Exploiting next generation sequencing techniques (NGS) to identify molecular markers for monitoring the resistance of &lt;i&gt;Spodoptera frugiperda&lt;/i&gt; (J.E. Smith) (Lepidoptera: Noctuidae) to insecticides and Bt proteins

Nascimento, Antônio Rogério Bezerra do; Omoto, Celso

doi:10.11606/t.11.2019.tde-03012019-160052

Cited by 3 publications

(3 citation statements)

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“…When evaluating the response of heterozygotes exposed to spinetoram, the results indicate that the genes related to S. frugiperda resistance to spinetoram are located in autosomal chromosomes, and therefore shared by males and females. Autosomal inheritance is commonly associated with resistance of fall armyworm to insecticides, including spinosad . Our results about D suggest incompletely recessive spinetoram resistance in S. frugiperda .…”

Section: Discussionmentioning

confidence: 58%

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Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to spinetoram: inheritance and cross‐resistance to spinosad

Lira

Bolzan

Nascimento

et al. 2020

Pest Management Science

107

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BACKGROUNDThe use of spinosyn insecticides is one of the major control strategies of the fall armyworm, Spodoptera frugiperda (J. E. Smith) in Brazil. In this study, we selected a spinetoram‐resistant strain from a field‐population of S. frugiperda to characterize the inheritance of the resistance and cross‐resistance relationship between spinosyn insecticides.RESULTSThe values of LC50 (95% CI) obtained from concentration–response bioassays were 0.63 (0.55–0.73) μg spinetoram mL−1 for the susceptible strain (SUS), and 1170.96 (1041.61–1323.89) μg spinetoram mL−1 for the strain resistant to spinetoram (SPT‐R). These values resulted in a resistance ratio of 1844‐fold. The SPT‐R strain showed cross‐resistance with spinosad (resistance ratio = 1196‐fold). The reciprocal crosses showed LC50 values of 3.91 (2.97–5.84) and 5.37 (4.52–6.52) μg spinetoram mL−1, suggesting that the resistance of S. frugiperda to spinetoram is autosomal and incompletely recessive. The backcrosses of the F1 progeny with the SPT‐R strain suggest a resistance with polygenic effect. Estimates of the effective number of loci with equal contributions to the resistance effect were from 1.18 to 1.76, suggesting that resistance to spinetoram is associated with a few genes.CONCLUSIONThe inheritance pattern of resistance of S. frugiperda to spinetoram was characterized as autosomal, incompletely recessive and polygenic. Cross‐resistance between spinosyns was confirmed in S. frugiperda. The importance of this information for implementing insect resistance management strategies is discussed in this paper. © 2020 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 58%

“…Autosomal inheritance is commonly associated with resistance of fall armyworm to insecticides, including spinosad. 19,[36][37][38][39][40][41][42] Our results about D suggest incompletely recessive spinetoram resistance in S. frugiperda. However, dominance levels varied depending upon the concentration.…”

Section: Discussionmentioning

confidence: 99%

Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to spinetoram: inheritance and cross‐resistance to spinosad

Lira

Bolzan

Nascimento

et al. 2020

Pest Management Science

107

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“…De acordo com Arthropod Pesticide Resistance Database, há registros de 144 casos de resistência de S. frugiperda a inseticidas (APRD 2021). No Brasil, casos de resistência de S. frugiperda para diferentes grupos de inseticidas, tais como piretroides, organofosforados, inibidores de biossíntese de quitina, espinosinas e diamidas já foram documentados , Bolzan et al 2019, Nascimento 2019, assim como para as proteínas Cry1F ) e Cry1Ab ) expressas em híbridos de milho geneticamente modificado que expressam toxinas de Bacillus thuringiensis Berliner (Bt), comprometendo outras tecnologias Bt devido à resistência cruzada entre proteínas do grupo Cry1 . Sendo assim, o uso de inseticidas para o manejo de S. frugiperda ainda tem sido necessário no Brasil.…”

Section: Introductionunclassified

Resistência de >i<Spodoptera frugiperda>/i< (Lepidoptera: Noctuidae) a inseticidas bloqueadores de canais de sódio

Kaiser¹

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Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to sodium channel blocker insecticides Spodoptera frugiperda (Lepidoptera: Noctuidae) is the major maize pest in Brazil, and its control have been based on the use insecticides and transgenic plants expressing proteins of Bacillus thuringiensis Berliner (Bt). Several cases of S. frugiperda resistance to these control tactics have already been documented and resistance has been one of the major threats to integrated pest management (IPM) programs. Therefore, the use of insecticides with new modes of action such as sodium channel blockers (indoxacarb and metaflumizone) could help in insect resistance management programs (IRM). To develop an IRM program, the goals of this research were (i) To characterize the baseline susceptibility to indoxacarb and metaflumizone in S. frugiperda populations colletected from major maize-growing regions in Brazil; (ii) To monitor the S. frugiperda resistance to indoxacarb and metaflimizone in populations collected from 2017 to 2020 growing seasons; (iii) To evaluate the response to indoxacarb and metaflumizone in strains of S. frugiperda susceptible and resistant to some selected insecticides and Bt proteins; (iv) To select a resitant strain of S. frugiperda resistant to sodium channel blockers insecticides under laboratory coditions to characterize the genetic basis of resistance and cross-resistance between insecticides that act on sodium channels; and (v) To evaluate fitness costs associated with resistance of S. frugiperda to indoxacarb and metaflumizone. Low variation in susceptibility to indoxacarb (4.6-fold) and metaflumizone (2.6-fold) was detected in populations of S. frugiperda collected in 2017. The frequency of the resistance allele to indoxacarb was 0.0452 (0.0382 -0.0527 CI 95%), by using F2 screen method. The mean survival at diagnostic concentration, based on LC99, varied from 0.2 to 12.2% for indoxacarb and from 0.0 to 12.7% for metaflumizone in populations of S. frugiperda collected in Brazil from 2017 to 2020. The resistance ratio of S. frugiperda to indoxacarb was ≈ 30-fold and to metaflumizone was > 600-fold. No cross-resistance was detected between sodium channel blocker insecticides (indoxacarb and metaflumizone) and other insecticides (chlorpyrifos, lambda-cyhalothrin, lufenuron, teflubenzuron, spinetoram and chlorantraniliprole) and Bt proteins (Cry1A.105/Cry2Ab2/Cry1F and Vip3Aa20). On the other hand, cross-resistance between indoxacarb and metaflumizone was detected. The inheritance of resistance of S. frugiperda to indoxacarb and metaflumizone was autosomal, incompletely recessive and with polygenic effect. In addition, significant fitness costs associated with S. frugiperda resistance to indoxacarb and metaflumizone were observed in life history traits evaluated under laboratory conditions in the absence of selection pressure. Thus, based on information obtained in this study, indoxacarb and metaflumizone are important tools to be exploited in IRM programs in Brazil.

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Baseline Response, Monitoring, and Cross-Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to Sodium Channel Blocker Insecticides in Brazil

Kaiser

Kanno

Bolzan

et al. 2021

Journal of Economic Entomology

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Spodoptera frugiperda (J.E. Smith) is one of the key cross-crop pests in Brazilian agroecosystems. Field-evolved resistance of S. frugiperda to some conventional insecticides and Bt proteins has already been reported. Thus, the use of insecticides with new mode of action such as sodium channel blockers (indoxacarb and metaflumizone) could be an important tool in insecticide resistance management (IRM) programs. To implement a proactive IRM, we conducted baseline response and monitoring to indoxacarb and metaflumizone in 87 field populations of S. frugiperda collected from major maize-growing regions of Brazil from 2017 to 2020, estimated the frequency of resistance alleles to indoxacarb, and evaluated cross-resistance of indoxacarb and metaflumizone to some selected insecticides and Bt proteins. Low variation in susceptibility to indoxacarb (4.6-fold) and metaflumizone (2.6-fold) was detected in populations of S. frugiperda in 2017. The frequency of the resistance allele to indoxacarb was 0.0452 (0.0382–0.0527 CI 95%), by using F2 screen method. The mean survival at diagnostic concentration, based on CL99, varied from 0.2 to 12.2% for indoxacarb and from 0.0 to 12.7% for metaflumizone, confirming high susceptibility of S. frugiperda to these insecticides in Brazil. No cross-resistance was detected between sodium channel blocker insecticides and other insecticides (organophosphate, pyrethroid, benzoylurea, spinosyn, and diamide) and Bt proteins. These findings showed that sodium channel blocker insecticides are important candidates to be exploited in IRM strategies of S. frugiperda in Brazil.

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Exploiting next generation sequencing techniques (NGS) to identify molecular markers for monitoring the resistance of <i>Spodoptera frugiperda</i> (J.E. Smith) (Lepidoptera: Noctuidae) to insecticides and Bt proteins

Cited by 3 publications

References 45 publications

Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to spinetoram: inheritance and cross‐resistance to spinosad

Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to spinetoram: inheritance and cross‐resistance to spinosad

Resistência de >i<Spodoptera frugiperda>/i< (Lepidoptera: Noctuidae) a inseticidas bloqueadores de canais de sódio

Baseline Response, Monitoring, and Cross-Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to Sodium Channel Blocker Insecticides in Brazil

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