Exploration of the direct metabolic effects of mercury II chloride on the kidney of Sprague–Dawley rats using high-resolution magic angle spinning 1H NMR spectroscopy of intact tissue and pattern recognition

Wang, Yulan; Bollard, Mary E.; Nicholson, Jeremy K.; Holmes, Elaine

doi:10.1016/j.jpba.2005.07.019

Cited by 36 publications

(15 citation statements)

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“…Further, insulin released from injured acinar cells also contributed to decreased glucose levels. TMAO, the product of dietary choline and L-carnitine [28] , although mainly regarded as a biomarker of renal function [29], may participate in energy production as an external electron acceptor in an hypoxic environment[30]. Moreover, decreased TMAO has been reported to accompany a chemiosmotic mechanism of energy conversion [31].…”

Section: Discussionmentioning

confidence: 99%

1HNMR-based metabolomic profile of rats with experimental acute pancreatitis

Zhao

Liu

et al. 2014

BMC Gastroenterol

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BackgroundAcute pancreatitis (AP) is a common inflammatory disease of the pancreas accompanied by serious metabolic disturbances. Nevertheless, the specific metabolic process of this disease is still unclear. Characterization of the metabolome may help identify biomarkers for AP. To identify potential biomarkers, this study therefore investigated the 1H-nuclear magnetic resonance (NMR)-based metabolomic profile of AP.MethodsFourteen male adult Sprague–Dawley rats were randomized into two groups: the AP group, in which AP was induced by retrograde ductal infusion of 3.5% sodium taurocholate; and the sham operation group (SO), in which rats were infused with 0.9% saline. Blood samples were obtained 12 hours later and a 600 MHz superconducting NMR spectrometer was used to detect plasma metabolites. Principal components analysis (PCA) and partial least squares-discriminant analysis after orthogonal signal correction (OSC-PLS-DA) were used to analyze both longitudinal Eddy-delay (LED) and Carr–Purcell–Meiboom–Gill (CPMG) spectra.ResultsDifferences in plasma metabolites between the two groups were detected by PCA and PLS-DA of 1HNMR spectra. Compared with the SO group, plasma levels of lactate (δ 1.3, 1.34, 4.1), valine (δ 0.98, 1.02), succinic acid (δ 2.38), 3-hydroxybutyric acid (3-HB, δ 1.18), high density lipoprotein (HDL, δ 0.8), and unsaturated fatty acid (UFA, δ 2.78, 5.3) were elevated in the AP group, while levels of glycerol (δ 3.58, 3.66), choline (δ 3.22), trimethylamine oxide (TMAO, δ 3.26), glucose (δ 3–4), glycine (δ 3.54), very low density lipoprotein (VLDL, δ 1.34) and phosphatidylcholine (Ptd, δ 2.78) were decreased.ConclusionsAP has a characteristic metabolic profile. Lactate, valine, succinic acid, 3-HB, HDL, UFA, glycerol, choline, TMAO, glucose, glycine, VLDL, and Ptd may be potential biomarkers of early stage AP.

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Section: Discussionmentioning

confidence: 99%

1HNMR-based metabolomic profile of rats with experimental acute pancreatitis

Zhao

Liu

et al. 2014

BMC Gastroenterol

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“…[20] If the damage caused by the mercury is very intensive, the kidneys are likely to recover once the body clears itself of the contamination. [21] Mercury induces acute renal failure associated to tubular impairment in experimental animals and humans. [22] Also a lot of studies revealed that mercury is highly toxic metal that induces oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Mercury-induced alterations in rat kidneys and testes in vivo

Massányi

Lukáč

Slivkova

et al. 2007

Journal of Environmental Science and Health, Part A

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In this study effects of mercury administration on the kidney and testicular structure of adult rats were evaluated. Rats received mercury (HgCl2) in single intraperitoneal dose 20 mg HgCl2 (group A), 10 mg HgCl2 (group B) and 5 mg HgCl2 (group C) per kilogram of body weight and were killed after 48 hours following mercury administration. After the preparation of histological samples the results were compared with control group (K). In kidney decreased diameters of glomeruli and renal corpuscles, damaged tubules with affected quality of tubular cells and infiltration of interstitium were detected. Quantitative analysis demonstrated increased relative volume of tubules and renal corpuscles. Also the number of nuclei and glomeruli was increased in all experimental groups. The diameter of glomeruli and renal corpuscles was decreased. In testis undulation of basal membrane, dilatation of blood vessels in interstitium and occurrence of empty spaces in germinal epithelium were observed. Decreased relative volume of germinal epithelium, increased relative volume of interstitium and increased apoptosis occurrence suggest damaged interstitium and revealed occurrence of edemas. The relative volume of seminiferous tubules showed higher luminization. The number of nuclei was decreased in all experimental groups what is in positive relation with occurrence of empty spaces. Also other evaluated criteria demonstrated significant differences between control group and experimental groups. This study reports a negative effect of mercury on the structure and function of kidney and testes.

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“…These endogenous metabolites were selected as putative biomarkers for HgCl 2 -induced nephrotoxicity. Increased glutamate was present in the renal cortex and may be associated with HgCl 2 -induced renal acidosis and disruption of the TCA cycle [71]. In contrast, 2-oxoglutarate, citrate, formate, glucose, and taurine were significantly changed only at higher HgCl 2 .…”

Section: Environmental Pollutantsmentioning

confidence: 95%