoptosis signaling pathways being most important for HFD effects. Molecular docking verified interactions between the core target (CASP8, CASP9, MDM2, CYCS, RELA, and CCND1) and the active ingredients (beta-sitosterol, luteolin, baicalein, and wogonin). The identified gene targets were highly correlated with the GEO biosets, and the stroke-protection effects of Xuesaitong in the database were verified by identified targets. Conclusion. HFD could regulate the symptoms of stroke through signaling pathways with core targets. This work provided a bioinformatic method to clarify the antistroke mechanism of HFD, and the identified core targets could be valuable to evaluate the antistroke effects of traditional Chinese medicines.

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Exploring Mechanism of Key Chinese Herbal Medicine on Breast Cancer by Data Mining and Network Pharmacology Methods

Cited by 20 publications

References 47 publications

Effects of Chinese Herbal Formula on Immune Function and Nutritional Status of Breast Cancer Patients

Effects of Chinese Herbal Formula on Immune Function and Nutritional Status of Breast Cancer Patients

Network Pharmacology-Based Prediction and Verification of the Mechanism of Shikonin in the Treatment of Colorectal Cancer

Potential Molecular Target Prediction and Docking Verification of Hua‐Feng‐Dan in Stroke Based on Network Pharmacology

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