Exploration of the role of the virulence factor ElrA during Enterococcus faecalis cell infection

Nunez, Natalia; Derré-Bobillot, Aurélie; Gaubert, Stéphane; Herry, Jean‐Marie; Deschamps, Julien; Yu, Wei; Baranek, Thomas; Si‐Tahar, Mustapha; Briandet, Romain; Serror, Pascale; Archambaud, Cristel

doi:10.1038/s41598-018-20206-6

Cited by 16 publications

(14 citation statements)

References 40 publications

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“…All samples were treated using a protocol similar to that described previously 34 . Briefly, individual intestinal segments from the SI, CA and CO were ligatured on both sides.…”

Section: Methodsmentioning

confidence: 99%

Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen

Archambaud

Derré-Bobillot

Lapaque

et al. 2019

Sci Rep

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Enterococci are subdominant members of the human gastrointestinal microbiota. Enterococcus faecalis is generally harmless for healthy individuals, but it can cause a diverse range of infections in immunodeficient or elderly patients with severe underlying diseases. In this study, we analysed the levels of intestinal translocation of indigenous enterococci in C57BL/6, CF-1 and CX3CR1 −/− mice upon clindamycin antibiotic-induced dysbiosis. We found that C57BL/6 was the most permissive model for enterococcal translocation and that initiation of E . faecalis translocation coincided with a threshold of enterococcal colonisation in the gut lumen, which once reached, triggered E . faecalis dissemination to deeper organs. We showed that the extent to which E . faecalis clinical strain VE14821 competed with indigenous enterococci differed between the C57BL/6 and CX3CR1 −/− models. Finally, using a simplified gnotobiotic model, we observed E . faecalis crossing an intact intestinal tract using intestinal epithelial cells as one route to reach the lamina propria. Our study opens new perspectives for assessing the effect of various immunodeficiencies and for investigating mechanisms underlying enterococcal translocation.

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“…All samples were treated using a protocol similar to that described previously 34 . Briefly, individual intestinal segments from the SI, CA and CO were ligatured on both sides.…”

Section: Methodsmentioning

confidence: 99%

Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen

Archambaud

Derré-Bobillot

Lapaque

et al. 2019

Sci Rep

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“…However, 100% nucleotide identity to the corresponding reference sequences was reported for only 6 of them -ElrA, SrtA, efaAfs, fsrB, gelE, and tpx. ElrA encodes an internalin-like protein that prevents the adhesion to macrophages (Nunez et al, 2018). StrA is a sortase enzyme that is essential for biofilm formation (Das et al, 2018) together with the fsrB (Gaspar et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

WGS Characterization of Enterococcus Faecalis H1041 Isolated From the Traditional Bulgarian Green Cheese

Dimov

Miteva²,

Dekova³

et al. 2022

J microb biotech food sci

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In this study, Enterococcus faecalis H1041 which was isolated from the traditional Bulgarian green cheese was characterized in depth by a whole-genome sequencing performed on the Illumina HiSeq platform using 2 x 150 bases paired-end reads mode. The bioinformatic analysis performed only with free online tools revealed that the strain resembles other cheese-born E. faecalis strains by its antibiotics’ resistances and virulence genotype, thus its presence within the green cheese should not be considered as a health risk. Only two antibiotic resistance genetic determinants were found, but they both carry mutations, and probably are not functional. Furthermore, 18 virulence factor genetic determinants were found, but only 6 of them possessed 100% identities with the canonical sequences, and being more or less intrinsic for the Enterococcus genus. None of them were found on contigs that contain sequences with known plasmid origin of replication. Enterolysin A gene was also detected which most probably plays role in ripening process of the Bulgarian green cheese.

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“…We previously observed that E. faecalis is internalized in HepG2 hepatic cells 28 . Considering increasing evidence that intestinal E. faecalis may be able to reach the liver of patients, this study examined the interactions between E. faecalis and hepatocytes, which account for 70% of hepatic cells in the liver, in more detail.…”

Section: Introductionmentioning

confidence: 95%

The unforeseen intracellular lifestyle of Enterococcus faecalis in hepatocytes

Nunez

Derré-Bobillot

Lakisic

et al. 2021

Preprint

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Enterococcus faecalis is a bacterial species present at a sub-dominant level in the human gut microbiota. This commensal turns into an opportunistic pathogen under specific conditions involving dysbiosis and host immune deficiency. E. faecalis is also the only intestinal pathobiont identified to date as contributing to liver damage in alcoholic liver disease. We have previously observed that E. faecalis is internalized in hepatocytes. Here, the survival and fate of E. faecalis was examined in hepatocytes, the main epithelial cell type in the liver. Although referred to as an extracellular pathogen, we demonstrate that E. faecalis is able to survive and divide in hepatocytes, and form intracellular clusters in two distinct hepatocyte cell lines, in primary mouse hepatocytes, as well as in vivo. This novel process extends to kidney cells. Unravelling the intracellular lifestyle of E. faecalis, our findings contribute to the understanding of pathobiont-driven diseases.

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Exploration of the role of the virulence factor ElrA during Enterococcus faecalis cell infection

Cited by 16 publications

References 40 publications

Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen

Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen

WGS Characterization of Enterococcus Faecalis H1041 Isolated From the Traditional Bulgarian Green Cheese

The unforeseen intracellular lifestyle of Enterococcus faecalis in hepatocytes

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