Exploring cell death mechanisms in spheroid cultures using a novel application of the RIP3-caspase3-assay
C. I. Philippi,
J. Hagens,
K. M. Heuer
et al.
Abstract:This study explores the application of the RIP3-caspase3-assay in heterogeneous spheroid cultures to analyze cell death pathways, emphasizing the nuanced roles of apoptosis and necroptosis. By employing directly conjugated monoclonal antibodies, we provide detailed insights into the complex mechanisms of cell death. Our findings demonstrate the assay’s capability to differentiate between RIP1-independent apoptosis, necroptosis, and RIP1-dependent apoptosis, marking a significant advancement in organoid researc… Show more
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