Exploration of the 1-deoxy-d-xylulose 5-phosphate synthases suitable for the creation of a robust isoprenoid biosynthesis system

Kudoh, Kai; Kubota, Genma; Fujii, Rintaro; Kawano, Yusuke; Ihara, Masaki

doi:10.1016/j.jbiosc.2016.10.005

Cited by 14 publications

(14 citation statements)

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“…DXP is not only precursor for subsequent MEP pathway reaction but also for thiamine (vitamin B1) and pyridoxol (vitamin B6) biosynthesis (Hill et al, 1989;Wagner et al, 2000;Hazra et al, 2009). Several studies indicate that formation of DXP is the limiting step of the MEP pathway (Julsing et al, 2007;Zhao et al, 2011;Banerjee et al, 2013;Hess et al, 2013;Banerjee & Sharkey, 2014;Kudoh et al, 2017). Suppression of the gene impaired the growth of the bacterium shown by its small colony and reduced isoprene emission and full suppression of the gene led to lethality (Julsing et al, 2007).…”

Section: First Step Of the Mep Pathway: Linking The Terpenoid And Cenmentioning

confidence: 99%

“…Negative feedback imposed by IDP and DMADP is natural to Dxs and exists across species. Nevertheless, a study comparing several different Dxs enzymes found that B. subtilis Dxs is more resistant to feedback inhibition compared to E. coli and other bacteria (Kudoh et al, 2017). B. subtilis Dxs is also considered to be more resistant to proteases as compared to Dxs from E. coli, Paracoccus aminophilus and Rhodobacter capsulatus.…”

Section: Accepted Articlementioning

confidence: 99%

“…However, it came with cost of higher K M of ThDP and pyruvate that overall decreased the catalytic efficiency of the enzyme about 15 times compared to the wild type (Banerjee et al, 2016). B. subtilis Dxs has been found to be more resistant to negative feedback of IDP/ DMADP but it has higher K M compared to Dxs of E. coli (five times higher for G3P and three times higher for pyruvate) (Kudoh et al, 2017). Yet, expression of B. subtilis Dxs in E. coli produced higher amount of isoprene compared to Dxs of other microorganisms including E. coli counterpart after 24 hours of incubation.…”

Section: Protein Engineeringmentioning

confidence: 99%

Section: First Step Of the Mep Pathway: Linking The Terpenoid And Central Carbon Pathwaymentioning

confidence: 99%

“…). Bacillus subtilis Dxs has been found to be more resistant to negative feedback of IDP/ DMADP but it has higher K M compared to Dxs of E. coli (five times higher for G3P and three times higher for pyruvate) (Kudoh et al 2017…”

Section: Current Genetic Engineering Tools and Promising Strategies To Improve Terpenoid Production In B Subtilismentioning

confidence: 99%

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Positioning Bacillus subtilis as terpenoid cell factory

et al. 2020

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Increasing demands for bioactive compounds have motivated researchers to employ microorganisms to produce complex natural products. Currently, Bacillus subtilis has been attracting lots of attention to be developed into terpenoids cell factories due to its generally recognized as safe status and high isoprene precursor biosynthesis capacity by endogenous methylerythritol phosphate (MEP) pathway. In this review, we describe the up-to-date knowledge of each enzyme in MEP pathway and the subsequent steps of isomerization and condensation of C5 isoprene precursors. In addition, several representative terpene synthases expressed in B. subtilis and engineering steps to improve corresponding terpenoids production are systematically discussed. Furthermore, the current available genetic tools are mentioned as well as promising strategies to improve terpenoids in B. subtilis, hoping to inspire future directions in metabolic engineering of B. subtilis for further terpenoids cell factory development.

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