Exploratory in vivo biophysical studies of stratum corneum lipid organization in human face and arm skin

Gorcea, Mihaela; Lane, Majella E.; Moore, David

doi:10.1016/j.ijpharm.2022.121887

Cited by 4 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Absorption by the skin is one of the biggest issues associated with use of peptide treatments, including GHK-Cu, which is not only ascribable to hydrophilicity but also to the highly dense brick-wall structure of the outermost stratum corneum of the skin that blocks most substances from penetrating. [24][25][26][27] Therefore, in vitro permeation experiments using isolated animal skin were used to effectively predict in vivo substance-permeation efficiency. We examined [Bet][Tar]-encapsulated GHK-Cu, with an aqueous solution of GHK-Cu used as the control.…”

Section: Ionic-liquid-based Delivery Carriers Enhance Ghk-cu Transder...mentioning

confidence: 99%

A novel delivery vehicle for copper peptides

Liu

Lu²,

Lü³

et al. 2023

New J. Chem.

View full text Add to dashboard Cite

show abstract

Section: Ionic-liquid-based Delivery Carriers Enhance Ghk-cu Transder...mentioning

confidence: 99%

A novel delivery vehicle for copper peptides

Liu

Lu²,

Lü³

et al. 2023

New J. Chem.

View full text Add to dashboard Cite

show abstract

“…The stratum corneum (SC) is the outermost layer of human skin, with a thickness of 10–20 μm, making it highly resistant to external factors such as UV rays and pathogens and, thus, the most difficult to penetrate . Corneocytes are embedded in a lipid matrix to form a “brick-and-mortar” structure” .…”

Section: Introductionmentioning

confidence: 99%

An Investigation into the Transdermal Behavior of Active Ingredients by Combination of Experiments and Multiscale Simulations

Duan,

Ye,

Zhou

et al. 2024

J. Phys. Chem. B

View full text Add to dashboard Cite

“…These are highly organized into three perpendicular layers, with a rigid head group region, a rigid chain region, and a liquid-like overlapping middle layer [8,10]. When compared to other factors such as thickness of the SC, the quantitative changes in lipid content appear to be the most important in predicting skin permeability [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Novel Skin Emollient Cream on Skin Lipidome and Lipid Organization

Jacques¹,

Dejean²,

Klose³

et al. 2023

Skin Pharmacol Physiol

View full text Add to dashboard Cite

Introduction: The stratum corneum (SC) matrix is composed of free fatty acids, cholesterol and ceramides (CERs), which play a key role in the skin barrier function. Changes in the composition and content of skin lipids will affect the function of the skin barrier. The effect of a glycerol/petrolatum-based emollient (G/P-emollient) cream on the lipid profiles of isolated ex vivo human SC and the SC of a reconstructed human epithelial (RHE) model were measured. Methods: The spatial organization of the cream and the isolated SC intercellular matrix was studied using X-ray diffraction. The inter-bilayer distance in the multi-lamellar lipid structures and lattice type were analyzed using small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS), respectively. Lipidomic analysis using Shotgun Lipidomics was performed on reconstructed human epidermis (RHE) to quantify CER classes and chain lengths. This technology enables the analysis of thousands of lipids in a single biological sample. Results: The crystallized components of the cream are lipids, which were mainly packed in orthorhombic lattices, as well as hexagonal lattices and were similar to the SC structure. The cream penetrated the SC but did not alter the WAXS profile. It increased the amount of higher carbon number ceramides (> 42 carbons) and decreased lower carbon number ceramides (<42 carbons). All chain length acyl-CERs and acyl-CER classes (CER EOS, EOH, EOP, EOdS) were increased as the total CER classes. A decrease of the CER C34 for hydroxylated and non-hydroxylated CERs was also observed. The cream altered the [S] and [P] CER forms (increased the [NP]/[NS] and [AP]/[AS] ratios), indicating it could reduce the relative feedback mechanism observed in inflammatory pathologies e.g., atopic dermatitis. The cream increased CER[NP], which is decreased in dry skin. Conclusion: G/P-emollient cream may be beneficial for skin pathologies by modifying SC lipids, balancing CER levels and ratios, and improving the barrier function. Importantly, the cream structure mimics that of the SC and penetrated the lower SC layers without compromising its lamellar structure.

show abstract

Exploratory in vivo biophysical studies of stratum corneum lipid organization in human face and arm skin

Cited by 4 publications

References 26 publications

A novel delivery vehicle for copper peptides

A novel delivery vehicle for copper peptides

An Investigation into the Transdermal Behavior of Active Ingredients by Combination of Experiments and Multiscale Simulations

Evaluation of a Novel Skin Emollient Cream on Skin Lipidome and Lipid Organization

Contact Info

Product

Resources

About