Exploring bioaugmentation strategies for azo dye CI Reactive Violet 5 decolourization using bacterial mixture: dye response surface methodology

Abstract

The use of response surface methodology based on statistical design of experiments is becoming increasingly widespread in several sciences such as analytical and environmental chemistry. In the present study, the decolourization and the degradation efficiency of CI Reactive Violet 5 (CIRV5) was studied using a novel bacterial consortium. CIRV5 (1000 p.p.m.) biodegradation was investigated under shaking condition in mineral salt medium solution (MSM) at a 7.5 pH and a 25°C temperature. The degradation pathways were also predicted, using UV‐visible spectroscopy analysis. Under optimal conditions, the bacterial consortium was able to decolourize the dye completely (>99%) within 8 h. The colour and chemical oxygen demand (COD) removal were 99.29 and 94.93%, respectively. Polymerase chain reaction (PCR) technique was utilized to detect the adhesin genes ‘icaA’ and ‘icaD.’ Our results showed that Staphylococcus aureus had a high decolourization capacity. Phytotoxicity study using Triticum turgidum ssp durum showed the no toxicity of the produced products.

Photocatalytic Degradation of Azo Dye Reactive Violet 5 on Fe-Doped Titania Catalysts under Visible Light Irradiation

“…Finally, Ayed et al [31] explored the feasibility of using a consortium of Staphylococcus species, two of which were isolated from a textile wastewater, to degrade RV5. Under optimal conditions, the dye solution was completely (>99%) decolorized within 8 h. The decolorization was also accompanied by a high COD (Chemical Oxygen Demand) removal (94.93%) Therefore, the results obtained in the present study indicate that the degradation of RV5 by a Fe-doped titania catalyst under visible light irradiation can lead to dye removal efficiencies that are comparable with those reported for UV-photocatalytic or microbial treatments.…”

“…However, its complex aromatic structure ( Figure 1) makes it highly resistant to degradation. A literature survey showed that very few studies have been performed on RV5 degradation and none of them investigated the photocatalytic removal of the dye under visible light [27][28][29][30][31][32]. Accordingly, a first aim of this study was to evaluate whether the use of the selected photocatalyst in the presence of visible light could be a suitable method to degrade RV5.…”

Process optimization for effective bio‐decolourization of methyl orange by Pseudomonas aeruginosa 23N1 using chemometric methodology

“…Moreover, the correlation coefficient (R 2 = 0.999) with a predicted correlation coefficient (Pred R 2 = 0.996) and adjusted correlation coefficient (adj R 2 =0.998) signifies that this model might be used to predict response quite efficiently for a given process condition or vice versa. The higher R 2 value reveals a better representation of experimental data by the developed quadratic regression model . The adequate precision value of 97.72 indicates a sufficient signal to noise ratio.…”

“…The confidence interval around this lambda includes current point value of 1, which matches the model design value. Hence, further transformation is not necessary for the observed response during model fitting . Figure d shows leverage versus run the plot of the quadratic model, which ensures that all numerical values are obtained within the standard limits range of 0–1 of an experimental trial.…”

Role of pH in the Aqueous Phase Reactivity of Zerovalent Iron Nanoparticles with Acid Orange 7, a Model Molecule of Azo Dyes

“…Synthetic dyes are used in many fields, such as textile, plastic, cosmetic, and tannery industries: approximately 800.000 tons of dyes are produced every year all over the world, their global demand being predicted yet to grow in the next years [1][2][3][4].…”