Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells

Chaves, Natália Lemos; Estrela‐Lopis, Irina; Böttner, Julia; Lopes, C.A.P.; Guido, Bruna C.; Souza, Aparecido Ribeiro de; Báo, Sônia Nair

doi:10.2147/ijn.s141582

Cited by 58 publications

(38 citation statements)

References 45 publications

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“…Cancer cells possess a higher metabolic activity to normal cells and their surface tends to overexpress various receptors which increase the available binding sites and therefore in turn promote a higher uptake efficiency of particles and drugs [81][82][83].…”

Section: Morphology Characterizationmentioning

confidence: 99%

Magnetic Alginate / Chitosan Nanoparticles for Targeted Delivery of Curcumin into Human Breast Cancer Cells

Song

Gregory

et al. 2018

Preprint

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Curcumin is a promising anti-cancer drug but its applications in cancer therapy are limited due to its poor solubility, short half-life and low bioavailability. In this study, curcumin loaded magnetic alginate / chitosan nanoparticles were fabricated to improve the bioavailability, uptake efficiency and cytotoxicity of curcumin to MDA-MB-231 breast cancer cells. Alginate and chitosan were deposited on Fe3O4 magnetic nanoparticles based on their electrostatic properties. The sizes of the nanoparticles (120-200 nm) were within the optimum range for drug delivery. Sustained curcumin release was obtained use the nanoparticles with the ability to control the curcumin release rate by altering the number of chitosan and alginate layers. Confocal fluorescence microscopy results showed that targeted delivery of curcumin with the aid of magnetic field were achieved. The FACS assay indicated that MDA-MB-231 cells treated with curcumin loaded nanoparticles had a 3-6 folds uptake efficiency to those treated with free curcumin. MTT assay indicated that the curcumin loaded nanoparticles exhibited significantly higher cytotoxicity toward MDA-MB-231 cells than toward HDF cells. The sustained release profiles, enhanced uptake efficiency and cytotoxicity to cancer cells as well as the targeting potential make MACPs a promising candidate for cancer therapy.

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Section: Morphology Characterizationmentioning

confidence: 99%

Magnetic Alginate / Chitosan Nanoparticles for Targeted Delivery of Curcumin into Human Breast Cancer Cells

Song

Gregory

et al. 2018

Preprint

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“…However, the antioxidant role of FFA may be carried out when still interacting or trapped inside the MCM-41, within the 48 h chosen as the timepoint for the in vitro biocompatibility assay, as shown below. The same behavior is possible in vivo, once the timepoint is long enough to allow for the endocytosis of the MSN by the cells, through mechanisms yet to be elucidated, where the FFA is likely to scavenge the free radicals, in case the cells are exposed to harmful stimuli that may tip the oxidation/reduction balance, leading to apoptosis of normal cells [42]. Moreover, a similar trend in drug release was observed for another non-steroidal drug analog to FFA—mefenamic acid—showing a first-order release profile [43].…”

Section: Resultsmentioning

confidence: 99%

Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles

et al. 2018

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Recently, flufenamic acid (FFA) was discovered among fenamates as a free radical scavenger and gap junction blocker; however, its effects have only been studied in cancer cells. Normal cells in the surroundings of a tumor also respond to radiation, although they are not hit by it directly. This phenomenon is known as the bystander effect, where response molecules pass from tumor cells to normal ones, through communication channels called gap junctions. The use of the enhanced permeability and retention effect, through which drug-loaded nanoparticles smaller than 200 nm may accumulate around a tumor, can prevent the local side effect upon controlled release of the drug. The present work, aimed at functionalizing MCM-41 (Mobil Composition of Matter No. 41) silica nanoparticles with FFA and determining its biocompatibility with human fibroblasts MRC-5 (Medical Research Council cell strain 5). MCM-41, was synthesized and characterized structurally and chemically, with multiple techniques. The biocompatibility assay was performed by Live/Dead technique, with calcein and propidium–iodide. MRC-5 cells were treated with FFA-grafted MCM-41 for 48 h, and 98% of cells remained viable, without signs of necrosis or morphological changes. The results show the feasibility of MCM-41 functionalization with FFA, and its potential protection of normal cells, in comparison to the role of FFA in cancerous ones.

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“…By coating this particles with a polymer and further label them with a fluorescent agent, they can be used both for the improvement of MRI imaging and for tracking cells by fluorescence microscopy. In a recent study, Chaves et al (2017) showed that maghemite nanoparticles were more intensively internalized by breast cancer cells as compared to normal cells (Chaves et al, 2017). In addition, the invasive breast metastatic cells -MDA-MB-231 presented a higher uptake of the nanoparticles as MCF7 cells (non-metastatic cells).…”

Section: Farcas Et Almentioning

confidence: 99%

“…However, this value is also cell-type dependent (Hoshyar et al, 2016;Behzadi et al, 2017). Other studies showed that 500 nm particles can be internalized by cells via clathrin-mediated endocytosis (Chaves et al, 2017). Hinds et al (2003) showed that MIOPs can be effectively endocytosed by various cells and can thus contribute to the improvement of MRI (magnetic resonance imaging) signal (Hinds et al, 2003).…”

Section: Farcas Et Almentioning

confidence: 99%

Controlled Synthesis and Characterization of Micrometric Single Crystalline Magnetite With Superparamagnetic Behavior and Cytocompatibility/Cytotoxicity Assessments

et al. 2020

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A new class of magnetite (Fe 3 O 4) particles, coined as "Single Crystalline Micrometric Iron Oxide Particles" (SCMIOPs), were obtained by hydrothermal synthesis. Both the single Fe 3 O 4 phase content and the particle sizes range, from 1 µm to 30 µm, can be controlled by synthesis. The notable finding states that these particles exhibit vanishing remanent magnetization (sr=0.28 emu/g) and coercive force (Hc=1.5 Oe), which indicate a superparamagnetic-like behavior (unexpected at micrometric particles size), and remarkably high saturation magnetization (ss=95.5 emu/g), what ensures strong magnetic response, and the lack of agglomeration after the magnetic field removal. These qualities make such particles candidates for biomedical applications, to be used instead of magnetic nanoparticles which inevitably involve some drawbacks like aglommeration and insufficient magnetic response. In this sense, cytocompatibility/ cytotoxicity tests were performed on human cells, and the results have clearly indicated that SCMIOPs are cytocompatible for healthy cell lines HaCaT (human keratinocytes) and HEMa (primary epidermal melanocytes) and cytotoxic for neoplastic cell lines A375 (human melanoma) and B164A5 (murine melanoma) in a dose-dependent manner.

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Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells

Cited by 58 publications

References 45 publications

Magnetic Alginate / Chitosan Nanoparticles for Targeted Delivery of Curcumin into Human Breast Cancer Cells

Magnetic Alginate / Chitosan Nanoparticles for Targeted Delivery of Curcumin into Human Breast Cancer Cells

Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles

Controlled Synthesis and Characterization of Micrometric Single Crystalline Magnetite With Superparamagnetic Behavior and Cytocompatibility/Cytotoxicity Assessments

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