Explore the possible pathway of improving liver and heart injury in diabetes nephropathy based on bioinformatics analysis

Abstract

Background This study explore the possible pathway of MicroRNA-130a, TXNIP, CD44 and TGF-β1 improving liver and heart injury in diabetes nephropathy based on bioinformatics ananlysis.Methods Screening Key Genes Using Bioinformatics Analysis. The biochemical index and serum levels of MicroRNA-130a, TXNIP, CD44 and TGF-β1 were detected and analyzed by Pearson correlation analysis in 100 DN patients and 50 healthy controls. The rats model were randomly divided into two groups. The expression of MicroRNA-130a, TXNIP, CD44 and TGF-β1 in liver and heart and the morphological changes was detected.Results Screening and Analysis of Differentially Expressed Genes MicroRNA-130a and TXNIP, CD44 and TGFBI Involved in diabetes Nephropathy by Bioinformatics Methods. Compared to healthy controls, serum levels of MicroRNA-130a were decreased, while levels of TXNIP, CD44 and TGF-β1 were elevated in DN patients. Moreover, MicroRNA-130a was negatively correlated with TXNIP, CD44 and TGF-β1. In DN rats, the levels of TXNIP, CD44 and TGF-β1 in the liver and heart tissues were significantly elevated, while MicroRNA-130a levels were significantly decreased, compared to the NC group.Conclusion Upregulate MicroRNA-130a and decrease TXNIP, CD44 and TGF-β1 may participate in liver and heart injury pathway of diabetes nephropathy.