Exploring differentially expressed key genes related to development of follicle by RNA-seq in Peking ducks (<i>Anas Platyrhynchos</i>)

Ren, Jinjun; Sun, Changsen; Chen, Li; Hu, Jianhong; Huang, Xuetao; Liu, Xiaolin; Liu, Lizhi

doi:10.1101/483818

Cited by 5 publications

(6 citation statements)

References 28 publications

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“…The differences between these groups might be due to the number of follicles present in the LP and HP ducks which are closely related to the secretion of important reproductive hormones (LH and FSH) as reported by a study that the higher the number of follicles, the greater the number of egg produced in laying geese ( Yang et al, 2019 ). Moreover, a transcriptome study in Peking ducks revealed that variation in egg-laying might be due to the differences in the development of ovaries and follicles which may be closely associated with the ovarian steroid synthesis pathway ( Ren et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata)

Bello

Guo

et al. 2021

Poultry Science

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In China, the low egg production rate is a major challenge to Muscovy duck farmers. Hypothalamus and ovary play essential role in egg production of birds. However, there are little or no reports from these tissues to identify potential candidate genes responsible for egg production in White Muscovy ducks. A total of 1,537 laying ducks were raised; the egg production traits which include age at first egg (days), number of eggs at 300 d, and number of eggs at 59 wk were recorded. Moreover, 4 lowest ( LP ) and 4 highest producing ( HP ) were selected at 59 wk of age, respectively. To understand the mechanism of egg laying regulation, we sequenced the hypothalamus and ovary transcriptome profiles in LP and HP using RNA-Seq. The results showed that the number of eggs at 300 d and number of eggs at 59 wk in the HP were significantly more ( P < 0.001) than the LP ducks. In total, 106.98G clean bases were generated from 16 libraries with an average of 6.68G clean bases for each library. Further analysis showed 569 and 2,259 differentially expressed genes ( DEGs ) were identified in the hypothalamus and ovary between LP and HP, respectively. The KEGG pathway enrichment analysis revealed 114 and 139 pathways in the hypothalamus and ovary, respectively which includes Calcium signaling pathway, ECM-receptor interaction, Focal adhesion, MAPK signaling pathway, Apoptosis and Apelin signaling pathways that are involved in egg production. Based on the GO terms and KEGG pathways results, 10 potential candidate genes (P2RX1, LPAR2, ADORA1, FN1, AKT3, ADCY5, ADCY8, MAP3K8, PXN, and PTTG1) were identified to be responsible for egg production. Further, protein-protein interaction was analyzed to show the relationship between these candidate genes. Therefore, this study provides useful information on transcriptome of hypothalamus and ovary of LP and HP Muscovy ducks.

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Section: Discussionmentioning

confidence: 99%

Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata)

Bello

Guo

et al. 2021

Poultry Science

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“…Sex in birds and mammals is governed by sex chromosomes [1], and sex determination genes on sex chromosomes initiate sex differentiation and regulate the differentiation of gonads into ovaries or testes [2]. A host of critical gonadal differentiation genes have been detected [3,4]. However, screening for novel regulators of male and female embryos is highly necessary to enhance our understanding of gonadal differentiation.…”

Section: Introductionmentioning

confidence: 99%

“…In domestic ducks, several candidate genes involved in the development of plumulaceous feather and flight feather structures were obtained by RNA-Seq [21]. Research in Peking ducks has revealed that a key regulator of duck follicle development in the laying period [3]. In addition, RNA-Seq is also widely used to explore the mechanism of sex differentiation.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of genes related to gonad differentiation and development in Muscovy ducks

Bai

Chen

et al. 2020

BMC Genomics

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Background Sex-related genes play a crucial role in gonadal differentiation into testes or ovaries. However, the genetic control of gonadal differentiation in Muscovy ducks remains unknown. Therefore, the objective of our study was to screen new candidate genes associated with ovarian and testicular development. Results In this study, 24 males before gonadal differentiation (MB), 24 females before gonadal differentiation (FB), 24 males after gonadal differentiation (MA) and 24 females after gonadal differentiation (FA) were selected from Putian Muscovy ducks, forming 4 groups. RNA-Seq revealed 101.76 Gb of clean reads and 2800 differentially expressed genes (DEGs), including 46 in MB vs FB, 609 in MA vs FA, 1027 in FA vs FB, and 1118 in MA vs MB. A total of 146 signalling pathways were enriched by KEGG analysis, among which 20, 108, 108 and 116 signalling pathways were obtained in MB vs FB, MA vs MB, MA vs FA and FA vs FB, respectively. In further GO and KEGG analyses, a total of 21 candidate genes related to gonad differentiation and development in Muscovy ducks were screened. Among these, 9 genes were involved in the differentiation and development of the testes, and 12 genes were involved in the differentiation and development of the ovaries. In addition, RNA-Seq data revealed 2744 novel genes. Conclusions RNA-Seq data revealed 21 genes related to gonadal differentiation and development in Muscovy ducks. We further identified 12 genes, namely, WNT5B, HTRA3, RSPO3, BMP3, HNRNPK, NIPBL, CREB3L4, DKK3, UBE2R2, UBPL3KCMF1, ANXA2, and OSR1, involved in the differentiation and development of ovaries. Moreover, 9 genes, namely, TTN, ATP5A1, DMRT1, DMRT3, AMH, MAP3K1, PIK3R1, AGT and ADAMTSL1, were related to the differentiation and development of testes. Moreover, after gonadal differentiation, DMRT3, AMH, PIK3R1, ADAMTSL1, AGT and TTN were specifically highly expressed in males. WNT5B, ANXA2 and OSR1 were specifically highly expressed in females. These results provide valuable information for studies on the sex control of Muscovy ducks and reveal novel candidate genes for the differentiation and development of testes and ovaries.

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“…While there has been a push toward applying transcriptome methods in ornithological studies over the recent years (Jax, Wink, & Kraus, 2018), little is known about the age‐specific gene expression in birds. Age‐specific gene expression in follicles of Peking ducks ( Anas platyrhynchos ) indicated no clear pattern regarding expression levels in relation to age (Ren et al., 2019), while in great tits ( Parus major ), age classes were not associated with differential gene expression levels in blood and liver (Watson, Videvall, Andersson, & Isaksson, 2017). Conversely, in the present study, we found that more than 90% of the differentially expressed genes (including also transcripts that were not related to cancer) were downregulated in older birds.…”

Section: Discussionmentioning

confidence: 99%

Age‐dependent expression of cancer‐related genes in a long‐lived seabird

Meitern

Fort

Giraudeau

et al. 2020

Evolutionary Applications

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Studies of model animals like mice and rats have led to great advances in our understanding of the process of tumorigenesis, but this line of study has less to offer for understanding the mechanisms of cancer resistance. Increasing the diversity of nonmodel species from the perspective of molecular mechanisms of natural cancer resistance can lead to new insights into the evolution of protective mechanisms against neoplastic processes and to a wider understanding of natural cancer defense mechanisms. Such knowledge could then eventually be harnessed for the development of human cancer therapies. We suggest here that seabirds are promising, albeit currently completely ignored candidates for studying cancer defense mechanisms, as they have a longer maximum life span than expected from their body size and rates of energy metabolism and may have thus evolved mechanisms to limit neoplasia progression, especially at older ages. We here apply a novel, intraspecific approach of comparing old and young seabirds for improving our understanding of aging and neoplastic processes in natural settings. We used the long‐lived common gulls (Larus canus) for studying the age‐related pattern of expression of cancer‐related genes, based on transcriptome analysis and databases of orthologues of human cancer genes. The analysis of differently expressed cancer‐related genes between young and old gulls indicated that similarly to humans, age is potentially affecting cancer risk in this species. Out of eleven differentially expressed cancer‐related genes between the groups, three were likely artifactually linked to cancer. The remaining eight were downregulated in old gulls compared to young ones. The downregulation of five of them could be interpreted as a mechanism suppressing neoplasia risk and three as increasing the risk. Based on these results, we suggest that old gulls differ from young ones both from the aspect of cancer susceptibility and tumor suppression at the genetic level.

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Exploring differentially expressed key genes related to development of follicle by RNA-seq in Peking ducks (Anas Platyrhynchos)

Cited by 5 publications

References 28 publications

Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata)

Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata)

Transcriptome analysis of genes related to gonad differentiation and development in Muscovy ducks

Age‐dependent expression of cancer‐related genes in a long‐lived seabird

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