Exploring Human Milk Dynamics: Interindividual Variation in Milk Proteome, Peptidome, and Metabolome

Dekker, Pieter; Boeren, Sjef; Goudoever, Johannes B. van; Vervoort, Jacques; Hettinga, Kasper

doi:10.1021/acs.jproteome.1c00879

Cited by 10 publications

(10 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein concentration was determined with the Pierce bicinchonic acid (BCA) assay (Thermo Scientific, Waltham, MA), and samples were diluted in 100 mM Tris to a concentration of 1 µ g/ µ L protein. The diluted skimmed milk samples were prepared with filter-aided sample preparation (FASP) for protein analysis in randomized order as previously described ( 30 ).…”

Section: Methodsmentioning

confidence: 99%

The human milk proteome and allergy of mother and child: Exploring associations with protein abundances and protein network connectivity

Dekker

Azad²,

Boeren³

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

BackgroundThe human milk proteome comprises a vast number of proteins with immunomodulatory functions, but it is not clear how this relates to allergy of the mother or allergy development in the breastfed infant. This study aimed to explore the relation between the human milk proteome and allergy of both mother and child.MethodsProteins were analyzed in milk samples from a subset of 300 mother-child dyads from the Canadian CHILD Cohort Study, selected based on maternal and child allergy phenotypes. For this selection, the definition of “allergy” included food allergy, eczema, allergic rhinitis, and asthma. Proteins were analyzed with non-targeted shotgun proteomics using filter-aided sample preparation (FASP) and nanoLC-Orbitrap-MS/MS. Protein abundances, based on label-free quantification, were compared using multiple statistical approaches, including univariate, multivariate, and network analyses.ResultsUsing univariate analysis, we observed a trend that milk for infants who develop an allergy by 3 years of age contains higher abundances of immunoglobulin chains, irrespective of the allergy status of the mother. This observation suggests a difference in the milk’s immunological potential, which might be related to the development of the infant’s immune system. Furthermore, network analysis showed overall increased connectivity of proteins in the milk of allergic mothers and milk for infants who ultimately develop an allergy. This difference in connectivity was especially noted for proteins involved in the protein translation machinery and may be due to the physiological status of the mother, which is reflected in the interconnectedness of proteins in her milk. In addition, it was shown that network analysis complements the other methods for data analysis by revealing complex associations between the milk proteome and mother-child allergy status.ConclusionTogether, these findings give new insights into how the human milk proteome, through differences in the abundance of individual proteins and protein-protein associations, relates to the allergy status of mother and child. In addition, these results inspire new research directions into the complex interplay of the mother-milk-infant triad and allergy.

show abstract

Section: Methodsmentioning

confidence: 99%

The human milk proteome and allergy of mother and child: Exploring associations with protein abundances and protein network connectivity

Dekker

Azad²,

Boeren³

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…As mentioned previously, all proteins exhibit higher inter-individual variabilities than the variation of the same individual on different days. This trend has also been reported in other biofluids. − Lipocalin-1 and lysozyme C are two of the most abundant proteins in tears, and both exhibited relatively low inter-day and inter-individual variabilities from this analysis. Serum albumin is also a highly abundant protein but interestingly showed a much higher variation.…”

Section: Resultsmentioning

confidence: 99%

Targeted Workflow Investigating Variations in the Tear Proteome by Liquid Chromatography Tandem Mass Spectrometry

Lépine,

Zambito,

Sleno

2023

ACS Omega

View full text Add to dashboard Cite

Proteins in tears have an important role in eye health and have been shown as a promising source of disease biomarkers. The goal of this study was to develop a robust, sensitive, and targeted method for profiling tear proteins to examine the variability within a group of healthy volunteers over three days. Inter-individual and inter-day variabilities were examined to contribute to understanding the normal variations in the tear proteome, as well as to establish which proteins may be better candidates as eventual biomarkers of specific diseases. Tear samples collected on Schirmer strips were subjected to bottom-up proteomics, and resulting peptides were analyzed using an optimized targeted method measuring 226 proteins by liquid chromatography-scheduled multiple reaction monitoring. This method was developed using an in-house database of identified proteins from tears compiled from high-resolution data-dependent liquid chromatography tandem mass spectrometry data. The measurement of unique peptide signals can help better understand the dynamics of each of these proteins in tears. Some interesting trends were seen in specific pathways or protein classes, including higher variabilities for those involved in glycolysis, glutathione metabolism, and cytoskeleton proteins and lower variation for those involving the degradation of the extracellular matrix. The overall aim of this study was to contribute to the field of tear proteomics with the development of a novel and targeted method that is highly amenable to the clinical laboratory using high flow LC and commonly used triple quadrupole mass spectrometry while ensuring that protein quantitation was reported based on unique peptides for each protein and robust peak areas with data normalization. These results report on variabilities on over 200 proteins that are robustly detected in tear samples from healthy volunteers with a simple sample preparation procedure.

show abstract

“…In our studies, we observed a reduction in AOPP in colostrum when the dietary variables of lactating women were not considered. The artificial substances in soft drinks, constituting a significant portion of their composition, promote the generation of free radicals, which have known adverse effects extensively studied, including their association with cardiovascular diseases, Type 2 diabetes, and autism, among other conditions (Dekker et al, 2022; Lu et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

“…Their degradation can lead to changes in their function, thereby altering the formation of the intestinal microbiome. The consequences of the degradation of these nutrients can be unpredictable (Dekker et al, 2022; Dingess et al, 2021; Lawrence, 2022).…”

Section: Introductionmentioning

confidence: 99%

Influence of Maternal Dietary Pattern on the Pro-Oxidant and Antioxidant Status of Mother’s Own Milk

Silva,

Vasconcelos,

Pessoa

et al. 2024

J Hum Lact

View full text Add to dashboard Cite

Background: Human milk is essential for a child’s best development. However, what a mother eats while breastfeeding can directly influence the composition of mother’s milk. Research Aim: This study aimed to assess the antioxidant-oxidant profile of human milk and establish a connection between this profile and the dietary habits of the mothers. Methods: A cross-sectional study was conducted at the Hospital Infantil e Maternidade Alzir Bernardino Alves (HIMABA), located in the municipality of Vila Velha-ES, Brazil. The sample included 98 participants. All volunteers completed a structured interview and a Food Frequency Questionnaire. Approximately 5–10 ml of colostrum, transitional milk, and mature milk were manually collected. The antioxidant activity of human milk was assessed using the colorimetric method for free radical scavenging with 2,2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid. Oxidative stress was determined by measuring lipid peroxidation through malondialdehyde concentration, evaluating advanced oxidation protein products, and assessing total protein content using the Bradford method. Results: The antioxidant profile of colostrum was higher than that observed in later phases of milk, whereas pro-oxidants increased in later phases. Maternal dietary patterns influenced the pro-oxidant status of human milk. Participants with a higher daily intake of milk, dairy products, vegetable oils, olive oils, and legumes exhibited lower levels of lipid peroxidation in colostrum, transition milk, and mature milk, respectively. Conclusions: Our study highlights the vital role of a balanced maternal diet in shaping the pro-oxidant status of human milk, with implications for infant health.

show abstract

Exploring Human Milk Dynamics: Interindividual Variation in Milk Proteome, Peptidome, and Metabolome

Cited by 10 publications

References 74 publications

The human milk proteome and allergy of mother and child: Exploring associations with protein abundances and protein network connectivity

The human milk proteome and allergy of mother and child: Exploring associations with protein abundances and protein network connectivity

Targeted Workflow Investigating Variations in the Tear Proteome by Liquid Chromatography Tandem Mass Spectrometry

Influence of Maternal Dietary Pattern on the Pro-Oxidant and Antioxidant Status of Mother’s Own Milk

Contact Info

Product

Resources

About