Exploration of genes showing intramuscular fat deposition‐associated expression changes in musculus longissimus muscle

Sasaki, Yoshiyuki; Nagai, Koutatsu; Nagata, Yukihiro; Doronbekov, K; Nishimura, Syoyo; Yoshioka, Satoru; Fujita, Tatsuo; Shiga, Kazuho; Miyake, Teruki; Taniguchi, Yoshinori; Yamada, Takahisa

doi:10.1111/j.1365-2052.2005.01380.x

Cited by 87 publications

(76 citation statements)

References 16 publications

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“…BTG2 belongs to the anti-proliferative gene family and has been shown to be involved in cell growth, differentiation and survival (Mo et al 2011), muscle fibre size, intramuscular fat deposition and weight loss (Kamaid and Giráldez 2008;Sasaki et al 2006). FA analyses demonstrated that x-3 and x-6 FAs increased in the liver of lambs supplemented at 10 and 20 % Spirulina levels.…”

Section: Discussionmentioning

confidence: 96%

Molecular genetics-nutrition interactions in the expression of AANAT, ADRB3, BTG2 and FASN genes in the heart, kidney and liver of Australian lambs supplemented with Spirulina (Arthrospira platensis)

Malau‐Aduli

Kashani

2015

Genes Genom

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Quantitative reverse transcriptase polymerase chain reaction was used to assess the messenger RNA (mRNA) transcription levels and expressions of Aralkylamine N-acetyltransferase, Adrenergic beta-3 receptor, B cell translocation gene 2, and Fatty acid synthase genes. In total, 432 heart, kidney and liver samples from 48 purebred and crossbred Australian prime lambs supplemented with Spirulina (Arthrospira platensis) blue-green cyanobacterial microalgae over a 9-week feeding trial were utilized for fatty acid composition and mRNA extraction. The aim was to assess the effect of dietary Spirulina supplementation on the mRNA expression profiles of genes controlling fatty acid metabolism in the heart, kidney and liver. Fourty-eight weaned lambs belonging to four breeds under the same management conditions were maintained on ryegrass pasture and randomly allocated to three treatment groups that comprised the control and two levels of Spirulina supplementation: Low (100 mL/head/day of 1 g Spirulina powder dissolved in 10 mL of water (10 % wt:vol)) and High (200 mL/head/day of 2 g of Spirulina dissolved in 10 mL of water (20 % wt:vol)). Both the low and high levels of Spirulina supplementation regimes strongly up-regulated the transcription of all the selected genes and increased x-3 and x-6 polyunsaturated fatty acid compositions in the tested organs. Breed and sex did not influence gene expression patterns; however, significant variations in response to Spirulina supplementation underpin the genetics-nutrition interactions that could be of practical importance for manipulating meat quality in the Australian dual-purpose prime lamb industry for a healthy polyunsaturated fatty acid profile. Abbreviations FAFatty acids AANAT Aralkylamine N-acetyltransferase ADRB3 Adrenergic beta-3 receptor BTG2 B-cell translocation gene 2 FASN Fatty acid synthase

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Section: Discussionmentioning

confidence: 96%

Molecular genetics-nutrition interactions in the expression of AANAT, ADRB3, BTG2 and FASN genes in the heart, kidney and liver of Australian lambs supplemented with Spirulina (Arthrospira platensis)

Malau‐Aduli

Kashani

2015

Genes Genom

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“…The global nature of genomics technology could be then an advantage for elucidating the complex physiological control of IMF fat content, which is likely mediated through multiple biochemical and molecular mechanisms in both adipocytes and myofibres. Differential-display reverse transcription polymerase chain reaction (ddRT-PCR; Sasaki et al, 2006;Lee et al, 2007), microarrays (Wang et al, 2005aand 2005b, Kolditz et al, 2008Liu et al, 2009) eventually using musclededicated (Sudre et al, 2005) or fat-enriched arrays (Lehnert et al, 2006) and proteomics (Kolditz et al, 2008;Liu et al, 2009) have been recently used in muscles of livestock animals and fishes to examine gene and protein expressions at different developmental stages during fattening period, between two breeds or two selected lines with a different propensity to accumulate IMF fat, and (or) between individuals with contrasting levels for this trait. In some studies, it cannot be excluded that the differences seen in gene expressions are due to breed characteristics rather than to variations in IMF per se.…”

Section: Genetic Markersmentioning

confidence: 99%

Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers

Hocquette

Gondret

Baéza³

et al. 2010

Animal

614

447

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Intramuscular fat (IMF) content plays a key role in various quality traits of meat. IMF content varies between species, between breeds and between muscle types in the same breed. Other factors are involved in the variation of IMF content in animals, including gender, age and feeding. Variability in IMF content is mainly linked to the number and size of intramuscular adipocytes. The accretion rate of IMF depends on the muscle growth rate. For instance, animals having a high muscularity with a high glycolytic activity display a reduced development of IMF. This suggests that muscle cells and adipocytes interplay during growth. In addition, early events that influence adipogenesis inside the muscle (i.e proliferation and differentiation of adipose cells, the connective structure embedding adipocytes) might be involved in interindividual differences in IMF content. Increasing muscularity will also dilute the final fat content of muscle. At the metabolic level, IMF content results from the balance between uptake, synthesis and degradation of triacylglycerols, which involve many metabolic pathways in both adipocytes and myofibres. Various experiments revealed an association between IMF level and the muscle content in adipocyte-type fatty acid-binding protein, the activities of oxidative enzymes, or the delta-6-desaturase level; however, other studies failed to confirm such relationships. This might be due to the importance of fatty acid fluxes that is likely to be responsible for variability in IMF content during the postnatal period rather than the control of one single pathway. This is evident in the muscle of most fish species in which triacylglycerol synthesis is almost zero. Genetic approaches for increasing IMF have been focused on live animal ultrasound to derive estimated breeding values. More recently, efforts have concentrated on discovering DNA markers that change the distribution of fat in the body (i.e. towards IMF at the expense of the carcass fatness). Thanks to the exhaustive nature of genomics (transcriptomics and proteomics), our knowledge on fat accumulation in muscles is now being underpinned. Metabolic specificities of intramuscular adipocytes have also been demonstrated, as compared to other depots. Nutritional manipulation of IMF independently from body fat depots has proved to be more difficult to achieve than genetic strategies to have lipid deposition dependent of adipose tissue location. In addition, the biological mechanisms that explain the variability of IMF content differ between genetic and nutritional factors. The nutritional regulation of IMF also differs between ruminants, monogastrics and fish due to their digestive and nutritional particularities.

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“…This syndrome in humans is caused by heterozygous deletions of about 1.6 Mb, and one of the numerous symp- (Sasaki et al, 2006;Serao et al, 2011) 18 56 485 824-57 128 560 R PSMA2 negative correlation with male BMI (Sakamoto et al, 2009) toms in about 15 % of the cases is hypercalcemia (Cagle et al, 2004). Thus, at least one of the genes in this region must have an influence on blood calcium levels.…”

Section: Resultsmentioning

confidence: 99%

“…The decision about a possible candidate status was thus hindered. However, in 7 of the 10 remaining regions, possible candidate genes could be identified: WBSCR17 (International Mouse Phenotyping Consortium), SRSF7 (International Mouse Phenotyping Consortium), USP28 (Valero et al, 2001), NNMT (Trammell and Brenner, 2015), ZBED6 (Clark et al, 2015;Markljung et al, 2009), NMNAT2 (Hicks et al, 2012), ABHD6 (Thomas et al, 2013), PDHB (Sasaki et al, 2006;Serao et al, 2011) and PSMA2 (Sakamoto et al, 2009). These are listed in Table 2.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide QTL mapping results for regional DXA body composition and bone mineral density traits in pigs

Bernau¹,

Kremer-Rücker

Međugorac³

et al. 2017

Arch. Anim. Breed.

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Abstract. In a previous study, genome-wide mapping of quantitative trait loci (QTL) for five body composition traits, three bone mineral traits and live weight was performed using whole-body dual-energy X-ray absorptiometry (DXA) data. Since QTL for bone mineral traits were rare, the current study aimed to clarify whether the mapping results were influenced by the analysed body regions. Thus, the same material (551 pigs) and methods as in the whole-body QTL mapping study were used. However, for evaluation of the DXA scans, we manually defined two body regions: (i) from the last ribs to the pelvis (A) and (ii) including the pelvis and the hind limbs (P). Since live weight was not affected by the regional analysis, it was omitted from the QTL mapping design.Our results show an overall high consistency of mapping results especially for body composition traits. Two thirds of the initial whole-body QTL are significant for both A and P. Possible causes for the still low number of bone mineral QTL and the lower consistency found for these traits are discussed. For body composition traits, the data presented here show high genome-wide Pearson correlations between mapping results that are based on DXA scans with the time-saving "whole-body standard setting" and mapping results for DXA data that were obtained by time-consuming manual definition of the regions of interest. However, our results also suggest that whole-body or regional DXA scans might generally be less suitable for mapping of bone mineral traits in pigs. An analysis of single reference bones could be more useful.

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Exploration of genes showing intramuscular fat deposition‐associated expression changes in musculus longissimus muscle

Cited by 87 publications

References 16 publications

Molecular genetics-nutrition interactions in the expression of AANAT, ADRB3, BTG2 and FASN genes in the heart, kidney and liver of Australian lambs supplemented with Spirulina (Arthrospira platensis)

Molecular genetics-nutrition interactions in the expression of AANAT, ADRB3, BTG2 and FASN genes in the heart, kidney and liver of Australian lambs supplemented with Spirulina (Arthrospira platensis)

Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers

Genome-wide QTL mapping results for regional DXA body composition and bone mineral density traits in pigs

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