Exploration of Triticeae seed storage proteins for improvement of wheat end-product quality

Abstract

The seed storage proteins (SSPs) of cultivated wheat (Triticum aestivum and T. durum), namely, glutenin and gliadin, impart viscoelastic properties to bread dough, making wheat well suited for bread-making. Extensive studies on wheat SSPs have been carried out and revealed genetic diversity among wheat cultivars. Here, we review the studies of SSPs from more exotic species in the Triticeae tribe, primarily based on our own recent studies. SSPs of barley (Hordeum vulgare), homologous to those in wheat, exhibit quite different properties to wheat SSPs and do not produce viscoelastic dough. However, SSPs of a wild barley species (H. chilense) possess similar characteristics to the proteins of wheat. SSPs of rye (Secale cereale) substituted for wheat SSPs result in inferior quality. SSPs of Aegilops searsii and Ae. longissima have positive effects on quality, while Ae. umbellulata and Ae. geniculata (U g genome) SSPs have a negative effect on bread-making quality. SSPs located on chromosome 1M g of Ae. geniculata and a chromosome 1E of Thinopyrum elongatum have positive effects in addition lines but not in substitution lines for chromosome 1D of wheat. In contrast, SSPs of Th. intermedium have positive effects, even in cases of substitution for chromosome 1D of wheat, indicating promising potential for improvement of bread-making quality of wheat. Based on these results, we discuss the possibilities for utilizing the genetic variation of exotic Triticeae species for breeding improved quality traits in wheat.

Production of wheat-Psathyrostachys huashanica chromosome addition lines

“…Some of these lines have been adopted into practical breeding programs as materials for disease or insect resistance (Jiang et al, 1994;Friebe et al, 1996). Some other lines with single alien chromosomes may also be used for improvements in bread making quality, because some lines express novel seed storage proteins (Garg et al, 2009).…”

New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents

“…Genes located on chromosome 1V of Dasypyrum villosum in both addition (De Pace et al 2001) and translocation lines had also largely positive effects on bread-making quality. Furthermore, Garg et al (2009b) found that seed storage proteins of Th. intermedium had positive effects when transferred to wheat.…”

Potential Hybridization of Genetically Engineered Triticale with Wild and Weedy Relatives in Canada

“…Resistance to fungal and viral disease, drought and heat tolerance and its perennial nature has made Agropyron intermedium an attractive genetic donor species for bread wheat. Triticum ‐ Agropyron substitution lines have been developed and used but successful introgressions are rare because of undesirable gene linkages and instability of larger chromosome fragments (Friebe et al, 1996; Garg et al, 2008; Khan, 2000; Sibikeev et al, 1995). Mujeeb‐Kazi et al (1989) hand pollinated several varieties of bread wheat (Chinese Spring, Glennson 81, Nacozari‐76, and Pavon‐76) and A. intermedium in the field.…”