Exploration into Natural Variation Genes Associated with Determinate and Capitulum-like Inflorescence in Brassica napus

Abstract

Brassica napus is a globally important vegetable and oil crop. The research is meaningful for the yield and plant architecture of B. napus. In this study, one natural mutant line with determinate and capitulum-like inflorescence was chosen for further study. Genetic analysis indicated that the segregation patterns of inflorescences in the F2 populations supported a digenic inheritance model, which was further approved via the BSA-Seq technique. The BSA-Seq method detected two QTL regions on C02 (14.27–18.41 Mb) and C06 (32.98–33.68 Mb) for the genetic control of determinate inflorescences in MT plants. In addition, the expression profile in MT compared with WT was analyzed, and a total of 133 candidate genes for regulating the flower development (75 genes, 56.4%), shoot meristem development (29 genes, 21.8%), and inflorescence meristem development (13 genes, 9.8%) were identified. Then one joint analysis combing BSA-Seq and RNA-Seq identified two candidate genes of BnaTFL1 and BnaAP1 for regulating the MT phenotype. Furthermore, the potential utilization of the MT plants was also discussed.

Lipid and sugar metabolism play an essential role in pollen development and male sterility: a case analysis in Brassica napus

“…Sixteen gene‐specific primers for qRT‐PCR were designed based on reference unigene sequences randomly chosen from the DEGs using Primer Premier 5.0. Real‐time PCR was conducted using SsoAdvancedTM Universal SYBRR Green Supermix according to our earlier research (Khattak et al 2019, Wan et al 2023). The 2 −ΔΔCt algorithm was used to calculate the relative level of gene expression.…”

Fine Mapping and Functional Verification of the Brdt1 Gene Controlling Determinate Inflorescence in Brassica rapa L.

“…Further investigation revealed that this trait was controlled by two independently inherited recessive genes (Bnsdt1 and Bnsdt2). Wan et al [15] employed BSA-Seq technology to identify two QTL loci associated with determinate inflorescence in a mutant of B. napus. These loci were found on the C02 and C06 chromosomes, respectively.…”