Exploration of Molecular Variability in Rhizoctonia Bataticola, the Incitant of Root Rot Disease of Pulse Crops

Abstract

Abatract:The present study explored pathogenic and genetic variability among the eleven isolates of Rhizoctonia bataticola (Taub.) Butler from different pulse crops. Based on morphological characters, 11 isolates were categorized into three groups viz., linear, fluffy, and linear at the end with fluffy growth at the center. Isolates also showed variability in sclerotial characters (intensity and shape) and intensity of pigment synthesis. All isolates were more aggressive on the original host from which it was isolated, which was shown by the variability in pathogenic characters. RAPD-PCR analysis has shown that genetic clustering agreed with the above findings in dendrogram analysis (2 clusters A and B). The black gram root isolates showed a maximum genetic similarity of 73 % with soya bean shoot isolate. Red gram shoot isolate showed 61% genetic similarity with green gram isolates. The findings from this study confirm the variability in R. bataticola isolates from pulses, according to their pathological as well as genetic characters. In the future, variability in pathogens will determine effective management practices.

Characterization of molecular variability in Rhizoctonia solani isolates from different agro-ecological zones by random amplified polymorphic DNA (RAPD) markers

“…RAPD-PCR has been successfully used for studying inter and intra-specific variability among populations from different as well as the same geographical regions (Sundravadana et al, 2011). The results of our present study indicate significant level of variation among the 22 isolates of R. solani.…”

“…Perhaps, evaluation of the genetic diversity in pathogen isolates has been an initial step towards understanding the population structure. Molecular techniques have become reliable and are highly suitable tools for identifying pathogen species and for assessing genetic variation within collections and populations (Sundravadana et al, 2011). With the advent of various molecular marker technologies, the studies of genetic diversity in plant pathogens have become feasible.…”

Studies on Characterization of Molecular Variability Using RAPD Markers in Rhizoctonia solani Isolated from Different Gerographical Regions of South India

“…The use of molecular marker to study the genetic diversity relationships among populations from different as well as the same geographical regions (Sundravadana et al, 2011).…”

“…Diverse Rice sheath blight isolates has been studied by morphological characterization and pathogenicity testing and also by various molecular techniques reliable and are highly suitable tools for identifying pathogen species and analyzing the genetic variations of microorganisms (Sundravadana et al, 2011). RAPD (Random Amplified Polymorphic DNA) markers have been successfully used to determine the numerous filamentous fungi species collected from different fields of experimental mycology (Pollastro et al, 2000).…”

Diversity analysis of Rhizoctonia solani causing sheath blight of rice in India

“…Molecular techniques have become reliable and are highly suitable tools for identifying pathogen species and for assessing genetic variation within collections and populations (Sundravadana et al, 2011). Recently, Banerjee et al (2012) reported that the RAPD can still be considered as a reliable, efficient and effective marker technology for determining genetic relationships in Rhizoctonia spp.…”