Exploring factors affecting anther culture in rice (Oryza sativa L.)

doi:10.7324/jabb.2019.70216

Cited by 24 publications

(7 citation statements)

References 45 publications

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“…These results demonstrated the efficiency of the regeneration medium used. Effect of genotype on plant regeneration from callus cultures have been reported previously (Mon et al, 2020;Sharma et al, 2018;Tripathy et al, 2019). Bagheri andJelodar (2008), andEl-Hennawy et al (2011) reported that plant regeneration from callus was found to be highly heritable.…”

Section: Effect Of Different Drought Stress Treatments On the Anther ...mentioning

confidence: 59%

Combining ability of anther culture response in some rice crosses under drought stress conditions

El-Hennawy,

El-Beially,

El-Refaee

et al. 2023

Archives of Agriculture Sciences Journal

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Anther culture response of ten rice parental genotypes and their 24 F1 crosses was studied to estimate combining ability effects for callus induction and plant regeneration under drought stress conditions. The analysis of variance showed that drought stress levels mean squares were found to be highly significant for all in vitro studied traits. The percentage of anthers that developed calli ranged from 12.92% for the IRRI 148 to 45.00% for the cross L1 × T3 among the genotypes across the four different stress levels of PEG. The control (PEG-free medium) gave the highest mean value of callus induction (41.33%). On the other hand, the medium containing 30 g/l of PEG gave the lowest one (14.28%) across all genotypes. Results showed that the three crosses (L1 × T2, L1 × T3 and L3 × T1) gave the highest mean values of plant regeneration (9.50%, 8.00 and 7.92% respectively). The percentage of plant regeneration from the control medium was 7.12%, while the medium containing 30 g/l of PEG gave the lowest one across all genotypes (3.76%). The ratio of GCA/SCA was found less than unity for all in vitro studied traits under the four stress levels, revealing the predominant role of dominance gene action in the inheritance of these traits. Results showed that the IRGC (T3) followed by Giza 177 (L1) were the best combiners for callus induction under varying concentrations of PEG due to their highly significant positive GCA values. For plant regeneration, one parental genotype Giza 177 (L1) was the best combiner for plant regeneration under varying concentrations of PEG due to their highly significant positive GCA values. The two crosses; L3 × T1 and L1 × T2 recorded the highest SCA effects for plant regeneration and green plant regeneration under the four stress levels.

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Section: Effect Of Different Drought Stress Treatments On the Anther ...mentioning

confidence: 59%

Combining ability of anther culture response in some rice crosses under drought stress conditions

El-Hennawy,

El-Beially,

El-Refaee

et al. 2023

Archives of Agriculture Sciences Journal

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“…Albinism or chlorophyll-deficient regeneration is one of the most important challenges in anther cultures of many cereal crops like barley (Makowska and Oleszczuk 2014), triticale (Krzewska et al 2015), rice (Mishra and Rao 2016), and wheat (Weigt et al 2019). In rice, the frequency of albino plant varies from 5% to 100%, and it is affected by several factors such as the length of cold pre-treatment exposure, genotype, culture medium, and gelling agent (Mishra and Rao 2016;Tripathy et al 2019). The study showed a high frequency of albino plant ranging from 67% in 200 Gy treatment to 100% in 0 Gy and 300 Gy treatments.…”

Section: Development Of Dhs Derived From M1 Plantsmentioning

confidence: 99%

Genetic variation of doubled haploids derived from anther culture of M1 red rice plants

et al. 2021

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Abstract. Nurmansyah, Setyadi AH, Fatumi NC, Fatmawati Y, Wulandari RA, Purwantoro A. 2021. Genetic variation of doubled haploids derived from anther culture of M1 red rice plants. Biodiversitas 22: 4923-4929. The doubled haploid (DH) technology when integrated with induced mutation could accelerate development of local rice cultivars with several desirable traits. Anthers from the first generation of mutant (M1) plants were utilized for DH production. However, the effectiveness of inducing and fixing mutation requires detailed evaluation with the help of molecular techniques for its accuracy and reliability than that of morphological or biochemical assessments. The objective of this research was to develop and detect genetic variation of DH plants derived from anther culture of M1 plants. Seeds of local red rice cultivar, Cempo Abang, were treated with four gamma-ray doses (0, 100, 200, 300 Gy) and planted to produce the M1 plants. Panicles at the booting stage of the M1 plants were collected and used as donor anthers. The present study suggested that gamma irradiation treatments indirectly could increase callus formation. However, it also reduced the number of callus producing plantlets due to higher frequency of brown calli. Eleven spontaneous DH plants obtained in the study along with parental plants were assessed to determine the occurrence of genetic variation using six RAPD primers. The RAPD primers generated 51 bands, of which 34 alleles or 66.7% were polymorphic with an average of 5.6 polymorphic alleles per primer. The genetic similarity among parent and 11 DH lines based on Jaccard’s similarity index ranged from 0.622 to 0.902. The DNA polymorphism among the DH plants demonstrated the effect of gamma irradiation to create genetic variation. Therefore, this method could be used as an alternative for rice breeding programs especially to develop preferred traits in the local rice cultivars.

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“…Na geração de plantas haploides, uma das técnicas utilizadas é o cultivo de anteras (TRIPATHY et al, 2019) para a indução de calos e posterior regeneração dos indivíduos haploides. Os calos têm por base uma massa de proliferação celular, que é induzida em um explante inicial por meio da adição de reguladores vegetais, como auxinas, giberelinas e citocininas, que promovem uma desdiferenciação dos tecidos vegetais (MOSTAFA et al, 2020).…”

Section: Introductionunclassified

Potencial regenerativo de calos induzidos em anteras de Citrus medica L. / Regenerative potential of calluses induced in anthers of Citrus medica L.

Santos¹,

Souza²,

Filho³

et al. 2021

BJDV

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Avanços nos programas de melhoramento de citros podem ser alcançados com a obtenção de indivíduos haploides por meio do cultivo de anteras. As cidras, genótipo utilizado para esse estudo, são reconhecidas por seu valor terapêutico na medicina tradicional. Assim, o objetivo do presente trabalho foi de ajustar um protocolo para a regeneração de plantas de Citrus medica L., a partir de calos originados de anteras. A espécie Citrus medica L. foi estudada visando a regeneração de plantas a partir de calos induzidos por meio de anteras. Foram montados experimentos envolvendo calos

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Exploring factors affecting anther culture in rice (Oryza sativa L.)

Cited by 24 publications

References 45 publications

Combining ability of anther culture response in some rice crosses under drought stress conditions

Combining ability of anther culture response in some rice crosses under drought stress conditions

Genetic variation of doubled haploids derived from anther culture of M1 red rice plants

Potencial regenerativo de calos induzidos em anteras de Citrus medica L. / Regenerative potential of calluses induced in anthers of Citrus medica L.

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