Exploitation of reverse vaccinology and immunoinformatics as promising platform for genome-wide screening of new effective vaccine candidates against Plasmodium falciparum

Pritam, Manisha; Singh, Garima; Swaroop, Suchit; Singh, Akhilesh Kumar; Singh, Satarudra Prakash

doi:10.1186/s12859-018-2482-x

Cited by 31 publications

(20 citation statements)

References 65 publications

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“…These PVCs are poise to induce both B-cell and T-cells responses. Reverse vaccinology approaches have been applied in several infectious diseases such as malaria (Tuju et al 2017), multiple sclerosis (Islam et.al 2020) and Plasmodium falciparum (Pritam et al 2019). Even though there have been several endevours to develop vaccines pertaining to specific diseases, the attempts were unfruitful.…”

Section: Discussionmentioning

confidence: 99%

Designing of multiepitope-based vaccine against Leptospirosis using Immuno-Informatics approaches

Abdullah

Kadivella

Sharma

et al. 2021

Preprint

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Leptospira is a zoonotic pathogen causing significant morbidity and mortality both in animals and humans. Although several surface proteins have been identified as vaccine candidate, they failed to induce sterilizing immunity and cross protection against different serovars. Thus, identification of highly immunogenic antigens that are conserved among pathogenic serovars would be first step towards development of universal vaccine for Leptospirosis. Here we used reverse vaccinology pipeline to screen core genome of pathogenic Leptospira spp.in order to identify suitable vaccine candidates. Based on properties like sub cellular localization, adhesin, homology to human proteins, antigenicity and allergenicity, 18 antigenic proteins were identified through this pipeline and were further investigated for immunological properties. Based on immunogenicity, Protegenicity, Antigenicity, B-cell and promiscuous T-cell epitopes, 6 Potential Vaccine Candidates (PVCs) were finally selected which covered most of the affected world population. For designing a Multi-Epitope Vaccine (MEV), 6 B-cell and 6 promiscuous MHC-I and MHC-II epitopes from each candidate were clustered with linkers in between and stitched along with a TLR4 adjuvant (APPHALS) at the N-terminal forming a construct of 361 amino acids. The physiochemical properties, secondary and tertiary structure analysis revealed that MEV was highly stable. Molecular docking analysis revealed the deep binding interactions of the MEV construct within the grooves of human TLR4 (4G8A). In-silico codon optimization and cloning of the vaccine construct assured good expression. Further, immune simulations have shown that MEV could induce strong and diverse B-cell and T-cell responses. Taken together our results indicate that the designed MEV could be a promising subunit vaccine candidate against Leptospirosis, however it requires experimental validation.

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Section: Discussionmentioning

confidence: 99%

Designing of multiepitope-based vaccine against Leptospirosis using Immuno-Informatics approaches

Abdullah

Kadivella

Sharma

et al. 2021

Preprint

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“…To handle evidence diversity, immunoinformatics uses tools that cut across several aspects of bioinformatics such as creation and management of databases, 34,35 use and definition of both structural and functional signatures and the formation and application of predictive tools. [35][36][37] These strategies can synergize toward a better understanding of the immune system of both man and animals and fight against some less predictable pathogenesis.…”

Section: Immunoinformatics and Infectious Diseasementioning

confidence: 99%

<p>Immunoinformatics and Vaccine Development: An Overview</p>

Oli

Obialor

Ifeanyichukwu

et al. 2020

ITT

158

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The use of vaccines have resulted in a remarkable improvement in global health. It has saved several lives, reduced treatment costs and raised the quality of animal and human lives. Current traditional vaccines came empirically with either vague or completely no knowledge of how they modulate our immune system. Even at the face of potential vaccine design advance, immune-related concerns (as seen with specific vulnerable populations, cases of emerging/reemerging infectious disease, pathogens with complex lifecycle and antigenic variability, need for personalized vaccinations, and concerns for vaccines' immunological safety -specifically vaccine likelihood to trigger non-antigen-specific responses that may cause autoimmunity and vaccine allergy) are being raised. And these concerns have driven immunologists toward research for a better approach to vaccine design that will consider these challenges. Currently, immunoinformatics has paved the way for a better understanding of some infectious disease pathogenesis, diagnosis, immune system response and computational vaccinology. The importance of this immunoinformatics in the study of infectious diseases is diverse in terms of computational approaches used, but is united by common qualities related to host-pathogen relationship. Bioinformatics methods are also used to assign functions to uncharacterized genes which can be targeted as a candidate in vaccine design and can be a better approach toward the inclusion of women that are pregnant into vaccine trials and programs. The essence of this review is to give insight into the need to focus on novel computational, experimental and computation-driven experimental approaches for studying of host-pathogen interactions and thus making a case for its use in vaccine development.

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“… 10 Moreover, they have been unable to confer protection against numerous emerging and re-emerging infections. 21 Therefore, thinking of NTDs context of high medical urgency and low investment, new strategies involving shorter development times and lower costs would be required.…”

Section: Next Generation Vaccinology For Ntdsmentioning

confidence: 99%

An Overview of Current Uses and Future Opportunities for Computer-Assisted Design of Vaccines for Neglected Tropical Diseases

Robleda-Castillo¹,

Ros-Lucas²,

Martínez-Peinado³

et al. 2021

AABC

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Neglected tropical diseases are infectious diseases that impose high morbidity and mortality rates over 1.5 billion people worldwide. Originally restricted to tropical and subtropical regions, changing climate conditions have increased their potential to emerge elsewhere. Control of their impact suffers from shortages like poor epidemiological surveillance or irregular drug distribution, and some NTDs still lack of appropriate diagnostics and/or efficient therapeutics. For these, availability of vaccines to prevent new infections, or the worsening of those already established, would mean a major breakthrough. However, only dengue and rabies count with approved vaccines at present. Herein, we review the state-of-the-art of vaccination strategies for NTDs, setting the focus on third generation vaccines and the concept of reverse vaccinology. Its capability to address pathogens´ biological complexity, likely contributing to save developmental costs is discussed. The use of computational tools is a fundamental aid to analyze increasingly large datasets aimed at designing vaccine candidates with the highest, possibly, opportunities to succeed. Ultimately, we identify and analyze those studies that took an in silico approach to find vaccine candidates, and experimentally assessed their immunogenicity and/or protection capabilities.

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Exploitation of reverse vaccinology and immunoinformatics as promising platform for genome-wide screening of new effective vaccine candidates against Plasmodium falciparum

Cited by 31 publications

References 65 publications

Designing of multiepitope-based vaccine against Leptospirosis using Immuno-Informatics approaches

Designing of multiepitope-based vaccine against Leptospirosis using Immuno-Informatics approaches

<p>Immunoinformatics and Vaccine Development: An Overview</p>

An Overview of Current Uses and Future Opportunities for Computer-Assisted Design of Vaccines for Neglected Tropical Diseases

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