Exploration of the rumen microbial diversity and carbohydrate active enzyme profile of black Bengal goat using metagenomic approach

Suryawanshi, P. R.; Badapanda, Chandan; Singh, Krishna M.; Rathore, Ankita

doi:10.1080/10495398.2019.1609489

Cited by 5 publications

(9 citation statements)

References 37 publications

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“…The presence of archaeal organisms was confirmed in lactating goats grazing in plant communities in the Sonoran Desert but was marginal in both rumen (<0.02 % of total classified reads) and faeces (<0.09 %). These percentages of archaeal abundance were lower than those reported for the rumen of goats fed on grazing (0.17 %) [5], supplemented grazing (0.74–7.19 %) [47], and a mixed diet (0.59 %) [13]. A lower relative abundance of archaea could be caused by plant secondary metabolites abundant in scrublands [20, 21, 55].…”

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

“…Different studies have shown Bacteroidetes and Firmicutes interchange their place as the most abundant phylum in rumen bacteria of goats fed on native vegetation [46], mixtures of native vegetation and crops [4, 47], and mixtures of forage and concentrate [3, 13, 48–50]. Synergistetes , Verrumicrobia , Tenericutes , Proteobacteria , Euryarcheaota , and Actinocateria phyla also interchange their position as the third most abundant phylum, which is usually <8.0 % [4, 13, 47, 48]. However, Proteobacteria was the dominant phylum in the rumen of lactating goats grazing in native plant communities in the Sonoran Desert, followed by Firmicutes , and leaving Bacteroidetes in third place.…”

Section: Discussionmentioning

confidence: 99%

“…cellulose and starch), independent of mutualistic interactions with other members of the gastrointestinal microbiota [63]. This helps to understand their great abundance in the rumen bacterial community of lactating goats, whose circumstance is not common in goat rumen [3,4,[46][47][48]. In addition, Shen et al [50] found that Prevotella, Bacteroides (Bacteroidetes), and Clostridium (Firmicutes) genera may also contribute CAZyme-encoding genes in the digestive tract; the CAZyme are responsible for breaking down plant polysaccharides into their oligomers and monomers.…”

Section: Prokaryotic Communitiesmentioning

confidence: 99%

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Characterization of microbial communities from rumen and large intestine of lactating creole goats grazing in arid plant communities

et al. 2021

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Arid plant communities provide variable diets that can affect digestive microbial communities of free-foraging ruminants. Thus, we used next-generation sequencing of 16S and 18S rDNA to characterize microbial communities in the rumen (regurgitated digesta) and large intestine (faeces) and diet composition of lactating creole goats from five flocks grazing in native plant communities in the Sonoran Desert in the rainy season. The bacterial communities in the rumen and large intestine of the five flocks had similar alpha diversity (Chao1, Shannon, and Simpson indices). However, bacterial community compositions were different: a bacterial community dominated by Proteobacteria in the rumen transitioned to a community dominated by Firmicutes in the large intestine. Bacterial communities of rumen were similar across flocks; similarly occurred with large-intestine communities. Archaea had a minimum presence in the goat digestive tract. We detected phylum Basidiomycota, Ascomycota, and Apicomplexa as the main fungi and protozoa. Analyses suggested different diet compositions; forbs and grasses composed the bulk of plants in the rumen and forbs and shrubs in faeces. Therefore, lactating goats consuming different diets in the Sonoran Desert in the rainy season share a similar core bacterial community in the rumen and another in the large intestine and present low archaeal communities.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Prokaryotic Communitiesmentioning

confidence: 99%

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Characterization of microbial communities from rumen and large intestine of lactating creole goats grazing in arid plant communities

et al. 2021

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“…The ruminal (core) temperature in live goat is around 39.8°C (Szabuniewicz et al, 1972). Even though mesophiles are expected to predominate under normal conditions, there are reports on the metagenomic analysis of rumen microbial communities revealing the presence of numerous thermotolerant species belonging to bacterial phyla such as Bacteroidetes, Firmicutes, Proteobacteria, Euryarchaeota, Actinobacteria, Spirochaetes, Fusobacteria, Chloroflexi and Chlorobi (Suryawanshi et al, 2019). The microbiota of goat rumen can therefore serve as a unique source of thermostable cellulolytic and hemicellulolytic enzymes.…”

Section: Discussionmentioning

confidence: 99%

Thermoxylanolytic and Thermosaccharolytic Potential of a Heat Adapted Bacterial Consortium Developed From Goat Rumen Contents

et al. 2021

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Thermoactive xylanases have important applications in the industrial deconstruction of lignocellulosic plant biomass, due to their sustained activity even at high temperature conditions of industrial bioreactors. We herein report the development of a thermoactive xylanolytic microbial consortium from the semi-digested contents of goat rumen and characterization of the xylanolytic enzyme cocktail produced by it. The consortium exhibited maximum endoxylanase activity at pH6 and at 60°C. Zymogram analysis revealed the production of multiple xylanases. The xylanase cocktail was stable over a pH range of 5–9 after pre-incubation for 3 h. It retained 74% activity after pre-incubation (60°C) for 50 min. It’s activity was enhanced in presence of β-mercaptoethanol, NH4+, Mg2⁺ and Ca2⁺, whereas Hg2⁺ had an inhibitory effect. The xylanolytic cocktail was further utilized for the saccharification of alkali pre-treated rice straw and mushroom spent rice straw. Saccharification was studied quantitatively using the dinitrosalicylic acid method and qualitatively using scanning electron microscopy. Results indicated the potential of the xylanolytic cocktail for the saccharification of rice straw and highlighted the significance of chemical and/or biological pre-treatment in improving the accessibility of the substrate towards the xylanase cocktail.

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Understanding microbial networks of farm animals through genomics, metagenomics and other meta-omic approaches for livestock wellness and sustainability – A Review

Wani

Hashem

Akhtar

et al. 2022

Annals of Animal Science

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The association of microorganisms with livestock as endosymbionts, opportunists, and pathogens has been a matter of debate for a long time. Several livestock-associated bacterial and other microbial species have been identified and characterized through traditional culture-dependent genomic approaches. However, it is imperative to understand the comprehensive microbial network of domestic animals for their wellness, disease management, and disease transmission control. Since it is strenuous to provide a niche replica to any microorganisms while culturing them, thus a substantial number of microbial communities remain obscure. Metagenomics has laid out a powerful lens for gaining insight into the hidden microbial diversity by allowing the direct sequencing of the DNA isolated from any livestock sample like the gastrointestinal tract, udder, or genital system. Through metatranscriptomics and metabolomics, understanding gene expression profiles of the microorganisms and their molecular phenotype has become unchallenging. With large data sets emerging out of the genomic, metagenomic, and other meta-omics methods, several computational tools have also been developed for curation, assembly, gene prediction, and taxonomic profiling of the microorganisms. This review provides a detailed account of the beneficial and pathogenic organisms that dwell within or on farm animals. Besides, it highlights the role of meta-omics and computational tools in a comprehensive analysis of livestock-associated microorganisms.

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Exploration of the rumen microbial diversity and carbohydrate active enzyme profile of black Bengal goat using metagenomic approach

Cited by 5 publications

References 37 publications

Characterization of microbial communities from rumen and large intestine of lactating creole goats grazing in arid plant communities

Characterization of microbial communities from rumen and large intestine of lactating creole goats grazing in arid plant communities

Thermoxylanolytic and Thermosaccharolytic Potential of a Heat Adapted Bacterial Consortium Developed From Goat Rumen Contents

Understanding microbial networks of farm animals through genomics, metagenomics and other meta-omic approaches for livestock wellness and sustainability – A Review

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