Exploring Antagonistic Metabolites of Established Biocontrol Agent of Marine Origin

Rane, M. R.; Sarode, Prashant; Chaudhari, Bhushan L.; Chincholkar, S. B.

doi:10.1007/s12010-008-8288-y

Cited by 31 publications

(10 citation statements)

References 26 publications

(24 reference statements)

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“…Further purification was performed according to the method of Rane et al . (). HPLC‐MS analysis showed that the pyocyanin component did not contain other metabolites.…”

Section: Methodsmentioning

confidence: 97%

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Anticancer effects of pyocyanin on HepG2 human hepatoma cells

Zhao

Alfred

et al. 2014

Lett Appl Microbiol

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Significance and Impact of the Study: Pyocyanin is a redox-active phenazine toxin. Here, we investigated the ability of pyocyanin to inhibit cancer-related phenotypes in HepG2 human hepatoma cells. Our results indicated that pyocyanin accelerated cellular senescence and apoptosis and induced oxidative stress-associated DNA damage in HepG2 cells. The potential anticancer applications of pyocyanin should be investigated further in clinical studies. AbstractPyocyanin, a major virulence factor produced by Pseudomonas aeruginosa, displays redox activity and damaging effects on mammalian cells. In this study, we investigated the effects of pyocyanin on the proliferation of HepG2 tumour cells. Interestingly, pyocyanin significantly inhibited cell proliferation and triggered the production of large amounts of reactive oxygen species (ROS), thereby upregulating superoxide dismutase (SOD) and catalase (CAT). Additionally, pyocyanin treatment significantly depleted reduced glutathione (GSH) and decreased the GSH/oxidized GSH (GSSG) ratio. These results supported that pyocyanin-induced cytotoxicity in HepG2 cells was mediated by acute ROS production and subsequent oxidative stress. SA-b-Gal, acridine orange (AO)/ethidium bromide (EB) double staining, caspase-3 measurements and comet assay results revealed that cell death induced by pyocyanin involved DNA damage and activation of caspase-3, accelerating cell senescence and apoptosis. Thus, our data provided insights into the mechanisms underlying pyocyanin-induced cytotoxicity and may lead to better treatment strategies for cancer.

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“…Further purification was performed according to the method of Rane et al . (). HPLC‐MS analysis showed that the pyocyanin component did not contain other metabolites.…”

Section: Methodsmentioning

confidence: 97%

“…The fermentation broth was then centrifuged to remove the cell mass, and the supernatant was extracted with an equal volume of chloroform. Further purification was performed according to the method of Rane et al (2008). HPLC-MS analysis showed that the pyocyanin component did not contain other metabolites.…”

Section: Preparation Of Pyocyaninmentioning

confidence: 99%

Anticancer effects of pyocyanin on HepG2 human hepatoma cells

Zhao

Alfred

et al. 2014

Lett Appl Microbiol

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“…It has been reported that a lower concentrations of pyocyanin act as inhibitors (Hassanein et al, 2009). Rane et al (2008) also reported the biocontrol activity of pyocyanin against C. falcatum, F. oxysporum, and Sclerotium rolfsii. In conclusion, although encouraging results were achieved from using phenazines to control G. boninense in vitro, studies must be done to confirm their efficacy in vivo.…”

Section: Discussionmentioning

confidence: 97%

Detection of Phenazines from UPMP3 Strain of Pseudomonas aeruginosa and its Antagonistic Effects against Ganoderma boninense

Parvin¹,

Othman²,

Jaafar³

et al. 2016

IJAB

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To cite this paper: Parvin, W., R. AbstractTo identify the phenazine antibiotics from the selected UPMP3 strain of Pseudomonas aeruginosa and assessment of its antagonistic activity of Ganoderma boninense, three antibiotics phenazine (PHZ), phenazine-l-carboxylic acid (PCA) and pyocyanin (PYO) were extracted from the bacterial fermented broth using benzene and chloroform and detected through High-performance liquid chromatography (HPLC). The effects of three antibiotics on suppressing mycelial growth of G. boninense were assayed. The maximum amount of PHZ, PCA and PYO were recorded as 1.36, 9.62 and 15.48 µg/mL, respectively and showed in vitro antagonistic activity against G. boninense. Phenazine was found to be more effective than PCA and PYO to inhibit the mycelial growth of G. boninense. It was 100% for PHZ at 1.0 mg/mL, while 78.61% for PCA and 91.87% for PYO after 7 days of incubation. Phenazine, PCA and PYO were identified as first time from oil palm rhizospheric UPMP3 strain of P. aeruginosa. The results indicated that the identified PHZ, PCA and PYO compound had good antifungal activity against G. boninense, however the PHZ can be developed as an ideal biofungicide for the control basal stem rot disease of oil palm and helps to overcome the concerns about the residual effects of synthetic fungicides.

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“…In addition, Lahkar et al (2015) revealed the ability of the strain JS29 to inhibit the growth of Alternaria solani, the causal agent of tomato blight, by 73%. Meanwhile, P. aeruginosa ID 4365 and SBC 5 were demonstrated to have good antifungal ability against Sclerotium rolfsii, the causal agent of Southern blight on vegetables and melons (Rane et al, 2008).…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Isolation and Molecular Identification of a Siderophore Producing Bacterium and its Antagonistic Effect against Fusarium oxysporum f. sp. cubense Tropical Race 4

Ch’ng¹,

Yong²,

Othman³

et al. 2022

JTAS

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Bananas are one of the world’s most consumed fruits. Developing countries in the Global South depend on bananas for food security and livelihoods. Still, the banana industry also drives a multinational trade worth billions of US dollars. In addition, banana plants also hold cultural and religious significances in many Asian countries. However, banana production faces several challenges, and one of the major issues is the Fusarium wilt disease caused by the fungus Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4). This disease threatens numerous banana cultivars, including the Cavendish, the most traded banana cultivar. Therefore, the objective of this study was to find effective measures to control the spread of this disease through antagonistic soil bacteria. This study isolated 14 fluorescent, siderophore-producing bacteria with in vitro inhibition rates of 21.73-50.38% against Foc TR4 from the soil surrounding banana plants. Most of the isolates were identified as Pseudomonas spp. via 16S rRNA sequencing, and phylogenetic analysis based on 16s rRNA sequences showed clustering of isolates congruent with the results of similarity searches. Three isolates that exhibited relatively higher antagonistic activity against Fusarium oxysporum f. sp. cubense compared to other isolates were JBAA132 (50.38%), K2B131 (46.28%), and KTP231 (45.38%). Isolate JBAA132 displayed differences in biochemical characteristics compared to its closest match (Pseudomonas aeruginosa type strain DSM50071). It also demonstrated nitrogen-fixing and phosphate solubilising properties common to plant growth promoters. Therefore, isolate JBAA132 may be further explored as a potential biocontrol agent in curbing the spread of Foc TR4.

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Exploring Antagonistic Metabolites of Established Biocontrol Agent of Marine Origin

Cited by 31 publications

References 26 publications

Anticancer effects of pyocyanin on HepG2 human hepatoma cells

Anticancer effects of pyocyanin on HepG2 human hepatoma cells

Detection of Phenazines from UPMP3 Strain of Pseudomonas aeruginosa and its Antagonistic Effects against Ganoderma boninense

Isolation and Molecular Identification of a Siderophore Producing Bacterium and its Antagonistic Effect against Fusarium oxysporum f. sp. cubense Tropical Race 4

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