Exploration of Phytoconstituents from Mussaenda roxburghii and Studies of Their Antibiofilm Effect

Abstract

In the context of ethno botanical importance with no phytochemical investigations, Mussaenda roxburghii have been investigated to explore it's phytoconstituents and studies of their antibiofilm activity. Four compounds have been isolated from the aerial parts of this plant and were characterized as 2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid (1), β-sitosterol glucoside (4), lupeol palmitate (5), and myoinositol (6). All these compounds were tested for antibacterial and antibiofilm activity against Pseudomonas aeruginosa. Compound 1 exhibited three times more antibiofilm activity with minimum inhibitory concentration (MIC) at 0.74 mm compared to that of streptomycin. Molecular docking studies exhibited a very high binding affinity of 1 with P. aeruginosa quorum sensing proteins and motility associated proteins viz. LasR and PilB, PilY1, PilT, respectively. Compound 1 was also found to be non-cytotoxic against sheep RBC and murine peritoneal macrophages at selected sub-MIC doses.

Anti-breast-Cancer Activity Exerted by β-Sitosterol-d-glucoside from Sweet Potato via Upregulation of MicroRNA-10a and via the PI3K–Akt Signaling Pathway

“…The results revealed that the new compound was β-sitosterol-D-glucoside (β-SDG). 25 β-SDG Inhibition of BC-Cell Proliferation in Vitro. To assess what happens upon β-SDG treatment, the MCF7 and MDA-MB-231 BC cell lines as well as the control normal MCF-10A breast cell line were treated for 24 h with various doses of β-SDG, and MTT and colony-formation assays were used to quantify cell viability and proliferation, respectively.…”

“…Cytoplasmic cytochrome c release is a key step of the mitochondria-mediated apoptotic pathway regulated by the Bcl-2 protein family. 25 We thus assessed expression of cytochrome c and the Bcl-2 family, which consists of members that either promote or inhibit apoptosis. 26 As shown in Figure 6C, β-SDG significantly elevated proapoptotic Bax and Bad levels, and simultaneously reduced the antiapoptotic Bcl-2 and Bcl-xl levels, and cytochrome c expression was also upregulated by β-SDG (Figure S5).…”

“…The purity of this compound was 98.0% as analyzed by HPLC, and its structure was identified from 1 H NMR (Figure S1) and 13 C NMR (Figure S2) spectra (Bruker DRX 500 spectrometer) on the basis of a reference of residual pyridine- d 5 . The results revealed that the new compound was β-sitosterol- d -glucoside (β-SDG) …”

Plant Derived Natural Products against Pseudomonas aeruginosa and Staphylococcus aureus: Antibiofilm Activity and Molecular Mechanisms

“…The latter diffuses toward the surrounding cells initiating QS, interacts lasR with the transcription factor, and activates multiple virulence genes, including lasB. In silico docking studies with proteins, like the las family (lasA, lasI, and lasR), luxR, and pil family (pilB, pilT, and pilY1), showed that tormentic and 23-hydroxycorosolic acids [40], as well as a third ursane triperpene, 23-hydroxytormentic acid from Mussaenda roxburghii [41], have good binding affinity with all the selected proteins.…”

Studies on Toxicological and Neurobehavioral Profile of Methanol Extract of Mussaenda Roxburghii Hook. F. Leaves in Mice

“…The study on albino mice reported MR at the dose of 600 mg/kg produce toxicity concerning food intake, body weight (BWT), and general appearances [6]. The phytochemical analysis has reported that Mussaenda genus presents active phytochemical iridoids, triterpenes, and flavonoid and also, other phytochemicals such as beta-sitosterol glucosides, lupeol palmitate, and myoinositol [7,8]. Dietary sterol glucosides were neurotoxic to motor neurons [9].…”