Exploration of Genetic Variants within the Goat A-Kinase Anchoring Protein 12 (AKAP12) Gene and Their Effects on Growth Traits

Bai, Yangyang; Yuan, Rongrong; Luo, Yunyun; Kang, Zihong; Zhu, Haijing; Liu, Qu; Lan, Xianyong; Song, Xiaoling

doi:10.3390/ani11072090

Cited by 6 publications

(7 citation statements)

References 44 publications

(55 reference statements)

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“…45,46 AKAP12, a scaffolding protein gene affecting multiple signaling effectors, has been shown to play a significant role in regulating growth and development. 47 Studies have shown that it is related to inflammation, however, the underlying mechanism is not clear. 48 osteogenesis-inducing conditions, which is consistent with the results of bioinformatics analysis.…”

Section: Discussionmentioning

confidence: 99%

“…PLAT, an ECM remodeling enzyme, has been observed to function during the recovery phase of colitis 45,46 . AKAP12, a scaffolding protein gene affecting multiple signaling effectors, has been shown to play a significant role in regulating growth and development 47 . Studies have shown that it is related to inflammation, however, the underlying mechanism is not clear 48 .…”

Section: Discussionmentioning

confidence: 99%

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Identifying genes for regulating osteogenic differentiation of human periodontal ligament stem cells in inflammatory environments by bioinformatics analysis

Wang,

Yang,

Wang

et al. 2023

J of Periodontal Research

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Background and ObjectivesPeriodontitis is an immuno‐inflammatory disease caused by dental plaque biofilms and inflammations. The regeneration of bone tissue in inflammatory environment is of great significance for the treatment of periodontal disease, but the specific molecular mechanism of bone formation in periodontitis still needs further exploration. The objective of this study was to identify key osteogenesis‐related genes (ORGs) in periodontitis.MethodsWe used two datasets from the Gene Expression Omnibus (GEO) database to find differentially expressed mRNAs and miRNAs, further performed Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Then we predicted the downstream genes of the differentially expressed miRNAs (DEMs) by the TargetScan database and established a miRNA‐mRNA regulatory network. Finally, the osteogenic mechanism of periodontitis was explored through quantitative real‐time PCR (qRT‐PCR) by inducing inflammatory environment and osteogenic differentiation of hPDLSCs.ResultsThrough differential expression analysis and prediction of downstream target genes of DEMs, we created a miRNA‐mRNA regulatory network consisting of 29 DEMs and 11 differentially expressed osteogenesis‐related genes (DEORGs). In addition, the qRT‐PCR results demonstrated that BTBD3, PLAT, AKAP12, SGK1, and GLCE expression levels were significantly upregulated, while those of TIMP3, ZCCHC14, LIN7A, DNAH6, NNT, and ITGA6 were downregulated under the dual effects of inflammatory stimulation and osteogenic induction.ConclusionDEORGs might be important factors in the osteogenic phase of periodontitis, and the miRNA‐mRNA network may shed light on the clarification of the role and mechanism of osteogenesis in periodontitis and contribute to the development of novel therapeutic strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identifying genes for regulating osteogenic differentiation of human periodontal ligament stem cells in inflammatory environments by bioinformatics analysis

Wang,

Yang,

Wang

et al. 2023

J of Periodontal Research

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“…As one of the most important economic traits in large-scale breeding, goat reproductive traits account for a large proportion of the overall income of breeders. However, the low heritability of reproductive traits, the long cycle, the slow breeding progress and the complex relationship with growth traits are the main reasons for the economic disadvantages of goat breeding [ 34 , 35 ]. With the development of biotechnology, marker-assisted selection (MAS) has been widely used in goat breeding to rapidly enhance goat reproductive performance.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Copy Number Variations (CNVs) of the Goat PPP3CA Gene and Their Effect on Litter Size and Semen Quality

Bai

Zhang

Liu

et al. 2022

Animals

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Copy number variations (CNVs) have many forms of variation structure, and they play an important role in the research of variety diversity, biological evolution and disease correlation. Since CNVs have a greater impact on gene regulation and expression, more studies are being finalized on CNVs in important livestock and poultry species. The protein phosphatase 3 catalytic subunit alpha (PPP3CA) is a key candidate gene involved in the goat fecundity trait, and has important effects on precocious puberty, estrogen signal transduction pathways and oocyte meiosis. Additionally, PPP3CA also has a dephosphorylation effect in the process of spermatogonial stem cell meiosis and spermatogenesis. So far, there is no research on the relationship between the copy number variations of the PPP3CA gene and reproduction traits. Therefore, the purpose of this study was to determine the association between copy number variations in the goat PPP3CA gene and litter size and semen quality in Shaanbei white cashmere goats (SBWC) (n = 353) and Guizhou Heima goats (n = 64). Based on the association analysis, the results showed that only CNV1 and CNV2 within the PPP3CA gene were distinctly related to the first-birth litter size in female goats (p = 7.6802 × 10−11; p = 5.0895 × 10−9, respectively) and they were also significantly associated with the semen quality of SBWC goats (p < 0.05). In addition, individuals with Loss genotypes demonstrated better phenotypic performance compared to those with other types. Therefore, CNV1 and CNV2 of the PPP3CA gene are potentially useful for breeding, as they are linked to important goat reproduction traits.

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“…InDel variants in introns should not be ignored; many InDels located in intron regions of genes significantly affect animal production traits. For example, variants in the introns of SNX29 [ 16 ], IGF2BP [ 14 ], and AKAP12 [ 44 ] significantly affect body traits in animals. Similar to the results of the previous studies, the three InDel loci in this trial were also located in introns, and they significantly correlated with body traits.…”

Section: Discussionmentioning

confidence: 99%

Relationships between the Mini-InDel Variants within the Goat CFAP43 Gene and Body Traits

Wang

et al. 2022

Animals

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The cilia- and flagella-associated protein 43 (CFAP43) gene encodes a member of the cilia- and flagellum-associated protein family. Cilia on the cell surface influence intercellular signaling and are involved in biological processes such as osteogenesis and energy metabolism in animals. Previous studies have shown that insertion/deletion (InDel) variants in the CFAP43 gene affect litter size in Shaanbei white cashmere (SBWC) goats, and that litter size and body traits are correlated in this breed. Therefore, we hypothesized that there is a significant relationship between InDel variants within the CFAP43 gene and body traits in SBWC goats. Herein, we first investigated the association between three InDel variant loci (L-13, L-16, and L-19 loci) within CFAP43 and body traits in SBWC goats (n = 1827). Analyses revealed that the L-13, L-16, and L-19 loci were significantly associated with chest depth, four body traits, and three body traits, respectively. The results of this study are in good agreement with those previously reported and could provide useful molecular markers for the selection and breeding of goats for body traits.

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Exploration of Genetic Variants within the Goat A-Kinase Anchoring Protein 12 (AKAP12) Gene and Their Effects on Growth Traits

Cited by 6 publications

References 44 publications

Identifying genes for regulating osteogenic differentiation of human periodontal ligament stem cells in inflammatory environments by bioinformatics analysis

Identifying genes for regulating osteogenic differentiation of human periodontal ligament stem cells in inflammatory environments by bioinformatics analysis

Investigation of Copy Number Variations (CNVs) of the Goat PPP3CA Gene and Their Effect on Litter Size and Semen Quality

Relationships between the Mini-InDel Variants within the Goat CFAP43 Gene and Body Traits

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