Exploration of Floral Volatile Organic Compounds in Six Typical Lycoris taxa by GC-MS

Shi, Tingting; Yue, Yuanzheng; Shi, Man; Chen, Min; Yang, Xia; Wang, Lianggui

doi:10.3390/plants8100422

Cited by 14 publications

(10 citation statements)

References 53 publications

(59 reference statements)

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“…Lycoris sprengeri is an important herb, and the Lycoris genus has about 20 species, of which ten are commonly grown in China and Southern Asia ( Shi et al, 2019 ). Previous transcriptomic studies relevant to the Amaryllidaceae family investigated L. aurea ( Wang et al, 2013 ), L. longituba ( Wang et al, 2013 ; Li et al, 2020 ), Allium cepa ( Kim et al, 2015 ), Narcissus pseudonarcissus ( Singh and Desgagné-Penix, 2017 ), and L. sprengeri ( Chang et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri

Feng

Das

et al. 2021

Front. Plant Sci.

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Lycoris sprengeri (L. sprengeri) is an important ornamental bulbous plant, and its numerous varieties in different color forms are widely planted. Multiple color types of petals in L. sprengeri provide us with possibilities to delineate the complicated metabolic networks underlying the biochemical traits behind color formation in this plant species, especially petal color. In this study, we sequenced and annotated a reference transcriptome of pink and white petals of L. sprengeri and analyzed the metabolic role of anthocyanin biosynthesis in regulating color pigment metabolism. Briefly, white and pink petal samples were sequenced with an Illumina platform, to obtain the reads that could be assembled into 100,778 unique sequences. Sequences expressed differentially between white vs. pink petals were further annotated with the terms of Gene Ontology (GO), Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG), and eggNOG. Gene expression analyses revealed the repression of anthocyanin and steroid biosynthesis enzymes and R2R3 MYB transcription factor (TF) genes in white petals compared to pink petals. Furthermore, the targeted metabolic profiling of anthocyanins revealed that color-related delphinidin (Del) and cyanidin (Cy) pigments are lower in white petals, which correlate well with the reduced gene expression levels of anthocyanin biosynthesis genes. Taken together, it is hypothesized that anthocyanin biosynthesis, steroid biosynthesis, and R2R3 MYB TFs may play vital regulatory roles in petal color development in L. sprengeri. This work provides a valuable genomic resource for flower breeding and metabolic engineering in horticulture and markers for studying the flower trait evolution of L. sprengeri.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri

Feng

Das

et al. 2021

Front. Plant Sci.

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“…However, the emission of a-farnesene together with other potent scents compounds (linalool and phenylethyl salicylate ) increased the numbers of pollinators such as bees and flies which acting like a behavioral modifier to filter out certain species of pollinators. Solid Phase Micro Extraction (SPME) was a method that was widely used to trap and capture emitted volatiles for analysis (Shi et al 2019). Compared to standard headspace extraction that was conducted previously by Hsiao et al (2006) and Chuang et al, (2017), this method managed to profile a broader range of volatiles, in which several new compounds were managed to identify such as copaene, bergamiol, and linalyl anthranilate.…”

Section: Effect Of Different Observation Period On the Phalaenopsis Bellina Floral Scent Emissionmentioning

confidence: 99%

November 2020

2020

Plant Omics

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Phalaenopsis bellina, is an orchid widely known for their distinctive fragrance. Of late, fragrant orchids are getting more attention from Orchid breeders for their horticultural market value. Although volatile compounds have been identified in several orchid species, the floral scent emission of P. bellina is far from understood. Therefore, this study was carried out to investigate the influence of different timing and floral development stages on the volatile emissions from P. bellina flowers using Solid Phase Micro Extraction (SPME) and Gas Chromatography-Mass Spectrometry (GC-MS). The volatile compound was extracted during morning and afternoon sessions from three different floral developmental stages. The volatile compounds emitted from flowers from two different sessions were trapped with SPME fiber for 30 minutes before directly injected into GC-MS for identification. Results showed that approximately 79 volatile compounds were identified, with the terpenoid presented as the major compound class. P. bellina had the highest number of volatiles during the morning and full bloom (41), with 29.82% monoterpene and 23.33% sesquiterpenes accounted in total. α-farnesene (19.56%) was abundance during morning emission and remains as the highest volatile in afternoon emission (44.08%), even higher when compared to morning emission. However, a decrease in the volatile compound was observed in afternoon emission, in which only 34 volatile compounds detected. Meanwhile, partial bloom developmental stage revealed linalool as the major terpenoid volatile compounds (25.89%), with only 20 volatile compounds recorded. On the other hand, no volatile compounds were recorded and profiled from flower bud stage. The establishment of a floral scent study provides a brief overview of the regulation of fragrance in P. bellina, which can be continued through gene isolation or fragrance-related enzymes study. This information will provide necessary information on orchid floral scent research that useful in boosting horticultural trade of the scented orchids and their function in pollination ecology study.

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“…Concerning the non-fragrant plants, some species can be scentless for human beings while other species might have a subtle scent only detected by some people. Non-fragrant flowers also emit different VOCs such as aliphatic aldehydes, aliphatic alcohols, and phenylpropanoids/benzenoids [ 13 , 14 ]. VOCs’ role in plants is essential and varied from pollinator attraction to defense against predators and growth [ 4 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Extraction and Identification of Volatile Organic Compounds in Scentless Flowers of 14 Tillandsia Species Using HS-SPME/GC-MS

et al. 2022

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VOCs emitted by flowers play an important role in plant ecology. In the past few years, the Tillandsia genus has been scarcely studied according to the VOCs emitted by flowers. Hence, we decided to enlarge the VOCs composition study already undergone in our laboratory on fragrant 3 Tillandsia species to 12 unscented and 2 faint-scented Tillandsia species and hybrids. The headspace solid phase microextraction (HS-SPME) coupled with gas chromatography combined with the mass spectrometry (GC-MS) method was used to explore the chemical diversity of the VOCs. This study allowed the identification of 65 VOCs among the 14 species and between 6 to 25 compounds were identified in each of the species. The aromatic profile of 10 of the species and hybrids are similar to each other’s and show 8 predominant compounds: benzaldehyde, benzacetaldehyde, hexanol, hexanal, heptanal, octanal, nonanal, and furan-2-pentyl. Some specific compounds are present only in some unique species such as trans-calamenene, α-muurolene, and α-guaiene trans-β-bergamotene. The two faint-scented species studied present an original aromatic profile with a high number of monoterpenes or phenylpropanoids/benzenoids. Our studies allow a better understanding of the ecological role and function of these VOCs in the interactions between these plants with their environment.

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Exploration of Floral Volatile Organic Compounds in Six Typical Lycoris taxa by GC-MS

Cited by 14 publications

References 53 publications

Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri

Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri

November 2020

Extraction and Identification of Volatile Organic Compounds in Scentless Flowers of 14 Tillandsia Species Using HS-SPME/GC-MS

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