Explaining Unsaturated Fatty Acids (UFAs), Especially Polyunsaturated Fatty Acid (PUFA) Content in Subcutaneous Fat of Yaks of Different Sex by Differential Proteome Analysis

Xiong, Lin; Pei, Jie; Wu, Xiaoyun; Bao, Pengjia; Guo, Xian; Yan, Ping

doi:10.3390/genes13050790

Cited by 6 publications

(3 citation statements)

References 49 publications

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“…Currently, underlying component content and profiling, many useful methods have been developed to identify proteins and peptides at the transcriptomic, peptidomic, or genomic level to analyze the active substances, reveal the quality markers in medicinal materials, and perform an overall quality evaluation through bioinformatics and validation [33][34][35][36]. Here, transcriptomics and tandem mass tag (TMT) proteomics were used to analyze differences and identify quality markers in protein components of toad venom from different regions.…”

Section: Introductionmentioning

confidence: 99%

Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans

et al. 2023

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Toad venom is a traditional Chinese medicine with high medicinal value. The existing quality evaluation standards of toad venom have obvious limitations because of the lack of research on proteins. Thus, it is necessary to screen suitable quality markers and establish appropriate quality evaluation methods for toad venom proteins to guarantee their safety and efficacy in clinical applications. SDS-PAGE, HPLC, and cytotoxicity assays were used to analyze differences in protein components of toad venom from different areas. Functional proteins were screened as potential quality markers by proteomic and bioinformatic analyses. The protein components and small molecular components of toad venom were not correlated in content. Additionally, the protein component had strong cytotoxicity. Proteomics analysis showed that 13 antimicrobial proteins, four anti-inflammatory and analgesic proteins, and 20 antitumor proteins were differentially expressed extracellular proteins. A candidate list of functional proteins was coded as potential quality markers. Moreover, Lysozyme C-1, which has antimicrobial activity, and Neuropeptide B (NPB), which has anti-inflammatory and analgesic activity, were identified as potential quality markers for toad venom proteins. Quality markers can be used as the basis of quality studies of toad venom proteins and help to construct and improve safe, scientific, and comprehensive quality evaluation methods.

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Section: Introductionmentioning

confidence: 99%

Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans

et al. 2023

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“…Diacyl glyceryl transferase-2 (DGAT2) catalyzes the final reaction of TG synthesis using diacylglycerol and fatty acyl-CoA as substrates [ 30 ]. HACD3 catalyzes the dehydration of 3-hydroxy acyl-coA intermediates to trans—2, 3-dilute acyl-CoA, catalyzes the extended circulation of long-chain fatty acids, and promotes the transport and synthesis of unsaturated fatty acids (UFAs) [ 31 ]. Fatty acid desaturase 1 (FADS1), as a key enzyme in the metabolism of polyunsaturated fatty acids (PUFA), catalyzes di-high -y-linolenic acid (DGLA) into arachidonic acid (AA) [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Cold Exposure Regulates Hepatic Glycogen and Lipid Metabolism in Newborn Goats

Su,

Zhou,

Wang

et al. 2023

IJMS

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Cold exposure influences liver metabolism, thereby affecting energy homeostasis. However, the gene regulatory network of the liver after cold exposure remains poorly understood. In this study, we found that 24 h cold exposure (COLD, 6 °C) increased plasma glucose (GLU) levels, while reducing plasma non-esterified fatty acid (NEFA) and triglyceride (TG) levels compared to the room temperature (RT, 25 °C) group. Cold exposure increased hepatic glycogen content and decreased hepatic lipid content in the livers of newborn goats. We conducted RNA-seq analysis on the livers of newborn goats in both the RT and cold exposure groups. A total of 1600 genes were identified as differentially expressed genes (DEGs), of which 555 genes were up-regulated and 1045 genes were down-regulated in the cold exposure group compared with the RT group. Cold exposure increased the expression of genes involved in glycolysis, glycogen synthesis, and fatty acid degradation pathways. These results can provide a reference for hepatic lipid and glycogen metabolism in newborn goats after cold exposure.

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“…Therefore, the diet determines the concentrations of fatty acids in adipose tissue. Some tests suggest a homogenous composition of fat throughout the body [ 29 ], however, Xiong et al [ 30 ] detected a higher content of PUFA in subcutaneous fat, which might explain why the superficial or subcutaneous fat is softer than the deeper fat. The difference in composition between subcutaneous, abdominal fat, and omental fat might indicate that the relations of these different adipose regions with lipolytic activity also differ.…”

Section: Discussionmentioning

confidence: 99%

Green cabbage supplementation influences the gene expression and fatty acid levels of adipose tissue in Chinese Wanxi White geese

Wang¹,

Liu²,

Chen³

et al. 2023

Anim Biosci

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Objective: Dietary green cabbage was evaluated for its impact on fatty acid synthetic ability in different adipose tissues during fattening of Wanxi White geese.Methods: A total of 256 Wanxi White geese at their 70 days were randomly allocated into 4 groups with 4 replicates and fed 0%, 15%, 30%, and 45% fresh green cabbage (relative to dry matter), respectively, in each group. Adipose tissues (subcutaneous and abdominal fat), liver and blood were collected from 4 birds in each replicate at their 70, 80, 90, and 100 days for fatty acid composition, relative gene expression and serum lipid analysis. Two-way or three-way analysis of variance was used for analysis.Results: The contents of palmitic acid (C16:0), palmitoleic acid (C16:1), linoleic acid (C18:2), and alpha-linolenic acid (C18:3) were feeding time dependently increased. The C16:0 and stearic acid (C18:0) were higher in abdominal fat, while C16:1, oleic acid (C18:1), and C18:2 were higher in subcutaneous fat. Geese fed 45% green cabbage exhibited highest level of C18:3. Geese fed green cabbage for 30 d exhibited higher level of C16:0 and C18:0 in abdominal fat, while geese fed 30% to 45% green cabbage exhibited higher C18:3 in subcutaneous fat. The expression of Acsl1 (p = 0.003) and Scd1 (p<0.0001) were decreased with green cabbage addition. Interaction between feeding time and adipose tissue affected elongation of long-chain fatty acids family member 6 (Elovl6), acyl-CoA synthetase longchain family member 1 (Acsl1), and stearoly-coA desaturase 1 (Scd1) gene expression levels (p = 0.013, p = 0.003, p = 0.005). Feeding time only affected serum lipid levels of free fatty acid and chylomicron. Higher contents of C16:0, C18:1, and C18:3 were associated with greater mRNA expression of Scd1 (p<0.0001), while higher level of C18:2 was associated with less mRNA expression of Scd1 (p<0.0001).Conclusion: Considering content of C18:2 and C18:3, 30% addition of green cabbage could be considered for fattening for 30 days in Wanxi White geese.

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Explaining Unsaturated Fatty Acids (UFAs), Especially Polyunsaturated Fatty Acid (PUFA) Content in Subcutaneous Fat of Yaks of Different Sex by Differential Proteome Analysis

Cited by 6 publications

References 49 publications

Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans

Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans

Cold Exposure Regulates Hepatic Glycogen and Lipid Metabolism in Newborn Goats

Green cabbage supplementation influences the gene expression and fatty acid levels of adipose tissue in Chinese Wanxi White geese

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