Exploration of Fluorescence Quenching Mechanism in Tryptophan Induced by Norfloxacin: Analytical Applications

Abstract

Fluorescence quenching mechanism of tryptophan (Trp) by norfloxacin (NORF) was investigated by spectrofluorometric method at physiological pH. Stern−Volmer quenching constant is positively related with temperature shows the dynamic type of quenching. Life time of Trp decreases by the addition of NORF supports dynamic type of quenching. Fluorescence quenching method was successfully applied for direct determination of NORF from pharmaceutical sample.