Exploiting genomic data to identify proteins involved in abalone reproduction

Identification of differentially expressed reproductive and metabolic proteins in the female abalone ( Haliotis laevigata ) gonad following artificial induction of spawning

“…6a). The MRM-MS analysis found that Vg was the most abundant of the targeted proteins in female abalone gonad in agreement with our previous finding where analysis of spectral redundancy and precursor signal intensity deemed Vg as the most abundant protein in gonads of female H. laevigata (Mendoza-Porras et al, 2014).…”

“…A subset of these proteins was then quantitatively assessed across 18 individual abalone gonads wherein four were from PSP abalone, five from SP and nine from FSP abalone. Aside from key proteins identified in the 2D-DIGE approach, this analysis was extended to include other proteins identified reported in a previous study (Mendoza-Porras et al, 2014) such as vitelline envelope receptor for lysin (VERL) (results in Supp. File 2) and prohibitin (PHB) that are known to participate in reproductive processes in abalone and closelyrelated molluscs.…”

Alternative Splicing Profile and Sex-Preferential Gene Expression in the Female and Male Pacific Abalone Haliotis discus hannai

“…In order to test sex-preferential expression from each transcriptome, quantitative real-time RT-PCR (qPCR) was performed using six sex-preferential genes: vitellogenin ( VTG ), forkhead box protein L2 ( FOXL2 ), condensin-2 , sperm-associated antigen 6 ( SPAG6 ), protein fem-1 homolog C-like ( FEM1-like ), and tektin-1 , which are relatively well-characterized in marine invertebrates [9,21,22,23,24]. Based on the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines [25], transcriptional levels of each sex-preferential gene were validated using qPCR.…”

“…Genes involved in numerous metabolic pathways such as innate immunity, cell stress and repair system, and antioxidant defense systems have been cloned and tested to determine their mRNA expression, translational inducibility, and molecular functions. Recently, the use of a genomics platform was successfully applied in H. discus hannai , although many research groups have continuously employed ‘-omics’ platforms (e.g., genomics, proteomics, linkage map development, Bacterial Artificial Chromosome (BAC) library construction) to understand the growth, development, reproduction, molecular adaptation, or innate immune response mechanisms upon environmental stressors or pathogen challenges in the Haliotis genus [5,6,7,8,9,10,11,12,13,14]. For H. discus hannai , a genetic linkage map was constructed as a potential application of marker-assisted selection in breeding programs using amplified fragment length polymorphism (AFLP) markers [15].…”

Identification of a female spawn‐associated Kazal‐type inhibitor from the tropical abalone Haliotis asinina

“…A comprehensive mass spectrometry‐based proteome analysis has been established for the abalone Haliotis laevigata gonad, showing 162 and 110 proteins in female and male gonads, respectively . The method used for purification (2D gel electrophoresis) did remove biomolecules less than >10 kDa, and therefore a peptide similar to that identified in this study was not found.…”