Exploitation of Rhizosphere Microbiome Services

Riva, Valentina; Terzaghi, Elisa; Vergani, Lorenzo; Mapelli, Francesca; Zanardini, Elisabetta; Morosini, Cristiana; Raspa, G.; Guardo, Antonio Di; Borin, Sara

doi:10.1007/978-981-13-5767-1_7

Cited by 11 publications

(7 citation statements)

References 94 publications

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“…The PGP potential of the selected isolates was initially analyzed in this study through in vitro tests. The two Paenarthrobacter strains showed potential biostimulant capacity, producing auxin or degrading ACC: under drought stress conditions, these two activities can improve plant growth by promoting root system development and expansion for the absorption of the greatest amount of water and by reducing the plant stress perception ( Riva et al, 2019b ). Besides biostimulant abilities, M. yunnanensis M1, B. simplex RP-26, and P. stutzeri SR7-77 also showed the potential to improve the nutrient uptake through ammonia and siderophore production or phosphate solubilization.…”

Section: Discussionmentioning

confidence: 99%

“…In adverse environmental conditions, in particular, plants have evolved with a complex microbial community enriched in PGP microorganisms able to counteract abiotic stress and to confer a certain level of stress tolerance to the plant host ( Soussi et al, 2016 ; Riva et al, 2019b ; Sangiorgio et al, 2020 ). Plants adapted to extreme environmental conditions, thus, represent an interesting source of PGP microorganisms, which could potentially boost the growth of other plant species, including agricultural crops, improving their ability to cope with abiotic stress ( Yuan et al, 2016 ; Chen et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

et al. 2021

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Global population growth and climate change raise a challenge to agriculture, which, combined with the issues concerning the use of chemical fertilizers, have generated increasing attention in the use of plant-associated bacteria as a sustainable strategy in agri-food systems. The objective of this study is to evaluate the ability of five bacterial strains, previously isolated from the rhizosphere or endosphere of plants adapted to harsh environmental conditions, to act as potential plant biofertilizers in different conditions of water availability. The strain biosafety for a deliberate environmental release was investigated through a literature survey and antibiotic resistance testing. The selected strains were first characterized for their plant growth–promoting (PGP) and rhizocompetence-related traits through in vitro assays and then on short-term in vivo experiments on tomato plants. A long-term greenhouse experiment was further conducted to monitor the PGP effect of the bacteria during the entire life cycle of tomato plants subjected to full irrigation or to severe water deficit conditions, aiming to assess their actual effect on plant productivity, which is the ultimate target of the agricultural sector. Some of the strains showed a potential in improving water use efficiency and mitigating plant water stress. Under severe irrigation deficit, four of the tested strains, Micrococcus yunnanensis M1, Bacillus simplex RP-26, Pseudomonas stutzeri SR7-77, and Paenarthrobacter nitroguajacolicus 2–50, significantly increased the number of productive plants in comparison to non-bacterized control ones. Two of them, Bacillus simplex RP-26 and Paenarthrobacter nitroguajacolicus 2–50, demonstrated also, under full irrigation, to significantly improve the water productivity in comparison with non-bacterized plants. Despite all the strains showed promising PGP potential in short-term assays, the positive effect of the bacterial inoculants on plant physiology and fruit yield was observed in some cases but never corroborated by statistical significance. These results highlight the importance of performing long-term in vivo experiments to define the real PGP ability of a bacterial inoculant to positively impact plant production.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

et al. 2021

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“…There are many evidences that plant-associated bacteria are able to sustain plant growth under adverse environmental conditions [13,73]; however, no report exists in the literature on the identity and the PGP potential of the argan tree-associated bacteria. We established a collection of 371 bacterial isolates from the root system (E-R-SSR fractions) of A. spinosa, its residuesphere and the bulk soil, using the same cultivation medium for all the fractions in order to compare their composition in terms of cultivable bacteria taxonomy.…”

Section: Phylogenetic Classification and Distribution Of The Cultivabmentioning

confidence: 99%

Unveiling the Microbiota Diversity of the Xerophyte Argania spinosa L. Skeels Root System and Residuesphere

et al. 2020

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The microbiota associated to xerophyte is a “black box” that might include microbes involved in plant adaptation to the extreme conditions that characterize their habitat, like water shortage. In this work, we studied the bacterial communities inhabiting the root system of Argania spinosa L. Skeels, a tree of high economic value and ecological relevance in Northern Africa. Illumina 16S rRNA gene sequencing and cultivation techniques were applied to unravel the bacterial microbiota’s structure in environmental niches associated to argan plants (i.e., root endosphere, rhizosphere, root-surrounding soil), not associated to the plant (i.e., bulk soil), and indirectly influenced by the plant being partially composed by its leafy residue and the associated microbes (i.e., residuesphere). Illumina dataset indicated that the root system portions of A. spinosa hosted different bacterial communities according to their degree of association with the plant, enriching for taxa typical of the plant microbiome. Similar alpha- and beta-diversity trends were observed for the total microbiota and its cultivable fraction, which included 371 isolates. In particular, the residuesphere was the niche with the highest bacterial diversity. The Plant Growth Promotion (PGP) potential of 219 isolates was investigated in vitro, assessing several traits related to biofertilization and biocontrol, besides the production of exopolysaccharides. Most of the multivalent isolates showing the higher PGP score were identified in the residuesphere, suggesting it as a habitat that favor their proliferation. We hypothesized that these bacteria can contribute, in partnership with the argan root system, to the litter effect played by this tree in its native arid lands.

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“…Paenarthrobacter strains have a potential biostimulatory capacity via the production of auxin or decomposition of amino acids. Under drought stress, they can improve plant growth through the promotion of the development and expansion of the root system in order to uptake the greatest amount of water and reduce stress perception in plants [ 87 ].…”

Section: Resultsmentioning

confidence: 99%

Impact of Soil Fertilized with Biomass Ash on Depth-Related Variability of Culturable Bacterial Diversity and Selected Physicochemical Parameters in Spring Barley Cultivation

Pastuszczak

Stanek-Tarkowska

Kačániová

2022

IJERPH

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This study investigated the effect of different doses of fertilization with biomass combustion ash (Salix viminalis L. willow) on changes in the biological, chemical, and physical properties of soil. The experiment was carried out on podzolic and chernozem soils in a one-way field experiment (fertilization dose: control (without fertilization), NPK (nitrogen (N), phosphorus (P) and potassium (K)), 100, 200, 300, 400, 500 kg K2O ha−1). The biomass ash was characterized by a pH value of 12.83 ± 0.68 and a high content of macronutrients. The samples were collected from 0–5, 10–15, and 20–25 cm soil layers under the cultivation of spring barley (Hordeum vulgare L) cv. Planet in April and August 2021. Mass spectrometry (MALDI-TOF MS) was used for microbiological analyses, which revealed the presence of 53 culturable species from 11 genera: Bacillus, Pseudomonas, Paenibacillus, Lysinibacillus, Pseudarthrobacter, Arthrobacter, Staphylococcus, Paenarthrobacter, Micrococcus, Rhodococcus, and Flavobacterium. The podzolic and chernozem soils exhibited the presence of 28 and 44 culturable species, respectively. The study showed an increase in the number of microorganisms in the top layer of the soil profile. However, the number of bacteria decreased at the depths of 10–15 cm and 20–25 cm. With depth, the bulk density (BD) and moisture increased.

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Exploitation of Rhizosphere Microbiome Services

Cited by 11 publications

References 94 publications

Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

Bacterial Inoculants Mitigating Water Scarcity in Tomato: The Importance of Long-Term in vivo Experiments

Unveiling the Microbiota Diversity of the Xerophyte Argania spinosa L. Skeels Root System and Residuesphere

Impact of Soil Fertilized with Biomass Ash on Depth-Related Variability of Culturable Bacterial Diversity and Selected Physicochemical Parameters in Spring Barley Cultivation

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