Exploration of highly selective fluorogenic ‘on–off' chemosensor for H₂PO₄⁻ ions: ICT‐based sensing and ATPase activity profiling

Abstract

In this study, the recognition contour of Chemosensor 1 was investigated using semiaqueous methanol (X H , mole fraction = 0.31) for a range of anions and bioactive species. Host-receptor signalling based on the internal charge transfer mechanism for Chemosensor 1 was explored and reported. Structure of Chemosensor 1 and its plausible anion coordination based on hydrogen bonding is complemented with density functional theory. Consequently, we investigated the applicability of the synthesized probe in blood plasma, urine, tap water samples, and for monitoring of ATP in lysosomes by apyrase enzyme. K E Y W O R D Sanion coordination, apyrase activity, ATPase activity, ICT-based chemosensor, quenching

A Highly Selective and Sensitive Sequential Recognition Probe Zn2+ and H2PO4− Based on Chiral Thiourea Schiff Base

“…In recent years, widespread research has been undertaken on the detection of metal ions [ 1 , 2 , 3 , 4 , 5 ] and inorganic anions [ 6 , 7 , 8 , 9 ], particularly within the development of selective ion probes, which has more generally become a popular topic in scientific research. Zn 2+ is the second most common element in the human body, after iron, and not only plays an important role in protein structure, catalysis, transcription, and regulation, but is also closely associated with nerve signal transmission, enzyme regulation, and gene expression [ 10 ].…”

“…Phosphates can regulate the level of 1,25-(OH) 2-vitamin D in human plasma [ 19 ] and react with hydroxyl groups to form phosphate esters, thus allowing monomers to polymerize into relatively stable long chain skeletons (DNA and RNA skeletons) [ 20 ]. In addition, phosphate is also involved in ATP energy supply, ion channel regulation, enzymatic reactions, and intercellular signal transduction [ 8 ]. Unfortunately, a large amount of phosphate deposition can lead to a lack of dissolved oxygen in water and algae eutrophication [ 18 ].…”

“…Molecules 2023, 28, x FOR PEER REVIEW 2 of 14 regulation, enzymatic reactions, and intercellular signal transduction [8]. Unfortunately, a large amount of phosphate deposition can lead to a lack of dissolved oxygen in water and algae eutrophication [18].…”