Exploring bisphenol S removal mechanism with multi-enzymes extracted from waste sludge and reed sediment

Abstract

BPS, as a widespread environmental hormone-like micro-pollutants, is di cult to be degraded in the environment. In this study, the removal of BPS with multi-enzymes extracted from waste sludge and reed sediment were studied at 25℃, 37℃ and 55℃. Results show that BPS could be removed e ciently, which could involve enzymolysis and bio-occulation. The mechanism and pathways of the enzymolysis were identi ed with LC-MS/MS. Polymerization of BPS with enzymolysis further improved the removal by bio-occulation due to the production of BPS oligomers. Furthermore, the interaction mechanism between BPS and multi-enzymes was explored through a series of spectroscopic experiments. Results show that more loose skeletal structure of the multi-enzymes and more hydrophobic microenvironment of the amino acid residues are responsible for the removal of BPS. This research not only provided a method for refractory micropollutants removal but also a way for the utilization of waste sludge and reed sediment.