Exploration of the anti-hyperuricemia effect of TongFengTangSan (TFTS) by UPLC-Q-TOF/MS-based non-targeted metabonomics

Huang, Zhichao; Zhang, Wugang; An, Qi; Lang, Yifan; Liu, Ye; Fan, Huifang; Chen, Haifang

doi:10.1186/s13020-023-00716-w

Cited by 8 publications

(5 citation statements)

References 52 publications

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“…The possible pathways for TAP affecting PO-induced HUA were “neuroactive ligand–receptor interaction”, “purine metabolism”, “protein digestion and absorption”, “propanoate metabolism”, and “ABC transporters”, among which purine metabolism had the highest number of metabolites and the highest enrichment significance. It was suggested that TAP mainly regulated purine metabolism to alleviate HUA, corresponding to the previous results. ,, Besides, the difference abundance score map of the pathway (Figure B) also confirmed that the downregulation of purine metabolism and ABC transporters contributed to the effect of HUA.…”

Section: Resultssupporting

confidence: 84%

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Identification of Hyperuricemia Alleviating Peptides from Yellow Tuna Thunnus albacares

Hao,

Ding,

Fan

et al. 2024

J. Agric. Food Chem.

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The development of food-derived antihyperuricemic substances is important for alleviating hyperuricemia (HUA) and associated inflammation. Here, novel peptides fromThunnus albacares (TAP) with strong antihyperuricemic activity were prepared. TAP was prepared by alkaline protease (molecular weight <1000 Da), with an IC 50 value of xanthine oxidase inhibitory activity of 2.498 mg/mL, and 5 mg/mL TAP could reduce uric acid (UA) by 33.62% in human kidney-2 (HK-2) cells (P < 0.01). Mice were fed a high-purine diet and injected with potassium oxonate to induce HUA. Oral administration of TAP (600 mg/kg/d) reduced serum UA significantly by 42.22% and increased urine UA by 79.02% (P < 0.01) via regulating urate transporters GLUT9, organic anion transporter 1, and ATP-binding cassette subfamily G2. Meantime, TAP exhibited hepatoprotective and nephroprotective effects, according to histological analysis. Besides, HUA mice treated with TAP showed anti-inflammatory activity by decreasing the levels of toll-like receptor 4, nuclear factors-κB p65, NLRP3, ASC, and Caspase-1 in the kidneys (P < 0.01). According to serum non-targeted metabolomics, 91 differential metabolites between the MC and TAP groups were identified, and purine metabolism was considered to be the main pathway for TAP alleviating HUA. In a word, TAP exhibited strong antihyperuricemic activity both in vitro and in vivo.

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Section: Resultssupporting

confidence: 84%

“…It was suggested that TAP mainly regulated purine metabolism to alleviate HUA, corresponding to the previous results. 36,53,58 Besides, the difference abundance score map of the pathway (Figure 8B) also confirmed that the downregulation of purine metabolism and ABC transporters contributed to the effect of HUA.…”

Section: Effects Of Tap On Serum Andsupporting

confidence: 52%

Identification of Hyperuricemia Alleviating Peptides from Yellow Tuna Thunnus albacares

Hao,

Ding,

Fan

et al. 2024

J. Agric. Food Chem.

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“…The dry extract was then reconstituted in 1 mL methanol, and centrifuged for 10 min at 14 000 g at 4 °C. The supernatant was then filtered and subjected to LC–MS analysis [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

Integrating animal experiments, mass spectrometry and network-based approach to reveal the sleep-improving effects of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

Ren,

Wu,

Wang

et al. 2023

Chin Med

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Background Ziziphi Spinosae Semen (ZSS) is a plant widely used as medicine and food in Asian countries due to its numerous health benefits. γ-aminobutyric acid (GABA), a non-proteinaceous amino acid, is one of the major inhibitory neurotransmitters with a relaxant function. In this study, a system pharmacology approach was employed to assess the effects of a mixture composed of ZSS and GABA (ZSSG) on sleep improvement. Methods Mice were divided into five groups (n = 10) and received either no treatment, sodium pentobarbital, or sodium barbital with diazepam or ZSSG. The effects of ZSSG on sleep quality were evaluated in mice, and differential metabolites associated with sleep were identified among the control, ZSS, GABA, and ZSSG groups. Additionally, network-based ingredient-insomnia proximity analysis was applied to explore the major ingredients. Results ZSSG significantly improved sleep quality by decreasing sleep latency and prolonging sleep duration in sodium pentobarbital-induced sleeping mouse model (P < 0.05). ZSSG significantly enhanced the brain content of GABA in mice. Furthermore, ZSSG also significantly decreased sleep latency-induced by sodium barbital in mice (P < 0.05). Metabolic analysis revealed significant differences in 10 metabolites between ZSSG group and the groups administering ZSS or GABA. Lastly, using the network-based ingredient screening model, we discovered potential four active ingredients and three pairwise ingredient combinations with synergistic effect on insomnia from ZSSG among 85 ingredients identified by UPLC-Q/TOF–MS. Also, we have constructed an online computation platform. Conclusion Our data demonstrated that ZSSG improved the sleeping quality of mice and helped to balance metabolic disorders-associated with sleep disorders. Moreover, based on the network-based prediction method, the four potential active ingredients in ZSSG could serve as quality markers-associated with insomnia. The network-based framework may open up a new avenue for the discovery of active ingredients of herbal medicine for treating complex chronic diseases or symptoms, such as insomnia.

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“…The dry extract was then reconstituted in 1 mL methanol, and centrifuged for 10 min at 14 000 g at 4°C. The supernatant was then ltered and subjected to LC-MS analysis [12].…”

Section: Animal Treatment and Collection Of Brain Samples For Metabol...mentioning

confidence: 99%

Integrating animal experiments, mass spectrometry and network-based approach to reveal the improving sleep quality of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

Cui

Ren

et al. 2023

Preprint

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Background Ziziphi Spinosae Semen (ZSS) is a plant widely used as medicine and food in Asian countries due to its numerous health benefits. γ-aminobutyric acid (GABA), a non-proteinaceous amino acid, is one of the major inhibitory neurotransmitters with a relaxant function. In this study, a systems pharmacology approach was employed to assess the effects of a mixture composed of ZSS and GABA (ZSSG) on sleep improvement.Methods Mice were divided into five groups (n = 10) and received either no treatment, sodium pentobarbital, or sodium barbital with diazepam or ZSSG. The effects of ZSSG on sleep quality were evaluated in mice, and differential metabolites associated with sleep were identified among the control, ZSS, GABA, and ZSSG groups. Additionally, a network-based ingredient-insomnia proximity analysis was applied to explore the major ingredients.Results ZSSG significantly improved sleep quality by decreasing sleep latency and prolonging sleep duration in sodium pentobarbital-induced sleeping mouse model (P < 0.05). ZSSG significantly enhanced the brain content of GABA in mice. Furthermore, ZSSG also significantly decreased sleep latency-induced by sodium barbital in mice (P < 0.05). Metabolic analysis revealed significant differences in 10 metabolites between ZSSG group and the groups administering ZSS or GABA. Lastly, using the network-based ingredient screening model, we discovered potential four active ingredients and three pairwise ingredient combinations with synergistic effect for insomnia from ZSSG among 85 ingredients identified by UPLC-Q/TOF-MS. Also, we have constructed an online computation platform.Conclusion Our data demonstrated that ZSSG improved the sleeping quality of mice and help to balance metabolic disorders-associated with sleep disorders. Moreover, based on the network-based prediction method, the potential four active ingredients in ZSSG could serve as quality markers-associated with insomnia. The network-based framework may open up a new avenue for the discovery of active ingredients of herbal medicine for treating complex chronic diseases or symptom, such as insomnia.

show abstract

Exploration of the anti-hyperuricemia effect of TongFengTangSan (TFTS) by UPLC-Q-TOF/MS-based non-targeted metabonomics

Cited by 8 publications

References 52 publications

Identification of Hyperuricemia Alleviating Peptides from Yellow Tuna Thunnus albacares

Identification of Hyperuricemia Alleviating Peptides from Yellow Tuna Thunnus albacares

Integrating animal experiments, mass spectrometry and network-based approach to reveal the sleep-improving effects of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

Integrating animal experiments, mass spectrometry and network-based approach to reveal the improving sleep quality of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

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