Exploring bioaugmentation strategies for azo-dye decolorization using a mixed consortium of Pseudomonas luteola and Escherichia coli

Chen, Bor Yann; Chen, Shan Yu; Meng, Lin; Chang, Jo Shu

doi:10.1016/j.procbio.2006.03.004

Cited by 39 publications

(43 citation statements)

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“…Azo dye decolorization by mixed as well as pure cultures, generally, requires organic sources such as glucose, starch, acetate, ethanol, peptone yeast extract, or a combination of complex organic sources and carbohydrates [41,42,48,49,76,84,85,95,109]. As a result of the anaerobic reduction step, a variety of colorless aromatic compounds are synthesized.…”

Section: Cosubstratesmentioning

confidence: 99%

“…Before the advent of such techniques, the key microbial species in wastewater treatment plants were either unknown or sometimes inefficient bacteria were considered important for the various processes. Up until the last decade, very few studies employed molecular tools to monitor the degrader communities in activated sludge systems [142,[151][152][153][154][155], whereas more recently the use of such tools has become increasingly common for monitoring microbial compositions and the activity of dye degraders in water treatment systems [42,147,150,[156][157][158]. Ultimately, these techniques should prove useful to identify the links between microbial community composition, function, and process stability.…”

Section: System Ecology: Features Of Wastewater Treatment Systemsmentioning

confidence: 99%

“…Although conventional activated sludge systems are commonly used to treat azo dye containing wastewater [16, 29, 70-72, 172, 173], these treatment systems are inconsistent for removal of recalcitrant azo dyes and are subject to failure due to poor environmental conditions [32, 42,43,174]. The bioaugmentation of treatment systems commonly involves the use of mixed cultures of microorganisms (Table 4), and similarly can result in varying treatment efficacy depending on the abilities of the individual strains to compete with indigenous populations that are often wellacclimated to the existing environmental conditions [191].…”

Section: Bioaugmentation With Azo Dye Degrading Bacteriamentioning

confidence: 99%

“…Often the effectiveness of individual isolates can be enhanced by co-culture with other highly efficient dye-decolorizing strains [42][43][44]. Here, it is speculated that the combined enzyme systems of the mixed bacterial culture are more effective than the enzymes from the individual isolates, each of which may have different substrate kinetics and efficiency at different dye concentrations.…”

mentioning

confidence: 99%

“…Cooperation within microbial communities also can occur through exchange of growth cofactors and removal of toxic metabolites. Although many microorganisms can degrade azo dyes [7,[40][41][42][45][46][47][48][49][50][51], relatively few microbial species and strains have emerged as candidates for use in bioaugmentation [14, 40,[52][53][54][55]. Before individual isolates can be recommended, comprehensive research is required to understand the role of individual microorganisms and their interactions with other microflora [24,35,56].…”

mentioning

confidence: 99%

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Bioaugmentation of Azo Dyes

Khalid

Arshad

Crowley

2010

The Handbook of Environmental Chemistry

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Biodegradation is a cost-effective method to remove the residues of azo dyes prior to their discharge in wastewater streams from dye product industries. The efficacy of this treatment method is highly dependent on establishing an effective degrader community and maintaining environmental conditions that support the growth and activity of the degrader organisms. Although activated sludge is commonly used as a source of degrader organisms to start the process, bioaugmentation of the wastewater with highly effective strains provides a much more reliable process in which the process manager can use bacterial strains that target particular dye chemicals and metabolites to achieve complete mineralization. The most effective inoculants are able to degrade dyes over a broad concentration range, tolerate a range of environmental conditions of temperature, pH, and salinity, and persist at high population densities in competition with other microorganisms in mixed microbial cultures. The use of growth supplements such as yeast extract can further enhance the biodegradation activity. The ability to achieve complete mineralization of azo dyes depends on the control of the process in which initial decolorization takes place under microaerophilic conditions with low oxygen, followed by elimination of the dye metabolites using an aeration step. In many cases, this may be best achieved by using a mixture of bacterial strains that sequentially carry out the two-step process. Practical development of bacteria for bioaugmentation requires careful screening that is based not only on their efficacy in pure culture, but also on their ability to compete with the indigenous microbial communities in wastewater streams and ability to be produced and delivered as a stable inoculum. In the future, it may be useful to consider bioaugmentation with bacteria that contain mobile genetic elements that carry catabolic pathways, thereby allowing the genes to be introduced into the indigenous microorganisms. The ability to monitor introduced bacteria or catabolic genes will continue to be important for process optimization both in the laboratory and during operation in fullscale treatment systems.

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Section: Cosubstratesmentioning

confidence: 99%

Section: System Ecology: Features Of Wastewater Treatment Systemsmentioning

confidence: 99%

Section: Bioaugmentation With Azo Dye Degrading Bacteriamentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Bioaugmentation of Azo Dyes

Khalid

Arshad

Crowley

2010

The Handbook of Environmental Chemistry

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Bioaugmentation of p‐chloronitrobenzene in bioelectrochemical systems with Pseudomonas fluorescens

Song

Zhou

Wang

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: p-Chloronitrobenzene (p-CNB) is easily accumulated in the environment and subsequently is a threat to humans and the ecosystem. The anaerobic process as an important pathway for p-CNB degradation has a relatively low degradation rate. This study illustrated that the strain Pseudomonas fluorescens was evaluated as the biocatalyst for bioaugmentation to enhance the p-CNB removal in bioelectrochemical system (BES). RESULTS:When the initial p-CNB concentration was 100 mg L −1 , the p-CNB removal efficiency reached 100% at 12 h in BES with P. fluorescens. All the p-CNB removal efficiencies in BES were higher than that in anaerobic degradation and electrocatalysis. Meantime, the highest total organic carbon (TOC) removal efficiency was obtained at 89.8% after augmentation with P. fluorescens. During the bioaugmentation, microbial community analysis showed that the main abundance changes were in Pseudomonas, Romboutsia and Macellibacteroides. The cyclic voltammetry (CV) showed BES with bioaugmentation had the highest activity in reducing p-CNB and hydrogen evolution. Combined with intermediates analysis, bioaugmentation possibly stimulated nitro reduction and hydrodechlorination rate in p-CNB reduction.CONCLUSION: These results demonstrate that BES with P. fluorescens bioaugmentation could serve as a potential treatment process for p-CNB removal. Microbial communitySamples were collected from biofilm on the cathode in BES-I, BES-IP and AEC-I at the end of the experiment using bacterial 16S rDNA sequence analysis on an Illumina platform. The PowerSoil ® J Chem Technol Biotechnol 2020; 95: 274-280

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Mixed consortia in bioprocesses: role of microbial interactions

Ghosh

Chowdhury

Bhattacharya³

2016

Appl Microbiol Biotechnol

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The utilization of mixed consortia or mixed culture has become a current research trend of applied microbiology, bioprocess engineering and biotechnology. The constituent microorganisms of such mixed cultures can jointly perform complex processes efficiently, yielding the desired product at an augmented rate, in comparison to monocultures. It is understandable that the interactions between the microbial partners in these mixed cultures are expected to have a significant impact on the combined performance of the microorganisms and the bioprocess as a whole. Prevalence of positive interactions (commensalism or mutualism) among microbial members of a mixed culture or consortia can significantly enhance the product outcome of the bioprocess, ensuring their industrial application and long-term stability. On the contrary, negative interaction (parasitism, predation or ammensalism) leads to elimination of microbial members from the consortia causing the destruction of community structure as well as disruption of cumulative performance. Therefore, a priori knowledge on the type of interaction between the microorganisms is also essential for the optimization of the performance of the designed consortia. This could only be achieved through the study of inter-microbial interaction prevailing in a mixed culture. In the present article, different bioprocess applications of mixed cultures, currently in practice along with types of positive microbial interactions involved, have been reviewed. Complexity of mixed cultures from the perspective of multiple types of intra-culture relationships has been explained in detail. Overall, the necessity for more in-depth research studies on "microbial interaction" in mixed culture bioprocesses has been stressed in the article.

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Exploring bioaugmentation strategies for azo-dye decolorization using a mixed consortium of Pseudomonas luteola and Escherichia coli

Cited by 39 publications

References 30 publications

Bioaugmentation of Azo Dyes

Bioaugmentation of Azo Dyes

Bioaugmentation of p‐chloronitrobenzene in bioelectrochemical systems with Pseudomonas fluorescens

Mixed consortia in bioprocesses: role of microbial interactions

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