Exploring epistatic relationships of NO biosynthesis pathway genes in susceptibility to CHD

Abstract

Aim: To assess the epistatic relationships of nitric oxide (NO) biosynthesis pathway genes in susceptibility to coronary heart disease (CHD). Methods: A total of 2142 subjects enrolled in two case-control studies was genotyped for 7 single-nucleotide polymorphisms (SNP) within NO biosynthesis pathway genes using TaqMan assays. The association analyses were performed at both SNP and haplotype levels. Two-way SNP-SNP interactions and high-order interactions were tested using multiple unconditional logistic regression analyses and generalized multifactor dimensionality reduction (GMDR) analyses, respectively. Results: Two alleles (rs1049255*C and rs841*A) were identified that were significantly associated with increased risk of CHD after adjusting for all confounders (OR=1.21, 95% CI: 1.06−1.39, combined P=0.001, P corr =0.007 and OR=1.30, 95% CI 1.12−1.50, combined P<0.001, P corr <0.001, respectively). Significant two-way SNP-SNP interactions were found between SNP rs2297518 and these two significant polymorphisms, affecting the risk of CHD (P<0.001 for both). No significant high-order interactions were identified. Conclusion:The results suggested that two-way SNP-SNP interactions of polymorphisms within NO biosynthesis pathway genes contribute to CHD risk.

Genetic differentiation induced by spaceflight treatment of Cistanche deserticola and identification of inter-simple sequence repeat markers associated with its medicinal constituent contents

“…doi:10.1016/j.asr.2010.10.010 the inability of kidney in expelling extra fluid in the body, causing fluid retention, and reform reproductive system, including impotence, infertility, general weakness with aching of the loins and knees and constipation. The Herba Cistanches contains several bioactive compounds, among which acteoside and echinacoside are used to evaluate the therapeutic quality of C. deserticola (Tu et al, 1997).…”

GTP Cyclohydrolase I Gene Polymorphisms Are Associated with Endothelial Dysfunction and Oxidative Stress in Patients with Type 2 Diabetes Mellitus

“…Subsequently, Doehring et al [21] showed that this 3′-UTR polymorphism is in complete LD with the pain-modulating haplotype. Polymorphisms rs1049255 and rs841 were independently associated with coronary artery disease [28] . According to this study, epistatic interactions with other polymorphisms of the NO biosynthesis pathway contribute additionally to the risk of coronary artery disease.…”

The rs9932581 and rs1049255 Polymorphisms in CYBA is not Associated with Preeclampsia in Chinese Han Women

“…So far, the G allele of rs9932581 was regarded as a genetic marker related to hypertension in the Spanish people [18]. And some studies have been indicated that rs1049255 polymorphism affected the risk of ischemic stroke and coronary heart disease [20,33]. In addition, these SNPs were associated with diverse cardiovascular diseases, like coronary artery disease, myocardial infarction and diabetic [16].…”

“…Moreno et al found a higher CYBA expression in the -930G allelic promoter than -930A allelic promoterin hypertension by a functional analysis [18]. As another functional variant, A640G polymorphism(rs1049255) situated at 3'-untranslated region ( 3′-UTR) in CYBA [16], which was associated with an increased risk of coronary heart disease(CHD) in Chinese Han population [20] and was potential usefulness to the analysis of Chronic granulomatous disease (CGD) families [21].…”