Exploring linker's sequence diversity to fuse carotene cyclase and hydroxylase for zeaxanthin biosynthesis

Bouin, Aurélie; Zhang, Congqiang; Lindley, Nic D.; Truan, Gilles; Lautier, Thomas

doi:10.1016/j.mec.2023.e00222

Cited by 1 publication

(1 citation statement)

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“…Kavoosi et al have proved that the expression level, proteolytic stability, and thermodynamic stability of the fusion protein expressed in recombinant E. coli can be decided by the chemical properties and the length of the linker (Kavoosi 2007 ). Bouin et al also showed that enzyme orientation and linker length are crucial for the performance of fusion enzymes (Bouin et al 2023 ). The design and selection of valuable linkers should not be based solely on proteolytic stability.…”

Section: Design and Selection Of Linker Between The Fusion Proteinmentioning

confidence: 99%

Research progress of multi-enzyme complexes based on the design of scaffold protein

Wang,

Jiang,

Liu

et al. 2023

Bioresour. Bioprocess.

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Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to increase the production of enzyme cascades, thereby achieving effective biosynthetic pathways. This paper reviews the recent progress in the design strategy and application of multi-enzyme complexes. First, the metabolic channels in the multi-enzyme complex have been introduced, and the construction strategies of the multi-enzyme complex emerging in recent years have been summarized. Then, the discovered enzyme cascades related to scaffold proteins are discussed, emphasizing on the influence of the linker on the fusion enzyme (fusion protein) and its possible mechanism. This review is expected to provide a more theoretical basis for the modification of multi-enzyme complexes and broaden their applications in synthetic biology.

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Section: Design and Selection Of Linker Between The Fusion Proteinmentioning

confidence: 99%

Research progress of multi-enzyme complexes based on the design of scaffold protein

Wang,

Jiang,

Liu

et al. 2023

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

Exploring linker's sequence diversity to fuse carotene cyclase and hydroxylase for zeaxanthin biosynthesis

Cited by 1 publication

References 50 publications

Research progress of multi-enzyme complexes based on the design of scaffold protein

Research progress of multi-enzyme complexes based on the design of scaffold protein

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